@misc{LutterLiedtkeEckeetal., author = {Lutter, Anne-Helen and Liedtke, Victoria and Ecke, Annemarie and Scholka, Jenny and Muschter, Antje and Hentschel, Erik and Becker, Roland and Anderer, Ursula}, title = {Direct co-culture of human chondrogenic microtissues with osteoblast-like Saos-2 cells positively influence the differentiation potential of cartilage cells in 3D culture}, doi = {10.1007\%2Fs10354-018-0664-9.pdf}, pages = {S. 51}, language = {en} } @misc{EckeScholkaHanschetal., author = {Ecke, Annemarie and Scholka, Jenny and Hansch, Anna and Becker, Roland and Anderer, Ursula}, title = {Agar overlay technique vs. v-bottom plate: differentiation of human cartilage-like microtissues depend on plate format.}, series = {Osteologie}, volume = {28}, journal = {Osteologie}, number = {4}, publisher = {Georg Thieme Verlag KG}, address = {Stuttgart ; New York}, issn = {2567-5818}, doi = {10.1055/s-0039-1680058}, pages = {81}, language = {en} } @misc{LutterEckeScholkaetal., author = {Lutter, Anne-Helen and Ecke, Annemarie and Scholka, Jenny and Waldeck, Peter and Tretter, Tim and Anderer, Ursula}, title = {Differentiation of human chondrocytes under the influence of microalgae-based exopolysaccharides}, series = {38. Jahrestagung der Deutschen Gesellschaft f{\"u}r Klinische Mikrozirkulation und H{\"a}morheologie : Programm, 22. und 23. November 2019, Braunschweig}, journal = {38. Jahrestagung der Deutschen Gesellschaft f{\"u}r Klinische Mikrozirkulation und H{\"a}morheologie : Programm, 22. und 23. November 2019, Braunschweig}, abstract = {BACKGROUND: Exopolysaccharides are formed by microalgae in response to adverse conditions and are found mainly as a coating around the cells. The protective function of EPS plays an important role in the survival of microalgae in polluted habitats. So far, the particular biological and physico-chemical properties of EPS have not been adequately investigated and their effect on cells such as human chondrocytes has not been tested. Microalgae in particular offer an ideal platform for the production of dietary supplements or drugs that enable new therapeutic approaches. METHODS: To investigate the influence of EPS on human chondrocytes, cytotoxicity and differentiation potential were analyzed in 3D cultures. RESULTS: We were able to demonstrate a positive, but donor-specific influence of EPS on the differentiation of human chondrocytes. Furthermore, the EPS showed no cytotoxic effects in a range of up to 250 µg EPS per ml medium. CONCLUSION: EPS have the potential to positively influence the differentiation of human chondrocytes. Further experiments are necessary to analyze the donor-specific effect.}, language = {en} } @misc{EckeScholkaHanschetal., author = {Ecke, Annemarie and Scholka, Jenny and Hansch, Anna and Becker, Roland and Anderer, Ursula}, title = {Differentiation of human articular chondrocytes in microtissues depends on the cell aggregation technique}, pages = {1}, language = {en} } @misc{AndererScholkaHanschetal., author = {Anderer, Ursula and Scholka, Jenny and Hansch, Anna and Becker, Roland and Ecke, Annemarie}, title = {Influence of the cell aggregation technique on the differentiation of human articular chondrocytes in microtissues}, series = {Osteologie / Osteology}, volume = {31}, journal = {Osteologie / Osteology}, number = {03}, publisher = {Georg Thieme Verlag}, address = {Stuttgart}, issn = {1019-1291}, doi = {10.1055/s-0042-1755855}, pages = {201 -- 202}, language = {en} } @misc{EckeLutterScholkaetal., author = {Ecke, Annemarie and Lutter, Anne-Helen and Scholka, Jenny and Hansch, Anna and Becker, Roland and Anderer, Ursula}, title = {Tissue Specific Differentiation of Human Chondrocytes Depends on Cell Microenvironment and Serum Selection}, series = {Cells}, volume = {8}, journal = {Cells}, number = {8}, issn = {2073-4409}, doi = {10.3390/cells8080934}, pages = {15}, language = {en} } @misc{EckeScholkaHanschetal., author = {Ecke, Annemarie and Scholka, Jenny and Hansch, Anna and Becker, Roland and Anderer, Ursula}, title = {Human serum or FCS: Differentiation of human cartilage-like microtissues depends on serum selection}, series = {DGZ 2018 International Meeting of the German Society for Cell Biology: "Concepts of Cell Organisation and Dynamics"}, journal = {DGZ 2018 International Meeting of the German Society for Cell Biology: "Concepts of Cell Organisation and Dynamics"}, abstract = {Introduction: Regeneration of articular cartilage defects is limited. Hence, cell-based therapies for the treatment were developed using e.g. chondrocytes isolated from patient biopsies. For cell culture fetal calf serum (FCS) is often used as media supplement to establish engineering processes for transplants in regenerative medicine. However, the usage of xenogeneic serum could mask the intrinsic behavior of human cells in vitro. This could be particularly important for the dedifferentiation process during cell expansion and redifferentiation in 3D-culture. The aim of this study was to compare the development of microtissues derived from chondrocytes cultivated in presence of FCS with those cultivated in human serum (HS). Material and Methods: Human articular chondrocytes isolated from knee joints were expanded in medium with HS or FCS. Scaffold-free microtissues were generated using the agar overlay technique. After four and eight weeks the macroscopic appearance (reflected-light microscopy) was documented and the size of the microtissues was determined. The differentiation degree was evaluated via histology to visualize typical glycosamino¬glycans (Safranin O, Alcian blue) and immunohisto¬chemistry (IH) to detect cartilage-specific markers (collagen type II, proteoglycans, COMP, Sox9) on cryosections. Results and Conclusion: Cells from all donors in both medium compositions generated microtissues - but with distinct differences in the differentiation levels. Microtissues cultured in medium with FCS showed a higher differentiation level compared to microtissues in HS. This is evidenced by a higher amount of matrix resulting in bigger microtissues (diameter in FCS is 42\% larger compared to HS) and a more cartilage-like composition of the matrix (not any/little colouring in HS versus intense staining in FCS). IH analysis showed the same pattern. Not any/less positivity for collagen type II, proteoglycan, COMP, and Sox9 in HS versus moderate-to-high positivity in FCS-cultured microtissues. Absolute values vary from donor to donor whereas an individualized expression profile only became visible in microtissues cultured in HS medium. HS should be preferred for the engineering of cartilage-like microtissues, as it rather enables a "human-based" situation in vitro. Supported by „Gesundheitscampus Brandenburg" and „Ministerium f{\"u}r Wissenschaft, Forschung und Kultur", state of Brandenburg, Germany.}, language = {en} }