@misc{HofmanSorfVagiannisetal., author = {Hofman, Jakub and Sorf, Ales and Vagiannis, Dimitrios and Sucha, Simona and Novotna, Eva and Kammerer, Sarah and K{\"u}pper, Jan-Heiner and Ceckova, Martina and Staud, Frantisek}, title = {Interactions of Alectinib with Human ATP-Binding Cassette Drug Efflux Transporters and Cytochrome P450 Biotransformation Enzymes: Effect on Pharmacokinetic Multidrug Resistance}, series = {Drug Metabolism and Disposition}, volume = {47}, journal = {Drug Metabolism and Disposition}, number = {7}, issn = {1521-009X}, doi = {10.1124/dmd.119.086975}, pages = {699 -- 709}, language = {en} } @misc{SteinbrechtKammererKuepper, author = {Steinbrecht, Susanne and Kammerer, Sarah and K{\"u}pper, Jan-Heiner}, title = {HepG2 cells with recombinant cytochrome P450 enzyme overexpression: Their use and limitation as in vitro liver model}, series = {Journal of Cellular Biotechnology}, volume = {5}, journal = {Journal of Cellular Biotechnology}, number = {1}, issn = {2352-3697}, doi = {10.3233/JCB-189013}, pages = {55 -- 64}, language = {en} } @misc{HofmanSorfVagiannisetal., author = {Hofman, Jakub and Sorf, Ales and Vagiannis, Dimitrios and Sucha, Simona and Kammerer, Sarah and K{\"u}pper, Jan-Heiner and Chen, Si and Guo, Lei and Ceckova, Martina and Staud, Frantisek}, title = {Brivanib Exhibits Potential for Pharmacokinetic Drug-Drug Interactions and the Modulation of Multidrug Resistance through the Inhibition of Human ABCG2 Drug Efflux Transporter and CYP450 Biotransformation Enzymes}, series = {Molecular Pharmaceutics}, volume = {16}, journal = {Molecular Pharmaceutics}, number = {11}, doi = {10.1021/acs.molpharmaceut.9b00361}, pages = {4436 -- 4450}, language = {en} } @misc{HailekaGeorgeSteinbrechtetal., author = {Haileka, Vanessa and George, Sandra and Steinbrecht, Susanne and Jung, Friedrich and Reinehr, R. and K{\"u}pper, Jan-Heiner}, title = {Colon cancer cells cultured under hyperosmotic conditions as in vitro model to investigate dehydration effects on cancer drug susceptibility}, series = {Clinical Hemorheology and Microcirculation}, volume = {73}, journal = {Clinical Hemorheology and Microcirculation}, number = {1}, issn = {1875-8622}, doi = {10.3233/CH-199210}, pages = {169 -- 176}, language = {en} } @misc{SchulzKammererKuepper, author = {Schulz, Christian and Kammerer, Sarah and K{\"u}pper, Jan-Heiner}, title = {NADPH-cytochrome P450 reductase expression and enzymatic activity in primary-like human hepatocytes and HepG2 cells for in vitro biotransformation studies}, series = {Clinical Hemorheology and Microcirculation}, volume = {73}, journal = {Clinical Hemorheology and Microcirculation}, number = {1}, issn = {1386-0291}, doi = {10.3233/CH-199226}, pages = {249 -- 260}, language = {en} } @misc{UhligGehreKammereretal., author = {Uhlig, Katja and Gehre, Christian P. and Kammerer, Sarah and K{\"u}pper, Jan-Heiner and Coleman, D. and P{\"u}schel, G. and Duschl, Claus}, title = {Real-time monitoring of oxygen consumption of hepatocytes in a microbioreactor}, series = {Toxicology Letters}, volume = {295}, journal = {Toxicology Letters}, number = {Supplement 1}, issn = {0378-4274}, doi = {10.1016/j.toxlet.2018.06.652}, pages = {S115}, language = {en} } @misc{SteinbrechtPfeiferHerzogetal., author = {Steinbrecht, Susanne and Pfeifer, Nadine and Herzog, Natalie and Katzenberger, Nadine and Schulz, Christian and Kammerer, Sarah and K{\"u}pper, Jan-Heiner}, title = {HepG2-1A2 C2 and C7: Lentivirus vector-mediated stable and functional overexpression of cytochrome P450 1A2 in human hepatoblastoma cells}, series = {Toxicology Letters}, volume = {319}, journal = {Toxicology Letters}, issn = {0378-4274}, doi = {10.1016/j.toxlet.2019.11.006}, pages = {155 -- 159}, abstract = {Novel HepG2 cell clones 1A2 C2 and 1A2 C7 were independently generated by lentiviral transduction to functionally overexpress cytochrome P450 1A2 (CYP1A2). We found similar and stable CYP1A2 transcript and protein levels in both cell clones leading to specific enzyme activities of about 370 pmol paracetamol x min-1 x mg-1 protein analyzed by phenacetin conversion. Both clones showed dramatically increased sensitivity to the hepatotoxic compound aflatoxin B1 (EC50<100 nM) when compared to parental HepG2 cells (EC50 ∼5 μM). Thus, newly established cell lines are an appropriate tool to study metabolism and toxicity of substances depending on conversion by CYP1A2.}, language = {en} } @misc{LauRangarajanKruegerGengeetal., author = {Lau, S. and Rangarajan, R. and Kr{\"u}ger-Genge, Anne and Braune, Steffen and Kammerer, Sarah and K{\"u}pper, Jan-Heiner and Lendlein, Andreas and Jung, Friedrich}, title = {Effects of acrolein in comparison to its prodrug cyclophosphamide on human primary endothelial cells in vitro}, series = {Toxicology in Vitro}, volume = {62}, journal = {Toxicology in Vitro}, issn = {0887-2333}, doi = {10.1016/j.tiv.2019.104685}, pages = {8}, abstract = {Cyclophosphamide (CPA) is one of the most successful anticancer prodrugs that becomes effective after biotransformation in the liver resulting in the toxic metabolite acrolein. Cancer is often accompanied by thromboembolic events, which might be a result of dysfunctional endothelial cells due to CPA treatment. Here, the effect of 1 mM CPA or acrolein (10/50/100/500 μM) on human umbilical vein endothelial cells (HUVECs) was analyzed after two days of treatment. The addition of CPA or 10 μM acrolein did not affect HUVECs. However, concentrations of 100 μM and 500 μM acrolein significantly reduced the number of adherent cells by 86 ± 13\% and 99 ± 1\% and cell viability by 51 ± 29\% and 93 ± 8\% compared to the control. Moreover, pronounced stress fibers as well as multiple nuclei were observed and von Willebrand factor (vWF) was completely released. Lactate dehydrogenase was 8.5 ± 7.0-fold and 252.9 ± 42.9-fold increased showing a loss of cell membrane integrity. The prostacyclin and thromboxane secretion was significantly increased by the addition of 500 μM acrolein (43.1 ± 17.6-fold and 246.4 ± 106.3-fold) indicating cell activation/pertubation. High doses of acrolein led to HUVEC death and loss of vWF production. This effect might be associated with the increased incidence of thromboembolic events in cancer patients treated with high doses of CPA.}, language = {en} } @misc{JungKruegerGengeWaldecketal., author = {Jung, Friedrich and Kr{\"u}ger-Genge, Anne and Waldeck, Peter and K{\"u}pper, Jan-Heiner}, title = {Spirulina platensis, a super food?}, series = {Journal of Cellular Biotechnology}, volume = {5}, journal = {Journal of Cellular Biotechnology}, number = {1}, issn = {2352-3689}, doi = {10.3233/JCB-189012}, pages = {43 -- 54}, abstract = {Spirulina platensis, a multicelluar, photosynthetic prokaryote (algae) contains a high amount of proteins, vitamins and minerals superior to many foods as e.g. soybeans. Thus, Spirulina platensis was recognized as nutritious food by the United Nations World Food Conference. Due to the high amount of nutritive ingredients Spirulina has a long history as dietary supplement. In addition, spirulina platensis is also efficiently used as forage with known effects on flesh, egg and plumage color, milk yield and fertility. The versatile utilization of the alga can be explained on the one hand with the nutrient levels and on the other hand with recognized effects as anti-viral, anti-bacterial, anti-oxidant, anti-diabetic, anti-cancer and anti-inflammatory substance. Therefore, this alga is named as "superfood". Beyond, these algae convert carbon dioxide into organic substances and produce oxygen during their growth in alkaline and saline water thereby not wasting fresh water allowing the production in barren areas. Despite this diverse use of Spirulina platensis due to its beneficial properties, many basic mechanisms on a molecular and cellular level are not well understood and should be explored in future studies.}, language = {en} } @misc{KruegerGengeJungKuepperetal., author = {Kr{\"u}ger-Genge, Anne and Jung, Friedrich and K{\"u}pper, Jan-Heiner and Lehmann, Christian and Franke, Ralf-Peter}, title = {Actin type and distribution in erythrocytes}, series = {Journal of Cellular Biotechnology}, volume = {3}, journal = {Journal of Cellular Biotechnology}, number = {2}, issn = {2352-3697}, doi = {10.3233/JCB-179014}, pages = {81 -- 83}, abstract = {Erythrocytes transport oxygen from the lungs to the tissues. The excess surface area together with the elasticity of the erythrocyte cell membrane provides the flexibility needed to pass through the microvasculature where the oxygen exchange occurs. Although the architecture of the red cell and its membrane-associated cytoskeletal network is known in general, the factors that control the characteristic shape change during echinocyte formation are poorly understood. In this short report we show that in echinocytes a completely reorganized membrane cytoskeleton with a box-like structure of actin filaments prevailed indicating the importance of the actin cytoskeleton during echinocyte formation.}, language = {en} }