@misc{CiglerKralKožišeketal., author = {C{\´i}gler, Petr and Kr{\´a}l, Vladim{\´i}r and Kož{\´i}šek, Milan and Konvalinka, Jan and Mirsky, Vladimir M.}, title = {Anomalous adsorptive properties of HIV protease: Indication of two-dimensional crystallization?}, series = {Colloids and Surfaces B: Biointerfaces}, volume = {64}, journal = {Colloids and Surfaces B: Biointerfaces}, number = {1}, issn = {0927-7765}, doi = {10.1016/j.colsurfb.2008.01.011}, pages = {145 -- 149}, abstract = {Adsorption of HIV protease onto surfaces that are usually considered to be protein-resistant was studied quantitatively using surface plasmon resonance. Adsorption onto gold surfaces functionalized by OH-terminated alkyl chains was much stronger than onto oligo(ethylene glycol)-terminated surfaces. Equilibrium and kinetic adsorption constants were determined. An anomalous mutual attraction between adsorbate molecules was observed, indicating the possibility of two-dimensional crystallization of HIV protease. These results are applicable for the design of sensors/biosensors for HIV protease resistance detection and for proper manipulation of this enzyme in laboratory devices.}, language = {en} }