@misc{HerzogHansenMiethbaueretal., author = {Herzog, Natalie and Hansen, Max and Miethbauer, Sebastian and Schmidtke, Kai-Uwe and Anderer, Ursula and Lupp, Amelie and Sperling, Sebastian and Seehofer, Daniel and Damm, Georg and Scheibner, Katrin and K{\"u}pper, Jan-Heiner}, title = {Primary-like human hepatocytes genetically engineered to obtain proliferation competence display hepatic differentiation characteristics in monolayer and organotypical spheroid cultures}, series = {Cell Biology International}, volume = {40}, journal = {Cell Biology International}, number = {3}, issn = {1095-8355}, doi = {10.1002/cbin.10574}, pages = {341 -- 353}, abstract = {Primary human hepatocytes are in great demand during drug development and in hepatology. However, both scarcity of tissue supply and donor variability of primary cells create a need for the development of alternative hepatocyte systems. By using a lentivirus vector system to transfer coding sequences of Upcyte® proliferation genes, we generated non-transformed stable hepatocyte cultures from human liver tissue samples. Here, we show data on newly generated proliferation-competent HepaFH3 cells investigated as conventional two-dimensional monolayer and as organotypical three-dimensional (3D) spheroid culture. In monolayer culture, HepaFH3 cells show typical cobblestone-like hepatocyte morphology and anchorage-dependent growth for at least 20 passages. Immunofluorescence staining revealed that characteristic hepatocyte marker proteins cytokeratin 8, human serum albumin, and cytochrome P450 (CYP) 3A4 were expressed. Quantitative real-time PCR analyses showed that expression levels of analyzed phase I CYP enzymes were at similar levels compared to those of cultured primary human hepatocytes and considerably higher than in the liver carcinoma cell line HepG2. Additionally, transcripts for phase II liver enzymes and transporter proteins OATP-C, MRP2, Oct1, and BSEP were present in HepaFH3. The cells produced urea and converted model compounds such as testosterone, diclofenac, and 7-OH-coumarin into phases I and II metabolites. Interestingly, phases I and II enzymes were expressed at about the same levels in convenient monolayer cultures and complex 3D spheroids. In conclusion, HepaFH3 cells and related primary-like hepatocyte lines seem to be promising tools for in vitro research of liver functions and as test system in drug development and toxicology analysis.}, language = {en} } @misc{KadeHerzogSchmidtkeetal., author = {Kade, S. and Herzog, Natalie and Schmidtke, Kai-Uwe and K{\"u}pper, Jan-Heiner}, title = {Chronic ethanol treatment depletes glutathione regeneration capacity in hepatoma cell line HepG2}, series = {Journal of Cellular Biotechnology}, volume = {1}, journal = {Journal of Cellular Biotechnology}, number = {2}, issn = {2352-3697}, doi = {10.3233/JCB-15019}, pages = {183 -- 190}, language = {en} } @misc{HerzogHansenMiethbaueretal., author = {Herzog, Natalie and Hansen, Max and Miethbauer, Sebastian and Schmidtke, Kai-Uwe and Anderer, Ursula and Lupp, Amelie and Sperling, Sebastian and Seehofer, Daniel and Damm, Georg and Scheibner, Katrin and K{\"u}pper, Jan-Heiner}, title = {Primary like human hepatocytes genetically engineered to obtain proliferation competence display liver biotransformation activity in 2D and 3D culture systems}, series = {Cell Biology International}, volume = {40}, journal = {Cell Biology International}, number = {3}, issn = {1095-8355}, pages = {341 -- 353}, language = {en} } @misc{GeorgeRoedigerSchroederetal., author = {George, Sandra and R{\"o}diger, Stefan and Schr{\"o}der, Christian and Knaut, Michael and K{\"u}pper, Jan-Heiner}, title = {Development of multiplex PCR systems for expression profiling of human cardiomyocytes induced to profilerate by lentivirus transduction of upcyte genes}, series = {Journal of Cellular Biotechnology}, volume = {2}, journal = {Journal of Cellular Biotechnology}, number = {1}, issn = {2352-3697}, doi = {10.3233/JCB-15025}, pages = {35 -- 55}, language = {en} } @misc{MeyerMeyerFiccaKuepper, author = {Meyer, Ralph G. and Meyer-Ficca, Mirella and K{\"u}pper, Jan-Heiner}, title = {Adenoviral vectors for modulation of poly(ADP-ribose) polymerase-1 (PARP1) - dependent DNA repair as a predictive tool for chemotherapy}, series = {Journal of Cellular Biotechnology}, volume = {2}, journal = {Journal of Cellular Biotechnology}, number = {1}, issn = {2352-3697}, doi = {10.3233/JCB-15026}, pages = {57 -- 68}, language = {en} } @misc{HerzogKatzenbergerMartinetal., author = {Herzog, Natalie and Katzenberger, Nadine and Martin, Frank and Schmidtke, Kai-Uwe and K{\"u}pper, Jan-Heiner}, title = {Generation of cytochrome P450 3A4-overexpressing HepG2 cell clones for standardization of hepatocellular testosterone 6β-hydroxylation activity}, series = {Journal of Cellular Biotechnology}, volume = {1}, journal = {Journal of Cellular Biotechnology}, number = {1}, issn = {2352-3697}, doi = {10.3233/JCB-15002}, pages = {15 -- 26}, language = {en} } @misc{GeorgeNoackVaneketal., author = {George, Sandra and Noack, M. and Vanek, Monika and Rentzsch, J. and R{\"o}ber, Nadja and Roggenbuck, Dirk and Conrad, Karsten and K{\"u}pper, Jan-Heiner}, title = {Expression of nicotinic acetylcholine receptor subunits in HEp-2 cells for immunodetection of autoantibody specificities in sera fro Myasthenia gravis patients}, series = {Clinical Hemorheology and Microcirculation}, volume = {61}, journal = {Clinical Hemorheology and Microcirculation}, number = {2}, issn = {1875-8622}, doi = {10.3233/CH-151999}, pages = {385 -- 396}, language = {en} } @misc{GeorgePaulickKnuetteretal., author = {George, Sandra and Paulick, Silvia and Kn{\"u}tter, Ilka and R{\"o}ber, Nadja and Hiemann, Rico and Roggenbuck, Dirk and Conrad, Karsten and K{\"u}pper, Jan-Heiner}, title = {Stable Expression of Human Muscle-Specific Kinase in HEp-2 M4 Cells for Automatic Immunofluorescence Diagnostics of Myasthenia Gravis}, series = {PLOS one}, volume = {9}, journal = {PLOS one}, number = {1}, issn = {1932-6203}, doi = {10.1371/journal.pone.0083924}, pages = {e83924}, language = {en} } @misc{GeorgeGeorgiRoggenbucketal., author = {George, Sandra and Georgi, M. and Roggenbuck, Dirk and Conrad, Karsten and K{\"u}pper, Jan-Heiner}, title = {A strategy for cell-based multiplex diagnostics of Myasthenia gravis and autoimmune encephalitis by modifying the subcellular localization of cell membrane autoantigensgravis}, series = {Clinical Hemorheology and Microcirculation}, volume = {58}, journal = {Clinical Hemorheology and Microcirculation}, number = {1}, issn = {1386-0291}, doi = {10.3233/CH-141897}, pages = {211 -- 228}, language = {en} } @incollection{ConradKuepper, author = {Conrad, Karsten and K{\"u}pper, Jan-Heiner}, title = {Tumor-associated autoantibodies}, series = {Autoantibodies}, booktitle = {Autoantibodies}, editor = {Shoenfeld, Yehuda and Meroni, Pier Luigi and Gershwin, M. Eric}, edition = {3rd edition}, publisher = {Elsevier}, address = {Amsterdam [u.a.]}, isbn = {978-0-444-56378-1}, pages = {275 -- 287}, language = {en} }