@incollection{HofrichterKellnerHerzogetal., author = {Hofrichter, Martin and Kellner, Harald and Herzog, Robert and Karich, Alexander and Liers, Christiane and Scheibner, Katrin and Kimani, Virginia Wambui and Ullrich, Ren{\´e}}, title = {Fungal Peroxygenases: A Phylogenetically Old Superfamily of Heme Enzymes with Promiscuity for Oxygen Transfer Reactions.}, series = {Grand Challenges in Fungal Biotechnology}, booktitle = {Grand Challenges in Fungal Biotechnology}, editor = {Nevalainen, Helena}, edition = {1. Auflage}, publisher = {Springer Nature}, address = {Cham}, isbn = {978-3-030-29540-0}, issn = {2367-1017}, doi = {10.1007/978-3-030-29541-7}, pages = {369 -- 403}, language = {en} } @misc{HofrichterKellnerHerzogetal., author = {Hofrichter, Martin and Kellner, Harald and Herzog, Robert and Karich, Alexander and Kiebist, Jan and Scheibner, Katrin and Ullrich, Ren{\´e}}, title = {Peroxide-Mediated Oxygenation of Organic Compounds by Fungal Peroxygenases}, series = {Antioxidants}, volume = {11}, journal = {Antioxidants}, number = {1}, issn = {2076-3921}, doi = {10.3390/antiox11010163}, pages = {1 -- 21}, abstract = {Unspecific peroxygenases (UPOs), whose sequences can be found in the genomes of thousands of filamentous fungi, many yeasts and certain fungus-like protists, are fascinating biocatalysts that transfer peroxide-borne oxygen (from H2O2 or R-OOH) with high efficiency to a wide range of organic substrates, including less or unactivated carbons and heteroatoms. A twice-proline-flanked cysteine (PCP motif) typically ligates the heme that forms the heart of the active site of UPOs and enables various types of relevant oxygenation reactions (hydroxylation, epoxidation, subsequent dealkylations, deacylation, or aromatization) together with less specific one-electron oxidations (e.g., phenoxy radical formation). In consequence, the substrate portfolio of a UPO enzyme always combines prototypical monooxygenase and peroxidase activities. Here, we briefly review nearly 20 years of peroxygenase research, considering basic mechanistic, molecular, phylogenetic, and biotechnological aspects.}, language = {en} } @misc{KoenigKiebistKalmbachetal., author = {K{\"o}nig, Rosalie and Kiebist, Jan and Kalmbach, Johannes and Herzog, Robert and Schmidtke, Kai-Uwe and Kellner, Harald and Ullrich, Ren{\´e} and Jehmlich, Nico and Hofrichter, Martin and Scheibner, Katrin}, title = {Novel unspecific peroxygenase from Truncatella angustata catalyzes the synthesis of bioactive lipid mediators}, series = {Microorganisms}, volume = {10}, journal = {Microorganisms}, number = {7}, issn = {2076-2607}, doi = {10.3390/microorganisms10071267}, pages = {1 -- 18}, abstract = {Lipid mediators, such as epoxidized or hydroxylated eicosanoids (EETs, HETEs) of arachidonic acid (AA), are important signaling molecules and play diverse roles at different physiological and pathophysiological levels. The EETs and HETEs formed by the cytochrome P450 enzymes are still not fully explored, but show interesting anti-inflammatory properties, which make them attractive as potential therapeutic target or even as therapeutic agents. Conventional methods of chemical synthesis require several steps and complex separation techniques and lead only to low yields. Using the newly discovered unspecific peroxygenase TanUPO from the ascomycetous fungus Truncatella angustata, 90\% regioselective conversion of AA to 14,15-EET could be achieved. Selective conversion of AA to 18-HETE, 19-HETE as well as to 11,12-EET and 14,15-EET was also demonstrated with known peroxygenases, i.e., AaeUPO, CraUPO, MroUPO, MweUPO and CglUPO. The metabolites were confirmed by HPLC-ELSD, MS1 and MS2 spectrometry as well as by comparing their analytical data with authentic standards. Protein structure simulations of TanUPO provided insights into its substrate access channel and give an explanation for the selective oxyfunctionalization of AA. The present study expands the scope of UPOs as they can now be used for selective syntheses of AA metabolites that serve as reference material for diagnostics, for structure-function elucidation as well as for therapeutic and pharmacological purposes}, language = {en} } @misc{KayRegaMorenoetal., author = {Kay, Sonja and Rega, Carlo and Moreno, Gerardo and Herder, Michael den and Palma, Jo{\~a}o H. N. and Borek, Robert and Crous-Duran, Josep and Freese, Dirk and Giannitsopoulos, Michail and Graves, Anil and J{\"a}ger, Mareike and Lamersdorf, Norbert and Memedemin, Daniyar and Mosquera-Losada, R. and Pantera, Anastasia and Paracchini, Maria Luisa and Paris, Pierluigi and Roces-D{\´i}az, Jos{\´e} V. and Rolo, Victor and Rosati, Adolfo and Sandor, Mignon and Smith, Jo and Szerencsits, Erich and Varga, Anna and Viaud, Val{\´e}rie and Wawer, Rafal and Burgess, Paul J. and Herzog, Felix}, title = {Agroforestry creates carbon sinks whilst enhancing the environment in agricultural landscapes in Europe}, series = {Land Use Policy}, volume = {83}, journal = {Land Use Policy}, issn = {0264-8377}, doi = {10.1016/j.landusepol.2019.02.025}, pages = {581 -- 593}, abstract = {Agroforestry, relative to conventional agriculture, contributes significantly to carbon sequestration, increases a range of regulating ecosystem services, and enhances biodiversity. Using a transdisciplinary approach, we combined scientific and technical knowledge to evaluate nine environmental pressures in terms of ecosystem services in European farmland and assessed the carbon storage potential of suitable agroforestry systems, proposed by regional experts. First, regions with potential environmental pressures were identified with respect to soil health (soil erosion by water and wind, low soil organic carbon), water quality (water pollution by nitrates, salinization by irrigation), areas affected by climate change (rising temperature), and by underprovision in biodiversity (pollination and pest control pressures, loss of soil biodiversity). The maps were overlaid to identify areas where several pressures accumulate. In total, 94.4\% of farmlands suffer from at least one environmental pressure, pastures being less affected than arable lands. Regional hotspots were located in north-western France, Denmark, Central Spain, north and south-western Italy, Greece, and eastern Romania. The 10\% of the area with the highest number of accumulated pressures were defined as Priority Areas, where the implementation of agroforestry could be particularly effective. In a second step, European agroforestry experts were asked to propose agroforestry practices suitable for the Priority Areas they were familiar with, and identified 64 different systems covering a wide range of practices. These ranged from hedgerows on field boundaries to fast growing coppices or scattered single tree systems. Third, for each proposed system, the carbon storage potential was assessed based on data from the literature and the results were scaled-up to the Priority Areas. As expected, given the wide range of agroforestry practices identified, the carbon sequestration potentials ranged between 0.09 and 7.29 t C ha -1 a -1 . Implementing agroforestry on the Priority Areas could lead to a sequestration of 2.1 to 63.9 million t C a -1 (7.78 and 234.85 million t CO 2eq a -1 ) depending on the type of agroforestry. This corresponds to between 1.4 and 43.4\% of European agricultural greenhouse gas (GHG) emissions. Moreover, promoting agroforestry in the Priority Areas would contribute to mitigate the environmental pressures identified there. We conclude that the strategic and spatially targeted establishment of agroforestry systems could provide an effective means of meeting EU policy objectives on GHG emissions whilst providing a range of other important benefits.}, language = {en} }