@misc{SchulzKruegerGengeLendleinetal., author = {Schulz, Christian and Kr{\"u}ger-Genge, Anne and Lendlein, Andreas and K{\"u}pper, Jan-Heiner and Jung, Friedrich}, title = {Potential Effects of Nonadherent on Adherent Human Umbilical Venous Endothelial Cells in Cell Culture}, series = {International Journal of Molecular Science}, volume = {22}, journal = {International Journal of Molecular Science}, number = {3}, doi = {10.3390/ijms22031493}, pages = {15}, abstract = {The adherence and shear-resistance of human umbilical venous endothelial cells (HUVEC) on polymers is determined in vitro in order to qualify cardiovascular implant materials. In these tests, variable fractions of HUVEC do not adhere to the material but remain suspended in the culture medium. Nonadherent HUVEC usually stop growing, rapidly lose their viability and can release mediators able to influence the growth and function of the adherent HUVEC. The aim of this study was the investigation of the time dependent behaviour of HUVEC under controlled nonadherent conditions, in order to gain insights into potential influences of these cells on their surrounding environment in particular adherent HUVEC in the context of in vitro biofunctionality assessment of cardiovascular implant materials. Data from adherent or nonadherent HUVEC growing on polystyrene-based cell adhesive tissue culture plates (TCP) or nonadhesive low attachment plates (LAP) allow to calculate the number of mediators released into the culture medium either from adherent or nonadherent cells. Thus, the source of the inflammatory mediators can be identified. For nonadherent HUVEC, a time-dependent aggregation without further proliferation was observed. The rate of apoptotic/dead HUVEC progressively increased over 90\% within two days. Concomitant with distinct blebbing and loss of membrane integrity over time, augmented releases of prostacyclin (PGI2, up to 2.91 ± 0.62 fg/cell) and platelet-derived growth factor BB (PDGF-BB, up to 1.46 ± 0.42 fg/cell) were detected. The study revealed that nonadherent, dying HUVEC released mediators, which can influence the surrounding microenvironment and thereby the results of in vitro biofunctionality assessment of cardiovascular implant materials. Neglecting nonadherent HUVEC bears the risk for under- or overestimation of the materials endothelialization potential, which could lead to the loss of relevant candidates or to uncertainty with regard to their suitability for cardiac applications. One approach to minimize the influence from nonadherent endothelial cells could be their removal shortly after observing initial cell adhesion. However, this would require an individual adaptation of the study design, depending on the properties of the biomaterial used.}, language = {en} } @misc{BiermannSommerauerDieschetal., author = {Biermann, Niklas and Sommerauer, Laura and Diesch, Sophia and Koch, Christoph and Jung, Friedrich and Kehrer, Andreas and Prantl, Lukas and Taeger, Christian D.}, title = {The influence of pulsed electromagnetic field therapy (PEMFT) on cutaneous blood flow in healthy volunteers}, series = {Clinical Hemorheology and Microcirculation}, volume = {76}, journal = {Clinical Hemorheology and Microcirculation}, number = {4}, doi = {10.3233/CH-209224}, pages = {495 -- 501}, abstract = {BACKGROUND: The biophysical interaction induced by low energy pulsed electromagnetic field therapy (PEMFT) on the capillary microcirculation is not well understood. Several studies indicate a significant effect of PEMFT in patients with chronic medical conditions. OBJECTIVE: The aim of this study was to evaluate the influence of PEMFT on skin microcirculation in healthy volunteers. METHODS: 15 healthy participants were included. Nine PEMF treatments were applied over three weeks in an 48-hour interval. The PEMFT system (BEMER) was placed beneath one of the participants' leg with the contralateral side serving as a control. A forty-minute application period was preceded by a 10-minute resting phase. Measuring was done using two Laser Doppler probes (LEA) placed on each anterior lateral thigh. RESULTS: All outcome parameters including flow, mixed venous oxygen saturation and relative venous hemoglobin showed a significant increase during the experiment when compared to the baseline values of the resting phase in both groups (p < 0.01). Comparing both groups, the measurement values during the experiment did not differ (p > 0.05) except for higher flow values in the control group (P = 0.03). Over time, baseline values of both groups showed no significant difference (p > 0.05). CONCLUSION: We found a significant increase of all measurement parameters during the study compared to the baseline values with no difference between the PEMF and control group.}, language = {en} } @misc{AkayBashkatovCoyetal., author = {Akay, {\"O}mer and Bashkatov, Aleksandr and Coy, Emerson and Eckert, Kerstin and Einarsrud, Kristian Etienne and Friedrich, Andreas and Kimmel, Benjamin and Loos, Stefan and Mutschke, Gerd and R{\"o}ntzsch, Lars and Symes, Mark D. and Yang, Xuegeng and Brinkert, Katharina}, title = {Electrolysis in reduced gravitational environments: current research perspectives and future applications}, series = {npj Microgravity}, volume = {8}, journal = {npj Microgravity}, issn = {2373-8065}, doi = {10.1038/s41526-022-00239-y}, abstract = {Electrochemical energy conversion technologies play a crucial role in space missions, for example, in the Environmental Control and Life Support System (ECLSS) on the International Space Station (ISS). They are also vitally important for future long-term space travel for oxygen, fuel and chemical production, where a re-supply of resources from Earth is not possible. Here, we provide an overview of currently existing electrolytic energy conversion technologies for space applications such as proton exchange membrane (PEM) and alkaline electrolyzer systems. We discuss the governing interfacial processes in these devices influenced by reduced gravitation and provide an outlook on future applications of electrolysis systems in, e.g., in-situ resource utilization (ISRU) technologies. A perspective of computational modelling to predict the impact of the reduced gravitational environment on governing electrochemical processes is also discussed and experimental suggestions to better understand efficiency-impacting processes such as gas bubble formation and detachment in reduced gravitational environments are outlined.}, language = {en} } @misc{TartivelBlockiBrauneetal., author = {Tartivel, Lucile and Blocki, Anna M. and Braune, Steffen and Jung, Friedrich and Behl, Marc and Lendlein, Andreas}, title = {An Inverse Shape-Memory Hydrogel Scaffold Switching Upon Cooling in a Tissue-Tolerated Temperature Range}, series = {Advanced materials interfaces}, volume = {9}, journal = {Advanced materials interfaces}, number = {6}, issn = {2196-7350}, doi = {10.1002/admi.202101588}, language = {en} } @misc{LauGossenLendleinetal., author = {Lau, S. and Gossen, Maike and Lendlein, Andreas and Jung, Friedrich}, title = {Differential sensitivity of assays for determining vein endothelial cell senescence}, series = {Clinical Hemorheology and Microcirculation}, volume = {81}, journal = {Clinical Hemorheology and Microcirculation}, number = {3}, issn = {1875-8622}, doi = {10.3233/CH-211294}, pages = {191 -- 203}, language = {en} } @misc{EisenhauerFrankWeigeltetal., author = {Eisenhauer, Nico and Frank, Karin and Weigelt, Alexandra and Bartkowski, Bartosz and Beugnon, R{\´e}my and Liebal, Katja and Mahecha, Miguel and Quaas, Martin and Al-Halbouni, Djamil and Bastos, Ana and Bohn, Friedrich J. and Brito, Mariana Madruga de and Denzler, Joachim and Feilhauer, Hannes and Fischer, Rico and Fritsche, Immo and Guimaraes-Steinicke, Claudia and H{\"a}nsel, Martin and Haun, Daniel B. M. and Herrmann, Hartmut and Huth, Andreas and Kalesse-Los, Heike and Koetter, Michael and Kolleck, Nina and Krause, Melanie and Kretschmer, Marlene and Leit{\~a}o, Pedro J. and Masson, Torsten and Mora, Karin and M{\"u}ller, Birgit and Peng, Jian and P{\"o}hlker, Mira L. and Ratzke, Leonie and Reichstein, Markus and Richter, Solveig and R{\"u}ger, Nadja and S{\´a}nchez-Parra, Beatriz and Shadaydeh, Maha and Sippel, Sebastian and Tegen, Ina and Thr{\"a}n, Daniela and Umlauft, Josefine and Wendisch, Manfred and Wolf, Kevin and Wirth, Christian and Zacher, Hannes and Zaehle, S{\"o}nke and Quaas, Johannes}, title = {A belowground perspective on the nexus between biodiversity change, climate change, and human well-being}, series = {Journal of Sustainable Agriculture and Environment}, volume = {3}, journal = {Journal of Sustainable Agriculture and Environment}, number = {2}, publisher = {Wiley}, issn = {2767-035X}, doi = {10.1002/sae2.12108}, pages = {12}, abstract = {Soil is central to the complex interplay among biodiversity, climate, and society. This paper examines the interconnectedness of soil biodiversity, climate change, and societal impacts, emphasizing the urgent need for integrated solutions. Human-induced biodiversity loss and climate change intensify environmental degradation, threatening human well-being. Soils, rich in biodiversity and vital for ecosystem function regulation, are highly vulnerable to these pressures, affecting nutrient cycling, soil fertility, and resilience. Soil also crucially regulates climate, influencing energy, water cycles, and carbon storage. Yet, climate change poses significant challenges to soil health and carbon dynamics, amplifying global warming. Integrated approaches are essential, including sustainable land management, policy interventions, technological innovations, and societal engagement. Practices like agroforestry and organic farming improve soil health and mitigate climate impacts. Effective policies and governance are crucial for promoting sustainable practices and soil conservation. Recent technologies aid in monitoring soil biodiversity and implementing sustainable land management. Societal engagement, through education and collective action, is vital for environmental stewardship. By prioritizing interdisciplinary research and addressing key frontiers, scientists can advance understanding of the soil biodiversity-climate change-society nexus, informing strategies for environmental sustainability and social equity.}, language = {en} }