@misc{VagiannisBudagagaMorelletal., author = {Vagiannis, Dimitrios and Budagaga, Youssif and Morell, Anselm and Zhang, Yu and Novotna, Eva and Skarka, Adam and Kammerer, Sarah and K{\"u}pper, Jan-Heiner and Hanke, Ivo and Rozkoš, Tom{\´a}š and Hofman, Jakub}, title = {Tepotinib Inhibits Several Drug Efflux Transporters and Biotransformation Enzymes: The Role in Drug-Drug Interactions and Targeting Cytostatic Resistance In Vitro and Ex Vivo}, series = {International Journal of Molecular Sciences}, volume = {22}, journal = {International Journal of Molecular Sciences}, number = {21}, issn = {1422-0067}, doi = {10.3390/ijms222111936}, language = {en} } @misc{JungWaldeckSykoraetal., author = {Jung, Conrad H. G. and Waldeck, Peter and Sykora, Shadi and Braune, Steffen and Petrick, Ingolf and K{\"u}pper, Jan-Heiner and Jung, Friedrich}, title = {Influence of Different Light-Emitting Diode Colors on Growth and Phycobiliprotein Generation of Arthrospira platensis}, series = {Life}, volume = {12}, journal = {Life}, number = {6}, issn = {2075-1729}, doi = {10.3390/life12060895}, language = {en} } @misc{LemmKoehlerWodtkeetal., author = {Lemm, Sandy and K{\"o}hler, Susanne and Wodtke, Robert and Jung, Friedrich and K{\"u}pper, Jan-Heiner and Pietzsch, Jens and Laube, Markus}, title = {Investigation of radiotracer metabolic stability in vitro with CYP-overexpressing hepatoma cell lines}, series = {Cells}, volume = {11}, journal = {Cells}, number = {15}, issn = {2073-4409}, doi = {10.3390/cells11152447}, language = {en} } @misc{JungBrauneJungetal., author = {Jung, Friedrich and Braune, Steffen and Jung, Conrad H. G. and Kr{\"u}ger-Genge, Anne and Waldeck, Peter and Petrick, Ingolf and K{\"u}pper, Jan-Heiner}, title = {Lipophilic and Hydrophilic Compounds from Arthrospira platensis and Its Effects on Tissue and Blood Cells—An Overview}, series = {Life}, volume = {12}, journal = {Life}, number = {10}, issn = {2075-1729}, doi = {10.3390/life12101497}, language = {en} } @misc{BrauneKruegerGengeKoehleretal., author = {Braune, Steffen and Kr{\"u}ger-Genge, Anne and K{\"o}hler, S. and K{\"u}pper, Jan-Heiner and Jung, Friedrich}, title = {Effects of Arthrospira platensis-derived substances on blood cells}, series = {Clinical Hemorheology and Microcirculation}, volume = {85(2023)}, journal = {Clinical Hemorheology and Microcirculation}, number = {3}, issn = {1875-8622}, doi = {10.3233/CH-229103}, pages = {315 -- 321}, language = {en} } @misc{AbdelfatahNassKnorzetal., author = {Abdelfatah, Sara and Nass, Janine and Knorz, Caroline and Klauck, Sabine M. and K{\"u}pper, Jan-Heiner and Efferth, Thomas}, title = {Pyrrolizidine alkaloids cause cell cycle and DNA damage repair defects as analyzed by transcriptomics in cytochrome P450 3A4-overexpressing HepG2 clone 9 cells}, series = {Cell biology and toxicology}, volume = {38}, journal = {Cell biology and toxicology}, number = {2}, issn = {1573-6822}, doi = {10.1007/s10565-021-09599-9}, pages = {325 -- 345}, language = {en} } @misc{HaasWirachowskiKuepperetal., author = {Haas, M. and Wirachowski, K. and K{\"u}pper, Jan-Heiner and Schrenk, Dieter and Fahrer, J.}, title = {Structure-dependent genotoxicity and cytotoxicity of eleven pyrrolizidine alkaloids in human liver cells with CYP3A4 overexpression}, series = {Lebensmittelchemie : Volume 75: Vortr{\"a}ge und Poster des 49. Deutschen Lebensmittelchemikertags}, journal = {Lebensmittelchemie : Volume 75: Vortr{\"a}ge und Poster des 49. Deutschen Lebensmittelchemikertags}, issn = {0937-1478}, doi = {10.1002/lemi.202158135}, pages = {S134}, language = {en} } @misc{LemmKoehlerWodtkeetal., author = {Lemm, Sandy and K{\"o}hler, Susanne and Wodtke, Robert and Jung, Friedrich and K{\"u}pper, Jan-Heiner and Pietzsch, Jens and Laube, Markus}, title = {Enabling insights into the cytochrome P450 isoform specific metabolism of radiotracers by CYP overexpressing hepatoblastoma cell lines}, series = {Nuclear Medicine and Biology}, journal = {Nuclear Medicine and Biology}, number = {108-109, Supplement }, issn = {1872-9614}, pages = {S66 -- S67}, language = {en} } @misc{BuchmuellerEngePetersetal., author = {Buchmueller, Julia and Enge, Anne-Margarethe and Peters, Antonia and Ebmeyer, Johanna and K{\"u}pper, Jan-Heiner and Sch{\"a}fer, Bernd and Br{\"a}uning, Albert and Hessel-Pras, Stefanie}, title = {The chemical structure impairs the intensity of genotoxic effects promoted by 1,2-unsaturated pyrrolizidine alkaloids in vitro}, series = {Food and Chemical Toxicology}, volume = {Vol. 164}, journal = {Food and Chemical Toxicology}, issn = {1873-6351}, doi = {10.1016/j.fct.2022.113049}, language = {en} } @misc{KnauerSchulzZemellaetal., author = {Knauer, Jan Felix and Schulz, Christian and Zemella, Anne and W{\"u}stenhagen, Doreen A. and Walter, Ruben Magnus and K{\"u}pper, Jan-Heiner and Kubick, Stefan}, title = {Synthesis of mono Cytochrome P450 in a modified CHO-CPR cell-free protein production platform}, series = {Scientific Reports}, volume = {14}, journal = {Scientific Reports}, issn = {2045-2322}, doi = {10.1038/s41598-024-51781-6}, pages = {14}, abstract = {AbstractCytochromes P450 (CYPs) are a group of monooxygenases that can be found in almost all kinds of organisms. For CYPs to receive electrons from co-substrate NADPH, the activity of NADPH-Cytochrome-P450-oxidoreductase (CPR) is required as well. In humans, CYPs are an integral part of liver-based phase-1 biotransformation, which is essential for the metabolization of multiple xenobiotics and drugs. Consequently, CYPs are important players during drug development and therefore these enzymes are implemented in diverse screening applications. For these applications it is usually advantageous to use mono CYP microsomes containing only the CYP of interest. The generation of mono-CYP containing mammalian cells and vesicles is difficult since endogenous CYPs are present in many cell types that contain the necessary co-factors. By obtaining translationally active lysates from a modified CHO-CPR cell line, it is now possible to generate mono CYPs in a cell-free protein synthesis process in a straightforward manner. As a proof of principle, the synthesis of active human CYPs from three different CYP450 gene families (CYP1A2, CYP2B6 and CYP3A4), which are of outstanding interest in industry and academia was demonstrated. Luciferase based activity assays confirm the activity of the produced CYPs and enable the individual adaptation of the synthesis process for efficient cell-free enzyme production. Furthermore, they allow for substrate and inhibitor screenings not only for wild-type CYPs but also for mutants and further CYP isoforms and variants. As an example, the turnover of selected CYP substrates by cell-free synthesized CYPs was demonstrated via an indirect luciferase assay-based screening setup.}, language = {en} }