@misc{GeorgePaulickKnuetteretal., author = {George, Sandra and Paulick, Silvia and Kn{\"u}tter, Ilka and R{\"o}ber, Nadja and Hiemann, Rico and Roggenbuck, Dirk and Conrad, Karsten and K{\"u}pper, Jan-Heiner}, title = {Stable Expression of Human Muscle-Specific Kinase in HEp-2 M4 Cells for Automatic Immunofluorescence Diagnostics of Myasthenia Gravis}, series = {PLOS one}, volume = {9}, journal = {PLOS one}, number = {1}, issn = {1932-6203}, doi = {10.1371/journal.pone.0083924}, pages = {e83924}, language = {en} } @misc{SowaGrossmannKnuetteretal., author = {Sowa, Mandy and Großmann, Kai and Kn{\"u}tter, Ilka and Hiemann, Rico and R{\"o}ber, Nadja and Anderer, Ursula and Csernok, Elena and Bogdanos, Dimitrios Petrou and Borghi, Maria Orietta and Meroni, Pier Luigi and Schierack, Peter and Reinhold, Dirk and Conrad, Karsten and Roggenbuck, Dirk}, title = {Simultaneous automated screening and confirmatory testing for vasculitis-specific ANCA}, series = {PLoS ONE}, volume = {9}, journal = {PLoS ONE}, number = {9}, issn = {1932-6203}, doi = {10.1371/journal.pone.0107743}, pages = {11}, language = {en} } @misc{KnuetterHiemannBrummaetal., author = {Kn{\"u}tter, Ilka and Hiemann, Rico and Brumma, Therese and B{\"u}ttner, Thomas and Großmann, Kai and Cusini, Marco and Pregnolato, Francesca and Borghi, Maria Orietta and Anderer, Ursula and Conrad, Karsten and Reinhold, Dirk and Roggenbuck, Dirk and Csernok, Elena}, title = {Automated interpretation of ANCA patterns - a new approach in the serology of ANCA-associated vasculitis}, series = {Arthritis Research \& Therapy}, volume = {14}, journal = {Arthritis Research \& Therapy}, issn = {1465-9913}, doi = {10.1186/ar4119}, pages = {10}, language = {en} } @misc{WillitzkiHiemannPetersetal., author = {Willitzki, Annika and Hiemann, Rico and Peters, Vanessa and Sack, Ulrich and Schierack, Peter and R{\"o}diger, Stefan and Anderer, Ursula and Conrad, Karsten and Bogdanos, Dimitrios Petrou and Reinhold, Dirk and Roggenbuck, Dirk}, title = {New PlatformTechnology for Comprehensive Serological Diagnostics of Autoimmune Diseases}, series = {Clinical and Developmental Immunology}, journal = {Clinical and Developmental Immunology}, issn = {1740-2530}, doi = {10.1155/2012/284740}, pages = {1 -- 8}, language = {en} } @misc{SchierackRoedigerKuhletal., author = {Schierack, Peter and R{\"o}diger, Stefan and Kuhl, Christoph and Hiemann, Rico and Roggenbuck, Dirk and Li, Ganwu and Weinreich, J{\"o}rg and Berger, Enrico and Nolan, Lisa K. and Nicholson, Bryon and R{\"o}mer, Antje and Fr{\"o}mmel, Ulrike and Wieler, Lothar H. and Schr{\"o}der, Christian}, title = {Porcine E. coli: Virulence-Associated Genes, Resistance Genes and Adhesion and Probiotic Activity Tested by a New Screening Method}, series = {PLoS One}, volume = {8}, journal = {PLoS One}, number = {4}, doi = {10.1371/journal.pone.0059242}, pages = {13}, abstract = {We established an automated screening method to characterize adhesion of Escherichia coli to intestinal porcine epithelial cells (IPEC-J2) and their probiotic activity against infection by enteropathogenic E. coli (EPEC). 104 intestinal E. coli isolates from domestic pigs were tested by PCR for the occurrence of virulence-associated genes, genes coding for resistances to antimicrobial agents and metals, and for phylogenetic origin by PCR. Adhesion rates and probiotic activity were examined for correlation with the presence of these genes. Finally, data were compared with those from 93 E. coli isolates from wild boars. Isolates from domestic pigs carried a broad variety of all tested genes and showed great diversity in gene patterns. Adhesions varied with a maximum of 18.3 or 24.2 mean bacteria adherence per epithelial cell after 2 or 6 hours respectively. Most isolates from domestic pigs and wild boars showed low adherence, with no correlation between adhesion/probiotic activity and E. coli genes or gene clusters. The gene sfa/foc, encoding for a subunit of F1C fimbriae did show a positive correlative association with adherence and probiotic activity; however E. coli isolates from wild boars with the sfa/foc gene showed less adhesion and probiotic activity than E. coli with the sfa/foc gene isolated from domestic pigs after 6 hour incubation. In conclusion, screening porcine E. coli for virulence associated genes genes, adhesion to intestinal epithelial cells, and probiotic activity revealed a single important adhesion factor, several probiotic candidates, and showed important differences between E. coli of domestic pigs and wild boars.}, language = {en} } @misc{RoggenbuckHiemannSchieracketal., author = {Roggenbuck, Dirk and Hiemann, Rico and Schierack, Peter and Reinhold, Dirk and Conrad, Karsten}, title = {Digital immunofluorescence enables automated detection of antinuclear antibody endpoint titers avoiding serial dilution}, series = {Clinical Chemistry and Laboratory Medicine}, volume = {52}, journal = {Clinical Chemistry and Laboratory Medicine}, number = {2}, issn = {1437-4331}, doi = {10.1515/cclm-2013-0543}, pages = {e9 -- e11}, language = {en} } @misc{WillitzkiLorenzHiemannetal., author = {Willitzki, Annika and Lorenz, Sebastian and Hiemann, Rico and Guttek, Karina and Goihl, Alexander and Hartig, Roland and Conrad, Karsten and Feist, Eugen and Sack, Ulrich and Schierack, Peter and Heiserich, Lisa and Eberle, Caroline and Peters, Vanessa and Roggenbuck, Dirk and Reinhold, Dirk}, title = {Fully automated analysis of chemically induced γH2AX foci in human peripheral blood mononuclear cells by indirect immunofluorescence}, series = {Cytometry Part A}, volume = {83}, journal = {Cytometry Part A}, number = {11}, issn = {1552-4930}, doi = {10.1002/cyto.a.22350}, pages = {1017 -- 1026}, language = {en} } @misc{SchierackKletaTedinetal., author = {Schierack, Peter and Kleta, Sylvia and Tedin, Karsten and Babila, T. B. and Oswald, S. and Oelschlaeger, T. A. and Hiemann, Rico and Paetzold, S. and Wieler, Lothar H.}, title = {E. coli Nissle 1917 affects Salmonella adhesion to porcine intestinal epithelial cells}, series = {PLoS One}, volume = {6}, journal = {PLoS One}, number = {2}, doi = {10.1371/journal.pone.0014712}, pages = {10}, language = {en} } @misc{SowaGrossmannScholzetal., author = {Sowa, Mandy and Großmann, Kai and Scholz, Juliane and R{\"o}ber, Nadja and R{\"o}diger, Stefan and Schierack, Peter and Conrad, Karsten and Roggenbuck, Dirk and Hiemann, Rico}, title = {Der CytoBead-Assay - Eine neue M{\"o}glichkeit der multiparametrischen Autoantik{\"o}rperanalytik bei systemischen Autoimmunerkrankungen}, series = {Journal of Laboratory Medicine}, volume = {38}, journal = {Journal of Laboratory Medicine}, number = {6}, doi = {10.1515/labmed-2014-0041}, pages = {309 -- 317}, language = {de} } @misc{SchierackRoedigerKolendaetal., author = {Schierack, Peter and R{\"o}diger, Stefan and Kolenda, Rafał and Hiemann, Rico and Berger, Enrico and Grzymajlo, Krzysztof and Swidsinski, Alexander and Juretzek, Thomas and Meissner, Dirk and Mydlak, Karsten and Reinhold, Dirk and Nolan, Lisa K. and Roggenbuck, Dirk}, title = {Species-specific and pathotype-specific binding of bacteria to zymogen granule membrane glycoprotein 2 (GP2)}, series = {Gut}, volume = {64}, journal = {Gut}, number = {3}, issn = {1468-3288}, doi = {10.1136/gutjnl-2014-307854}, pages = {517 -- 519}, language = {en} }