@misc{BremenkampMeissnerCommichau, author = {Bremenkamp, Rica and Meißner, Janek and Commichau, Fabian M.}, title = {Ein bakterielles System zum Nachweis und Abbau des Totalherbizids Glyphosat}, series = {Biologie in unserer Zeit}, volume = {50}, journal = {Biologie in unserer Zeit}, number = {1}, issn = {1521-415X}, doi = {10.1002/biuz.202070107}, pages = {15 -- 17}, abstract = {Jedes Jahr aufs Neue kommen Studenten aus aller Welt zusammen und messen sich im internationalen Wettbewerb iGEM auf dem Gebiet der synthetischen Biologie. Dieses Jahr hat das iGEM-Team aus G{\"o}ttingen (Abbildung 1) sich mit dem kontrovers diskutierten Totalherbizid Glyphosat befasst und ein bakterielles System zum Nachweis und Abbau des Herbizids entwickelt.}, language = {de} } @misc{TaeuberDormeyerCommichauetal., author = {T{\"a}uber, Sarah and Dormeyer, Miriam and Commichau, Fabian M. and Gr{\"u}nberger, Alexander}, title = {Visualisierung von Mutationen auf Einzelzellebene}, series = {BioSpektrum}, volume = {26}, journal = {BioSpektrum}, number = {4}, issn = {1868-6249}, doi = {10.1007/s12268-020-1414-2}, pages = {388 -- 390}, abstract = {Bacterial mutations have been investigated since many years, but they remain difficult to observe directly in single cells, which limits the analysis of the underlying molecular mechanism. However, for the investigation of mutations at the level of single cells, precise analytical tools are currently developed. This article describes a workflow for visualizing mutations in single cells and lays the foundation for the quantification of bacterial mutation rates in the future.}, language = {de} } @misc{Commichau, author = {Commichau, Fabian M.}, title = {Hochvirulente Isolate des pathogenen Bakteriums Listeria monocytogenes}, series = {BioSpektrum}, volume = {26}, journal = {BioSpektrum}, number = {3}, issn = {1868-6249}, doi = {10.1007/s12268-020-1383-5}, pages = {S. 280}, abstract = {Das Gram-positive Bakterium Listeria monocytogenes kommt sowohl freilebend als auch als Pathogen in verschiedenen Wirbeltiertaxa sowie in Crustaceen vor. Gelangt L. monocytogenes {\"u}ber die Nah- rung in den menschlichen K{\"o}rper, kann es Listeriose ausl{\"o}sen. Diese bakterielle Infektion ist f{\"u}r immunsupprimierte und {\"a}ltere Menschen sowie f{\"u}r schwangere Frauen besonders gef{\"a}hrlich. Aufgrund unzureichender hygienischer Maßnahmen bei der Lebensmittelzubereitung kam es in der Vergangenheit immer wieder zu Todesf{\"a}llen. K{\"u}rzlich berichteten Yuelan Yin et al. (Nat Commun (2019) 10:4283) von den virulentesten L. monocytogenes- Isolaten.}, language = {de} } @misc{KohmFloccariLutzetal., author = {Kohm, Katharina and Floccari, Valentina and Lutz, Veronika and Nordmann, Birthe and Mittelst{\"a}dt, Carolin and Poehlein, Anja and Dragos, Anna and Commichau, Fabian M. and Hertel, Robert}, title = {The Bacillus phage SPβ and its relatives: A temperate phage model system reveals new strains, species, prophage integration loci, conserved proteins and lysogeny management components}, series = {bioRxiv beta}, journal = {bioRxiv beta}, doi = {10.1101/2021.11.22.469490}, pages = {44}, abstract = {The Bacillus phage SPβ has been known for about 50 years, but only a few strains are avalible. We isolated four new wild type strains of the SPbeta species. Phage vB_BsuS-Goe14 introduces its prophage into the spoVK locus, previously not observed to be used by SPβ-like phages. We could also reveal the SPβ-like phage genome replication strategy, the genome packaging mode, and the phage genome opening point. We extracted 55 SPβ-like prophages from public Bacillus genomes, thereby discovering three more integration loci and one additional type of integrase. The identified prophages resembled four new species clusters and three species orphans in the genus Spbetavirus. The determined core proteome of all SPβ-like prophages consists of 38 proteins. The integration cassette proved to be not conserved even though present in all strains. It consists of distinct integrases. Analysis of SPβ transcriptomes revealed three conserved genes, yopQ, yopR, and yokI, to be transcribed from a dormant prophage. While yopQ and yokI could be deleted from the prophage without activating the prophage, damaging of yopR led to a clear-plaque phenotype. Under the applied laboratory conditions, the yokI mutant showed an elevated virion release implying the YokI protein being a component of the arbitrium system.}, language = {en} } @misc{RiedelCommichauBenndorfetal., author = {Riedel, Ramona and Commichau, Fabian M. and Benndorf, Dirk and Martienssen, Marion}, title = {Biologischer Abbau von Aminophosphonaten}, series = {BIOspektrum : Das Magazin f{\"u}r Biowissenschaften}, volume = {30}, journal = {BIOspektrum : Das Magazin f{\"u}r Biowissenschaften}, issn = {1868-6249}, doi = {10.1007/s12268-024-2179-9}, pages = {348 -- 350}, abstract = {Application of commercial aminophosphonates such as glyphosate (GS) or chelating agents in detergent are daily used at large scale worldwide. Only little is known about the potential biodegradation of the latter. Here we describe the characterization of the new strain Ochrobactrum sp. BTU1 isolated from a LC/MS standard solution. The strain is capable to degrade all investigated aminophosphonates including GS.}, language = {de} }