@misc{FroemmelLehmannRoedigeretal., author = {Fr{\"o}mmel, Ulrike and Lehmann, Werner and R{\"o}diger, Stefan and B{\"o}hm, Alexander and Nitschke, J{\"o}rg and Weinreich, J{\"o}rg and Groß, Julia and Roggenbuck, Dirk and Zinke, Olaf and Ansorge, Hermann and Vogel, Steffen and Klemm, Per and Wex, Thomas and Schr{\"o}der, Christian and Wieler, Lothar H. and Schierack, Peter}, title = {Adhesion of human and animal Escherichia coli strains in association with their virulence-associated genes and phylogenetic origins}, series = {Applied and environmental microbiology}, volume = {79}, journal = {Applied and environmental microbiology}, number = {19}, issn = {1098-5336}, doi = {10.1128/AEM.01384-13}, pages = {5814 -- 5829}, abstract = {Intestinal colonization is influenced by the ability of the bacterium to inhabit a niche, which is based on the expression of colonization factors. Escherichia coli carries a broad range of virulence-associated genes (VAGs) which contribute to intestinal (inVAGs) and extraintestinal (exVAGs) infection. Moreover, initial evidence indicates that inVAGs and exVAGs support intestinal colonization. We developed new screening tools to genotypically and phenotypically characterize E. coli isolates originating in humans, domestic pigs, and 17 wild mammal and avian species. We analyzed 317 isolates for the occurrence of 44 VAGs using a novel multiplex PCR microbead assay (MPMA) and for adhesion to four epithelial cell lines using a new adhesion assay. We correlated data for the definition of new adhesion genes. inVAGs were identified only sporadically, particularly in roe deer (Capreolus capreolus) and the European hedgehog ( Erinaceus europaeus). The prevalence of exVAGs depended on isolation from a specific host. Human uropathogenic E. coli isolates carried exVAGs with the highest prevalence, followed by badger (Meles meles) and roe deer isolates. Adhesion was found to be very diverse. Adhesion was specific to cells, host, and tissue, though it was also unspecific. Occurrence of the following VAGs was associated with a higher rate of adhesion to one or more cell lines: afa-dra, daaD, tsh, vat, ibeA, fyuA, mat, sfa-foc, malX, pic, irp2, and papC. In summary, we established new screening methods which enabled us to characterize large numbers of E. coli isolates. We defined reservoirs for potential pathogenic E. coli. We also identified a very broad range of colonization strategies and defined potential new adhesion genes.}, language = {en} } @misc{FroemmelBoehmNitschkeetal., author = {Fr{\"o}mmel, Ulrike and B{\"o}hm, Alexander and Nitschke, J{\"o}rg and Weinreich, J{\"o}rg and Groß, Julia and R{\"o}diger, Stefan and Wex, Thomas and Ansorge, Hermann and Zinke, Olaf and Schr{\"o}der, Christian and Roggenbuck, Dirk and Schierack, Peter}, title = {Adhesion patterns of commensal and pathogenic Escherichia coli from humans and wild animals on human and porcine epithelial cell lines}, series = {Gut Pathogens}, volume = {5}, journal = {Gut Pathogens}, number = {31}, issn = {1757-4749}, doi = {10.1186/1757-4749-5-31}, pages = {8}, abstract = {Abstract BACKGROUND: Different strategies of colonization or infection by E. coli result in formation of certain adhesion patterns which help also in classifying intestinal E. coli into pathotypes. Little is known about adhesion patterns and host- and tissue adaption of commensal E. coli and about E. coli originating in clinically healthy hosts carrying pathotype-specific virulence-associated genes. FINDINGS: Adhesion pattern of E. coli (n = 282) from humans and from 18 animal species were verified on intestinal human Caco-2 and porcine IPEC-J2 cells and, furthermore, for comparison on human urinary bladder 5637, porcine kidney PK-15 epithelial and HEp-2 cells. The analysis was carried out on 150,000 images of adhesion assays.Adhesion patterns were very diverse; 88 isolates were completely non-adherent, whereas 194 adhered to at least one cell line with the dominant adhesion patterns "diffusely distributed" and "microcolony formation". Adhesion patterns "chains" and "clumps" were also visible. Chain formation was mediated by the presence of epithelial cells. Clump formation was very specific on only the 5637 cell line. All enteropathogenic (eae+) E. coli (EPEC; n = 14) were able to form microcolonies which was cell line specific for each isolate. Most EPEC formed microcolonies on intestinal IPEC-J2 and Caco-2 but several also on urinary tract cells. Shigatoxin-producing (stx+) E. coli (n = 10) showed no specific adhesion patterns. CONCLUSIONS: E. coli isolates were highly diverse. Commensal and pathogenic isolates can adhere in various forms, including diffuse distribution, microcolonies, chains and clumps. Microcolony formation seems to be a global adhesion strategy also for commensal E. coli.}, language = {en} } @misc{GrossmannRoggenbuckSchroederetal., author = {Großmann, Kai and Roggenbuck, Dirk and Schr{\"o}der, Christian and Conrad, Karsten and Schierack, Peter and Sack, Ulrich}, title = {Multiplex assessment of non-organ-specific autoantibodies with a novel microbead-based immunoassay}, series = {Cytometry Part A}, volume = {79}, journal = {Cytometry Part A}, number = {2}, issn = {1552-4922}, doi = {10.1002/cyto.a.21009}, pages = {118 -- 125}, language = {en} } @incollection{RoedigerSchierackBoehmetal., author = {R{\"o}diger, Stefan and Schierack, Peter and B{\"o}hm, Alexander and Nitschke, J{\"o}rg and Berger, Ingo and Fr{\"o}mmel, Ulrike and Schmidt, Carsten and Ruhland, Mirko and Schimke, Ingolf and Roggenbuck, Dirk and Lehmann, Werner and Schr{\"o}der, Christian}, title = {A highly versatile microscope imaging technology platform for the multiplex real-time detection of biomolecules and autoimmune antibodies}, series = {Molecular diagnostics}, booktitle = {Molecular diagnostics}, editor = {Seitz, Harald and Berger, Ingo}, publisher = {Springer}, address = {Berlin [u.a.]}, isbn = {978-3-642-37690-0}, pages = {35 -- 74}, language = {en} } @misc{NalliSommaAndreolietal., author = {Nalli, Cecilia and Somma, Valentina and Andreoli, Laura and B{\"u}ttner, Thomas and Schierack, Peter and Mahler, Michael and Roggenbuck, Dirk and Tincani, Angela}, title = {Anti-phospholipid IgG antibodies detected by line immunoassay differentiate patients with anti-phospholipid syndrome and other autoimmune diseases}, series = {Autoimmunity Highlights}, volume = {9}, journal = {Autoimmunity Highlights}, number = {1}, issn = {2038-3274}, doi = {10.1007/s13317-018-0106-0}, pages = {11}, language = {en} } @misc{KolendaBurdukiewiczSchiebeletal., author = {Kolenda, Rafał and Burdukiewicz, Michał and Schiebel, Juliane and R{\"o}diger, Stefan and Sauer, Lysann and Szabo, Istvan and Orlowska, Aleksandra and Weinreich, J{\"o}rg and Nitschke, J{\"o}rg and B{\"o}hm, Alexander and Gerber, Ulrike and Roggenbuck, Dirk and Schierack, Peter}, title = {Adhesion of Salmonella to Pancreatic Secretory Granule Membrane Major Glycoprotein GP2 of Human and Porcine Origin Depends on FimH Sequence Variation}, series = {Frontiers in Microbiology}, volume = {9}, journal = {Frontiers in Microbiology}, number = {1905}, issn = {1664-302X}, doi = {10.3389/fmicb.2018.01905}, pages = {15}, language = {en} } @misc{SowaKolendaBaumgartetal., author = {Sowa, Mandy and Kolenda, Rafał and Baumgart, Daniel C. and Pratschke, Johann and Papp, Maria and Tornai, Tamas and Suchanski, Jaroslaw and Bogdanos, Dimitrios Petrou and Mytilinaiou, Maria G. and Hammermann, Jutta and Laass, Martin W. and Conrad, Karsten and Schramm, Christoph and Franke, Andre and Roggenbuck, Dirk and Schierack, Peter}, title = {Mucosal Autoimmunity to Cell-Bound GP2 Isoforms Is a Sensitive Marker in PSC and Associated With the Clinical Phenotype}, series = {Frontiers in Immunology}, volume = {9}, journal = {Frontiers in Immunology}, number = {1959}, issn = {1664-3224}, doi = {10.3389/fimmu.2018.01959}, language = {en} } @misc{MaslyanskiyLazarevaOlineketal., author = {Maslyanskiy, Aleksey and Lazareva, Natalya and Olinek, Polina and Schierack, Peter and Hentschel, Christian and Cuccato, Juliane and Bogdanos, Dimitrios Petrou and Lapin, Sergey V. and Roggenbuck, Dirk}, title = {Anti-hnRNP B1 (RA33) Autoantibodies Are Associated with the Clinical Phenotype in Russian Patients with Rheumatoid Arthritis and Systemic Sclerosis}, series = {Journal of Immunology Research}, volume = {2014}, journal = {Journal of Immunology Research}, doi = {10.1155/2014/516593}, pages = {7}, language = {en} } @misc{MirskyRoedigerSchieracketal., author = {Mirsky, Vladimir M. and R{\"o}diger, Stefan and Schierack, Peter and Roggenbuck, Dirk}, title = {Preface to the Special Issue on PCR on chip and related technologies}, series = {Microchimica Acta}, volume = {181}, journal = {Microchimica Acta}, number = {13/14}, doi = {10.1007/s00604-014-1246-1}, pages = {1609 -- 1610}, language = {en} } @misc{SowaGrossmannScholzetal., author = {Sowa, Mandy and Großmann, Kai and Scholz, Juliane and R{\"o}ber, Nadja and R{\"o}diger, Stefan and Schierack, Peter and Conrad, Karsten and Roggenbuck, Dirk and Hiemann, Rico}, title = {Der CytoBead-Assay - Eine neue M{\"o}glichkeit der multiparametrischen Autoantik{\"o}rperanalytik bei systemischen Autoimmunerkrankungen}, series = {Journal of Laboratory Medicine}, volume = {38}, journal = {Journal of Laboratory Medicine}, number = {6}, doi = {10.1515/labmed-2014-0041}, pages = {309 -- 317}, language = {de} } @misc{RoggenbuckSommaSchieracketal., author = {Roggenbuck, Dirk and Somma, Valentina and Schierack, Peter and Borghi, Maria Orietta and Meroni, Pier Luigi}, title = {Autoantibody profiling in APS}, series = {Lupus}, volume = {23}, journal = {Lupus}, number = {12}, issn = {0961-2033}, doi = {10.1177/0961203314534305}, pages = {1262 -- 1264}, language = {en} } @misc{RoggenbuckBorghiSommaetal., author = {Roggenbuck, Dirk and Borghi, Maria Orietta and Somma, Valentina and B{\"u}ttner, Thomas and Schierack, Peter and Hanack, Katja and Grossi, Claudia and Bodio, Caterina and Macor, Paolo and Landenberg, Philipp von and Boccellato, Francesco and Mahler, Michael and Meroni, Pier Luigi}, title = {Antiphospholipid antibodies detected by line immunoassay differentiate among patients with antiphospholipid syndrome, with infections and asymptomatic carriers}, series = {Arthritis Research \& Therapy}, volume = {18}, journal = {Arthritis Research \& Therapy}, number = {111}, issn = {1465-9913}, doi = {10.1186/s13075-016-1018-x}, pages = {14}, language = {en} } @misc{SowaTrezziHiemannetal., author = {Sowa, Mandy and Trezzi, Barbara and Hiemann, Rico and Schierack, Peter and Großmann, Kai and Scholz, Juliane and Somma, Valentina and Sinico, Renato Alberto and Roggenbuck, Dirk and Radice, Antonella}, title = {Simultaneous comprehensive multiplex autoantibody analysis for rapidly progressive glomerulonephritis}, series = {Medicine}, volume = {95}, journal = {Medicine}, number = {44}, issn = {0025-7974}, doi = {10.1097/MD.0000000000005225}, pages = {5225}, language = {en} } @misc{RoedigerKramerFroemmeletal., author = {R{\"o}diger, Stefan and Kramer, Toni and Fr{\"o}mmel, Ulrike and Weinreich, J{\"o}rg and Roggenbuck, Dirk and Guenther, Sebastian and Schaufler, Katharina and Schr{\"o}der, Christian and Schierack, Peter}, title = {Intestinal Escherichia coli colonization in a mallard duck population over four consecutive winter season}, series = {Environmental Microbiology}, volume = {17}, journal = {Environmental Microbiology}, number = {9}, issn = {1462-2920}, doi = {10.1111/1462-2920.12807}, pages = {3352 -- 3361}, language = {en} } @misc{VillaltaMytilinaiouElsneretal., author = {Villalta, Danilo and Mytilinaiou, Maria G. and Elsner, Martina and Hentschel, Christian and Cuccato, Juliane and Somma, Valentina and Schierack, Peter and Roggenbuck, Dirk and Bogdanos, Dimitrios Petrou}, title = {Autoantibodies to asialoglycoprotein receptor (ASGPR) in patients with autoimmune liver diseases}, series = {Clinica Chimica Acta}, volume = {450}, journal = {Clinica Chimica Acta}, issn = {0009-8981}, doi = {10.1016/j.cca.2015.07.021}, pages = {5}, language = {en} } @inproceedings{RoedigerBoehmNitschkeetal., author = {R{\"o}diger, Stefan and B{\"o}hm, Alexander and Nitschke, J{\"o}rg and Stavitskaya, Luba and Gruner, Melanie and Kundzer, Alena V. and Volkova, Margarita V. and Generalov, I. and Schmidt, Carsten and Schr{\"o}der, Christian and Roggenbuck, Dirk and Schierack, Peter}, title = {Development of a Method for Multiplex Real-Time Analysis of Enzymatic Activity on a Microbead-Chip}, series = {Infection, tumors and autoimmunity, report on the 11th Dresden Symposium on Autoantibodies held in Dresden on September 1-4, 2013}, booktitle = {Infection, tumors and autoimmunity, report on the 11th Dresden Symposium on Autoantibodies held in Dresden on September 1-4, 2013}, publisher = {Pabst Science Publ.}, address = {Lengerich [u.a.]}, isbn = {978-3-89967-881-9}, pages = {275 -- 280}, language = {en} } @inproceedings{RoedigerBurdukiewiczHeiserichetal., author = {R{\"o}diger, Stefan and Burdukiewicz, Michał and Heiserich, Lisa and Schierack, Peter and Roggenbuck, Dirk}, title = {Digital Enumeration of Double Strand Breaks via γH2AX and Aassociated biomarkers in a Computing Environment for Reproducible Research}, series = {From autoantibody research to standardized diagnostic assays in the management of human diseases, report on the 12th Dresden Symposium on Autoantibodies, September 23-26, 2015}, booktitle = {From autoantibody research to standardized diagnostic assays in the management of human diseases, report on the 12th Dresden Symposium on Autoantibodies, September 23-26, 2015}, publisher = {Pabst Science Publishers}, address = {Lengerich}, isbn = {978-3-95853-104-8}, pages = {S. 413}, language = {en} } @misc{RoedigerSchneiderSaueretal., author = {R{\"o}diger, Stefan and Schneider, Jens and Sauer, Lysann and Reddig, Annika and Schierack, Peter and Roggenbuck, Dirk and Schr{\"o}der, Christian}, title = {A Multiparameter Platform for The Analysis of DNA Double-strand Breaks and Associated Biomarkers after genome Editing Austrian Biomarker Symposium (ASBD) 2016, 10.-11.03.2016, Wien, {\"O}sterreich}, language = {en} } @misc{SauerRoedigerSchneideretal., author = {Sauer, Lysann and R{\"o}diger, Stefan and Schneider, Jens and Schierack, Peter and Roggenbuck, Dirk and Schr{\"o}der, Christian}, title = {Functional Multiparameter Analy sis of Double-Strand Breaks and Associate Biomarkers during Genome Editing, Genome Editing and Gene Modulation Congress 2016, 6.-8.04.2016, Oxford, United Kingdom}, language = {en} } @misc{DeutschmannDinterRoedigeretal., author = {Deutschmann, Claudia and Dinter, Franziska and R{\"o}diger, Stefan and Roggenbuck, Dirk and Schierack, Peter}, title = {Comparison of Lab and Point of Care (POC) technologies - case study for CHI3L1, Potsdam Days on Bioanalysis}, pages = {1}, language = {en} } @inproceedings{RoedigerBoehmNitschkeetal., author = {R{\"o}diger, Stefan and B{\"o}hm, Alexander and Nitschke, J{\"o}rg and Stavitskaya, Luba and Gruner, Melanie and Kundzer, Alena V. and Volkova, Margarita V. and Generalov, I. and Schmidt, Carsten and Schr{\"o}der, Christian and Roggenbuck, Dirk and Schierack, Peter}, title = {Development of a Method for Multiplex Real-Time Analysis of Enzymati c Activity on a Microbead-Chip.}, series = {Infections, Tumors and Autoimmunity, Report on the 11th Dresden Symposium on Autoantibodies held in Dresden on September 01. - 04., 2013}, booktitle = {Infections, Tumors and Autoimmunity, Report on the 11th Dresden Symposium on Autoantibodies held in Dresden on September 01. - 04., 2013}, publisher = {Pabst Science Publ.}, address = {Lengerich}, isbn = {978-3-89967-881-9}, pages = {280}, language = {en} } @misc{SchiebelBoehmNitschkeetal., author = {Schiebel, Juliane and B{\"o}hm, Alexander and Nitschke, J{\"o}rg and Burdukiewicz, Michał and Weinreich, J{\"o}rg and Ali, Aamir and Roggenbuck, Dirk and R{\"o}diger, Stefan and Schierack, Peter}, title = {Genotypic and phenotypic characteristics in association with biofilm formation in different pathotypes of human clinical Escherichia coli isolates}, series = {Applied and Environmental Microbiology}, volume = {83}, journal = {Applied and Environmental Microbiology}, number = {24}, issn = {1098-5336}, doi = {10.1128/AEM.01660-17}, language = {en} } @misc{LiebschRoedigerBoehmetal., author = {Liebsch, Claudia and R{\"o}diger, Stefan and B{\"o}hm, Alexander and Nitschke, J{\"o}rg and Weinreich, J{\"o}rg and Fruth, Angelika and Roggenbuck, Dirk and Lehmann, Werner and Schedler, Uwe and Juretzek, Thomas and Schierack, Peter}, title = {Solid-phase microbead array for multiplex O-serotyping of Escherichia coli}, series = {Microchimica Acta}, volume = {184}, journal = {Microchimica Acta}, number = {5}, issn = {0026-3672}, doi = {10.1007/s00604-017-2088-4}, pages = {1405 -- 1415}, language = {en} } @misc{DeutschmannRoggenbuckSchierack, author = {Deutschmann, Claudia and Roggenbuck, Dirk and Schierack, Peter}, title = {The loss of tolerance to CHI3L1-A putative role in inflammatory bowel disease?}, series = {Clinical Immunology}, volume = {199}, journal = {Clinical Immunology}, issn = {1521-6616}, doi = {10/gftf2k}, pages = {12 -- 17}, language = {en} } @misc{SchneiderWeissRuheetal., author = {Schneider, Jens and Weiss, Romano and Ruhe, Madeleine and Jung, Tobias and Roggenbuck, Dirk and Stohwasser, Ralf and Schierack, Peter and R{\"o}diger, Stefan}, title = {Open source bioimage informatic tools for the analysis of DNA damage and associated biomarkers}, series = {Journal of Laboratory and Precision Medicine}, volume = {4}, journal = {Journal of Laboratory and Precision Medicine}, issn = {2519-9005}, doi = {10.21037/jlpm.2019.04.05}, language = {en} } @misc{ThalerBietenbeckSteigerwaldetal., author = {Thaler, Markus A. and Bietenbeck, Andreas and Steigerwald, Udo and B{\"u}ttner, Thomas and Schierack, Peter and Lindhoff-Last, Edelgard and Roggenbuck, Dirk and Luppa, Peter B.}, title = {Evaluation of the sensitivityficity of a novel line immunoassay for the detection of criteria and non-criteria antiphospholipid antibodies in comparison to establishedELISAs}, series = {PLoS One}, volume = {14}, journal = {PLoS One}, number = {7}, issn = {1932-6203}, doi = {10.1371/journal.pone.0220033}, pages = {17}, language = {en} } @misc{DeutschmannRoggenbuckSchierack, author = {Deutschmann, Claudia and Roggenbuck, Dirk and Schierack, Peter}, title = {The loss of tolerance to CHI3L1-A putative role in inflammatory bowel disease?}, series = {Clinical Immunology}, volume = {199}, journal = {Clinical Immunology}, issn = {1521-6616}, doi = {10.1016/j.clim.2018.12.005}, pages = {12 -- 17}, language = {en} } @misc{DeutschmannSowaMurugaiyanetal., author = {Deutschmann, Claudia and Sowa, Mandy and Murugaiyan, Jayaseelan and Roessler, Uwe and R{\"o}ber, Nadja and Conrad, Karsten and Laass, Martin W. and Bogdanos, Dimitrios Petrou and Sipeki, Nora and Papp, Maria and R{\"o}diger, Stefan and Roggenbuck, Dirk and Schierack, Peter}, title = {Identification of Chitinase-3-Like Protein 1 as a Novel Neutrophil Antigenic Target in Crohn's Disease}, series = {Journal of Crohn's and Colitis}, volume = {13}, journal = {Journal of Crohn's and Colitis}, number = {7}, issn = {1876-4479}, doi = {10.1093/ecco-jcc/jjz012}, pages = {894 -- 904}, abstract = {Background and Aims There is an increasing incidence of inflammatory bowel disease [IBD]. Autoimmune responses are involved in the pathophysiology of IBD, but their underlying pathways and target antigens have not yet been fully elucidated. Methods Autoantigenic targets in IBD were identified after separation of whole cell proteins isolated from neutrophils using two-dimensional electrophoresis and matrix assisted laser desorption ionization - time of flight mass spectrometry-based protein identification of the spots that displayed Western blotting signals with anti-neutrophil cytoplasmic antibody-positive sera. The prevalence of IgG, IgA and secretory IgA [sIgA] to chitinase 3-like protein 1 [CHI3L1] was analysed by enzyme-linked immunosorbent assays using recombinant CHI3L1 in 110 patients with Crohn's disease [CD], 95 with ulcerative colitis [UC], 126 with coeliac disease [CeD] and 86 healthy controls [HCs]. Results The 18-glycosylhydrolase family member CHI3L1 was identified as a neutrophil autoantigenic target. CD patients displayed significantly higher levels of IgG to CHI3L1 than patients with UC and CeD (p < 0.0001, respectively). IgA and sIgA to CHI3L1 was significantly higher in CD than in UC, CeD and HCs [p < 0.0001, respectively]. IgA and sIgA to CHI3L1 demonstrated the highest prevalence in CD [25.5\%, 28/110; and 41.8\%\%, 46/110] compared to HCs [2.3\%, 2/86; and 4.7\%\%, 4/86; p = 0.0015 and p < 0.0001] and are associated with a more complicated progression of CD. Conclusion CHI3L1 is a novel neutrophil autoantigenic target in CD. IgA and sIgA to CHI3L1 may serve as novel markers for CD and may facilitate the serological diagnosis of IBD.}, language = {en} } @misc{SowaMurugaiyanConradetal., author = {Sowa, Mandy and Murugaiyan, Jayaseelan and Conrad, Karsten and Laass, Martin W. and Bogdanos, Dimitrios Petrou and Papp, Maria and R{\"o}diger, Stefan and Roggenbuck, Dirk and Schierack, Peter}, title = {A novel neutrophil autoantigenic target in inflammatory bowel disease}, language = {en} } @misc{LopensKrawczykPappetal., author = {Lopens, Steffi and Krawczyk, Marcin and Papp, Maria and Milkiewicz, Piotr and Schierack, Peter and Liu, Yudong and Wunsch, Ewa and Conrad, Karsten and Roggenbuck, Dirk}, title = {The search for the Holy Grail: autoantigenic targets in primary sclerosing cholangitis asscociated with diesease phenotype an neoplasia}, series = {Autoimmunity Highlights}, volume = {11}, journal = {Autoimmunity Highlights}, issn = {2038-3274}, doi = {10.1186/s13317-020-00129-x}, pages = {14}, language = {en} } @misc{VolkovSeguroLeionetal., author = {Volkov, Ilan and Seguro, Luciana and Leion, Elaine P. and Kov{\´a}cs, L{\´a}szl{\´o} and Roggenbuck, Dirk and Schierack, Peter and Gilburd, Boris and Doria, Andrea and Tektonidou, Maria G. and Agmon-Levin, Nancy}, title = {Profiles of criteria and non-criteria anti-phospholipid autoantibodies are associated with clinical phenotypes of the antiphospholipid syndrome}, series = {Autoimmunity Highlights}, volume = {11}, journal = {Autoimmunity Highlights}, issn = {2038-3274}, doi = {10.1186/s13317-020-00131-3}, pages = {1 -- 8}, language = {en} } @misc{AliKolendaKhanetal., author = {Ali, Aamir and Kolenda, Rafał and Khan, Muhammad Moman and Weinreich, J{\"o}rg and Li, Ganwu and Wieler, Lothar H. and Tedin, Karsten and Roggenbuck, Dirk and Schierack, Peter}, title = {Novel Avian Pathogenic Escherichia coli Genes Responsible for Adhesion to Chicken and Human Cell Lines}, series = {Applied and Environmental Microbiology}, volume = {86}, journal = {Applied and Environmental Microbiology}, number = {20}, issn = {1098-5336}, doi = {10.1128/AEM.01068-20}, language = {en} } @misc{DeutschmannRoggenbuckSchieracketal., author = {Deutschmann, Claudia and Roggenbuck, Dirk and Schierack, Peter and R{\"o}diger, Stefan}, title = {Autoantibody testing by enzyme-linked immunosorbent assay-a case in which the solid phase decides on success and failure}, series = {Heliyon}, volume = {6}, journal = {Heliyon}, number = {1}, issn = {2405-8440}, doi = {10.1016/j.heliyon.2020.e03270}, pages = {6}, language = {en} } @misc{SchiebelNoackRoedigeretal., author = {Schiebel, Juliane and Noack, Jonas and R{\"o}diger, Stefan and Kammel, Anne and Menzel, Friederike and Schwibbert, Karin and Weise, Matthias and Weiss, Romano and B{\"o}hm, Alexander and Nitschke, J{\"o}rg and Elimport, Alexey and Roggenbuck, Dirk and Schierack, Peter}, title = {Analysis of three-dimensional biofilms on different material surfaces}, series = {Biomaterials Science}, volume = {8}, journal = {Biomaterials Science}, number = {12}, issn = {2047-4849}, doi = {10.1039/D0BM00455C}, pages = {3500 -- 3510}, language = {en} } @misc{LiedtkeSchroederRoggenbucketal., author = {Liedtke, Victoria and Schr{\"o}der, Christian and Roggenbuck, Dirk and Weiss, Romano and Stohwasser, Ralf and Schierack, Peter and R{\"o}diger, Stefan and Schenk, Lysann}, title = {LEDGF/p75 is required for an efficient DNA damage response}, series = {International Journal of Molecular Sciences}, volume = {22}, journal = {International Journal of Molecular Sciences}, number = {11}, doi = {10.3390/ijms22115866}, pages = {1 -- 16}, language = {en} } @misc{ComabellaDeutschmannMidagliaetal., author = {Comabella, Manual and Deutschmann, Claudia and Midaglia, Luciana and Schierack, Peter and Mart{\´i}nez, J{\´u}lia and Roggenbuck, Dirk and Montalban, Xavier}, title = {Chitinase 3-like 1 is not a target antigen in patients with multiple sclerosis}, series = {Multiple Sclerosis Journal}, volume = {27}, journal = {Multiple Sclerosis Journal}, number = {9}, issn = {1477-0970}, doi = {10.1177/1352458520980141}, pages = {1455 -- 1457}, language = {en} } @misc{SchmidtBerghausBlessingetal., author = {Schmidt, Jonas and Berghaus, Sandro and Blessing, Frithjof and Wenzel, Folker and Herbeck, Holger and Blessing, Josef and Schierack, Peter and R{\"o}diger, Stefan and Roggenbuck, Dirk}, title = {Serological and viral genetic features of patients with COVID-19 in a selected German patient cohort-correlation with disease characteristics}, series = {GeroScience}, volume = {43}, journal = {GeroScience}, number = {5}, issn = {2509-2723}, doi = {10.1007/s11357-021-00443-w}, pages = {2249 -- 2264}, language = {en} } @misc{SchmidtBorcherdingThieleetal., author = {Schmidt, Carsten and Borcherding, Heike and Thiele, Thomas and Schedler, Uwe and Werner, Franziska and R{\"o}diger, Stefan and Roggenbuck, Dirk and Schierack, Peter}, title = {Fluorescence-encoded poly (methyl metharcylate) nanoparticles for a lateral flow assay detecting IgM autoantibodies in rheumatoid arthritis}, series = {Analytical biochemistry}, volume = {Vol. 633}, journal = {Analytical biochemistry}, issn = {1096-0309}, doi = {10.1016/journal.ppat.1010118}, language = {en} } @misc{EmmeneggerKumarEmmeneggeretal., author = {Emmenegger, Marc and Kumar, Sreedhar Saseendran and Emmenegger, Vishalini and Malinauskas, Tomas and B{\"u}ttner, Thomas and Rose, Laura and Schierack, Peter and Sprinzl, Martin F. and Sommer, Clemens J. and Lackner, Karl J. and Aguzzi, Adriano and Roggenbuck, Dirk and Frauenknecht, Katrin B. M.}, title = {Anti-prothrombin autoantibodies enriched after infection with SARS-CoV-2 and influenced by strength of antibody response against SARS-CoV-2 proteins}, series = {PLoS pathogens}, volume = {17}, journal = {PLoS pathogens}, number = {12}, issn = {1553-7374}, doi = {10.1371/journal.ppat.1010118}, language = {en} } @misc{SchmidtBerghausBlessingetal., author = {Schmidt, Jonas and Berghaus, Sandro and Blessing, Frithjof and Wenzel, Folker and Herbeck, Holger and Blessing, Josef and Schierack, Peter and R{\"o}diger, Stefan and Roggenbuck, Dirk}, title = {A semi-automated, isolation-free, high-throughput SARS-CoV-2 reverse transcriptase (RT) loop-mediated isothermal amplification (LAMP) test}, series = {Scientific reports}, volume = {11}, journal = {Scientific reports}, number = {1}, issn = {2045-2322}, doi = {10.1038/s41598-021-00827-0}, language = {en} } @misc{BartlitzKolendaChilimoniuketal., author = {Bartlitz, Christin and Kolenda, Rafał and Chilimoniuk, Jarosław and Grzymajlo, Krzysztof and R{\"o}diger, Stefan and Bauerfeind, Rolf and Ali, Aamir and Tchesnokovag, Veronika and Roggenbuck, Dirk and Schierack, Peter}, title = {Adhesion of Enteropathogenic, Enterotoxigenic, and Commensal Escherichia coli to the Major Zymogen Granule Membrane Glyoprotein 2}, series = {Applied abd Environmental Microbiology}, volume = {88}, journal = {Applied abd Environmental Microbiology}, number = {5}, issn = {1098-5536}, doi = {10.1128/aem.02279-21}, language = {en} } @misc{LopensWunschMilkiewiczetal., author = {Lopens, Steffi and Wunsch, Ewa and Milkiewicz, Malgorzata and R{\"o}ber, Nadja and Zarske, Grit and Nasser, Abdullah and Conrad, Karsten and Laass, Martin W. and R{\"o}diger, Stefan and Krawczyk, Marcin and Roggenbuck, Dirk and Milkiewicz, Piotr}, title = {PR3-ANCAs Detected by Third-Generation ELISA Predicts Severe Disease and Poor Survival in Primary Sclerosing Cholangitis}, series = {Diagnostics}, volume = {12}, journal = {Diagnostics}, number = {11}, issn = {2075-4418}, doi = {10.3390/diagnostics12112682}, abstract = {A highly sensitive detection of anti-neutrophil cytoplasmic antibodies to serine proteinase-3 (PR3-ANCAs) aids in the serological diagnosis of autoimmune liver disorders and the prediction of severity in primary sclerosing cholangitis (PSC). Here, we evaluate a novel third-generation ELISA for the detection of PR3-ANCAs. In total, 309 patients with PSC, 51 with primary biliary cholangitis (PBC), and 120 healthy blood donors (BD) were analyzed. For the survival analysis in PSC, the outcome was defined as liver-transplantation-free survival during the follow-up. Positive PR3-ANCA levels were found in 74/309 (24.0\%) of patients with PSC. No BDs and one patient with PBC demonstrated PR3-ANCA positivity. PR3-ANCAs were revealed as independent predictors for a poor PSC outcome (study endpoint: liver transplantation/death, log-rank test, p = 0.02). PR3-ANCA positivity, lower albumin levels, and higher bilirubin concentrations were independent risks of a poor survival (Cox proportional-hazards regression analysis, p < 0.05). The Mayo risk score for PSC was associated with PR3-ANCA positivity (p = 0.01) and the disease severity assessed with a model of end-stage liver disease (MELD) and extended MELD-Na (p < 0.05). PR3-ANCAs detected by a third-generation ELISA are diagnostic and prognostic markers for PSC. Their wider use could help to identify patients who are at-risk of a more severe disease.}, language = {en} } @misc{HiemannRoggenbuckSacketal., author = {Hiemann, Rico and Roggenbuck, Dirk and Sack, Ulrich and Anderer, Ursula and Conrad, Karsten}, title = {Die HEp-2-Zelle als Target f{\"u}r multiparametrische Autoantik{\"o}rperanalytik - Automatisierung und Standardisierung/The HEp-2 cell as target for multiparametric autoantibody analyses: automation and standardisation}, series = {Journal of Laboratory Medicine}, volume = {35}, journal = {Journal of Laboratory Medicine}, number = {6}, issn = {1439-0477}, doi = {10.1515/JLM.2011.053}, pages = {351 -- 361}, abstract = {Trotz Einf{\"u}hrung von Multiplex-Assays ist die Bestimmung von Autoantik{\"o}rpern (AAK) mittels indirekter Immunfluoreszenz (IIF) nach wie vor ein wesentlicher Bestandteil der Autoimmundiagnostik. Das Screening auf nichtorganspezifische AAK wird in der Routinediagnostik von autoimmunen rheumatischen Erkrankungen mittels indirekter Immunfluoreszenz an HEp-2-Zellen durchgef{\"u}hrt. Leider differieren die mit diesem Test erhaltenen Befunde z. T. recht erheblich auf Grund von objektiven (z. B. HEp-2-Zellfixierung) und subjektiven (z. B. Erfahrungswerte) Faktoren. Die Intra- und Interlaborvarianzen sind daher relativ hoch. Erste Ergebnisse von Evaluierungsstudien eines neu entwickelten Systems zur automatischen Bildanalyse (AKLIDES®) zeigen einen Weg in Richtung Standardisierung (bzw. Harmonisierung) des HEp-2-Zell-Assays auf, in dem zun{\"a}chst subjektive Fehlinterpretationen vermieden werden k{\"o}nnen. Eine weitere Harmonisierung erfordert die Weiterentwicklung der Software-Algorithmen zur Mustererkennung sowie neue Kalibrationssysteme.}, language = {en} } @misc{AzamRoesslingGeitheetal., author = {Azam, Hafiz Muhammad Husnain and R{\"o}ßling, Rosa Ilse and Geithe, Christiane and Khan, Muhammad Moman and Dinter, Franziska and Hanack, Katja and Pr{\"u}ß, Harald and Husse, Britta and Roggenbuck, Dirk and Schierack, Peter and R{\"o}diger, Stefan}, title = {MicroRNA biomarkers as next-generation diagnostic tools for neurodegenerative diseases: a comprehensive review}, series = {Frontiers in Molecular Neuroscience}, volume = {17}, journal = {Frontiers in Molecular Neuroscience}, publisher = {Frontiers Media S.A.}, issn = {1662-5099}, doi = {10.3389/fnmol.2024.1386735}, pages = {35}, abstract = {Neurodegenerative diseases (NDs) are characterized by abnormalities within neurons of the brain or spinal cord that gradually lose function, eventually leading to cell death. Upon examination of affected tissue, pathological changes reveal a loss of synapses, misfolded proteins, and activation of immune cells—all indicative of disease progression—before severe clinical symptoms become apparent. Early detection of NDs is crucial for potentially administering targeted medications that may delay disease advancement. Given their complex pathophysiological features and diverse clinical symptoms, there is a pressing need for sensitive and effective diagnostic methods for NDs. Biomarkers such as microRNAs (miRNAs) have been identified as potential tools for detecting these diseases. We explore the pivotal role of miRNAs in the context of NDs, focusing on Alzheimer's disease, Parkinson's disease, Multiple sclerosis, Huntington's disease, and Amyotrophic Lateral Sclerosis. The review delves into the intricate relationship between aging and NDs, highlighting structural and functional alterations in the aging brain and their implications for disease development. It elucidates how miRNAs and RNA-binding proteins are implicated in the pathogenesis of NDs and underscores the importance of investigating their expression and function in aging. Significantly, miRNAs exert substantial influence on post-translational modifications (PTMs), impacting not just the nervous system but a wide array of tissues and cell types as well. Specific miRNAs have been found to target proteins involved in ubiquitination or de-ubiquitination processes, which play a significant role in regulating protein function and stability. We discuss the link between miRNA, PTM, and NDs. Additionally, the review discusses the significance of miRNAs as biomarkers for early disease detection, offering insights into diagnostic strategies.}, language = {en} } @misc{FrostWeissHerteletal., author = {Frost, Fabian and Weiss, Stefan and Hertel, Johannes and R{\"u}hlemann, Malte and Bang, Corinna and Franke, Andre and Nauck, Matthias and D{\"o}rr, Marcus and V{\"o}lzke, Henry and Roggenbuck, Dirk and Schierack, Peter and V{\"o}lker, Uwe and Homuth, Georg and Aghdassi, Ali A. and Sendler, Matthias and Lerch, Markus M. and Weiss, Frank U.}, title = {Fecal glycoprotein 2 is a marker of gut microbiota dysbiosis and systemic inflammation}, series = {Gut Pathogens}, volume = {16}, journal = {Gut Pathogens}, number = {1}, publisher = {Springer Science and Business Media LLC}, issn = {1757-4749}, doi = {10.1186/s13099-024-00657-1}, pages = {1 -- 11}, abstract = {Background autoantigenic glycoprotein 2 (GP2) is an important component of the innate immune system which originates from the exocrine pancreas as well as from the small intestines. The relationship of GP2 with the intestinal microbiome as well as the systemic implications of increased fecal GP2 levels are, however, still unclear. Therefore, fecal samples from 2,812 individuals of the Study of Health in Pomerania (SHIP) were collected to determine GP2 levels (enzyme-linked immunosorbent assay) and gut microbiota profiles (16 S rRNA gene sequencing). These data were correlated and associated with highly standardised and comprehensive phenotypic data of the study participants. Results Fecal GP2 levels were increased in individuals with higher body mass index and smokers, whereas lower levels were found in case of preserved exocrine pancreatic function, female sex or a healthier diet. Moreover, higher GP2 levels were associated with increased serum levels of high-sensitivity C-reactive protein, loss of gut microbial diversity and an increase of potentially detrimental bacteria (Streptococcus, Haemophilus, Clostridium XIVa, or Collinsella). At the same time, predicted microbial pathways for the biosynthesis of beneficial short-chain fatty acids or lactic acid were depleted in individuals with high fecal GP2. Of note, GP2 exhibited a stronger association to overall microbiome variation than calprotectin. Conclusion Fecal GP2 is a biomarker of gut microbiota dysbiosis and associated with increased systemic inflammation. The intestines may be more important as origin for GP2 than pancreatic acinar cells. Future studies need to investigate the potential clinical value in disease specific patient cohorts.}, language = {en} } @misc{LopensSchierackKrauseetal., author = {Lopens, Steffi and Schierack, Peter and Krause, Jenny and Piaszczyński, Michał and Kr{\´o}l, Robert and Staroń, Robert and Krupa, Łukasz and Gutkowski, Krzysztof and Kruk, Beata and Grąt, Michał and Krawczyk, Marek and Patkowski, Waldemar and Glaser, Fabian and R{\"o}diger, Stefan and Grossmann, Kai and Pająk, Jacek and Milkiewicz, Piotr and Lammert, Frank and Zieniewicz, Krzysztof and Schramm, Christoph and Roggenbuck, Dirk and Krawczyk, Marcin}, title = {Antimicrobial glycoprotein 2 (GP2) in gallstones, bile fluid and peribiliary glands of patients with primary sclerosing cholangitis}, series = {Clinica Chimica Acta}, volume = {562}, journal = {Clinica Chimica Acta}, publisher = {Elsevier BV}, issn = {0009-8981}, doi = {10.1016/j.cca.2024.119841}, pages = {1 -- 8}, language = {en} } @misc{GeitheZengSchmidtetal., author = {Geithe, Christiane and Zeng, Bo and Schmidt, Carsten and Dinter, Franziska and Roggenbuck, Dirk and Lehmann, Werner and Dame, Gregory and Schierack, Peter and Hanack, Katja and R{\"o}diger, Stefan}, title = {A multiplex microchamber diffusion assay for the antibody-based detection of microRNAs on randomly ordered microbeads}, series = {Biosensors and Bioelectronics: X}, volume = {18 (2024)}, journal = {Biosensors and Bioelectronics: X}, publisher = {Elsevier BV}, issn = {2590-1370}, doi = {10.1016/j.biosx.2024.100484}, pages = {1 -- 7}, language = {en} } @misc{LiedtkeStoeckleJunkeretal., author = {Liedtke, Victoria and St{\"o}ckle, Michael and Junker, Kerstin and Roggenbuck, Dirk}, title = {Benign prostatic hyperplasia - a novel autoimmune disease with a potential therapy consequence?}, series = {Autoimmunity Reviews}, volume = {23}, journal = {Autoimmunity Reviews}, number = {3}, publisher = {Elsevier BV}, issn = {1568-9972}, doi = {10.1016/j.autrev.2023.103511}, pages = {6}, abstract = {Benign prostatic hyperplasia (BPH) is considered as an age-related disease of men with an unknown etiopathophysiology. Chronic inflammation has been proposed as one of the major pathophysiological mechanisms. There is growing evidence for the involvement of autoimmune responses in an inflammatory setting in the prostate. Patients with autoimmune diseases show a significantly elevated prevalence of BPH. Conventional therapy options for BPH are limited, rendering surgery the ultimate alternative. However, immunosuppression via tumor necrosis factor alpha blocker appears to reduce symptoms in patients with BPH and concurrent autoimmune disease due to the reduction of epithelial hyperplasia and macrophage-induced inflammation. New diagnostic options using HEp-2 cells with overexpression of LEDGF/p75 or mitochondrial DNA as autoimmune targets could be used to identify BPH patients with autoimmune responses. Given the presumed involvement of autoimmune responses in BPH and the efficacy of immunosuppression in reducing BPH symptoms, BPH or subvariants of BPH may be candidates for a new autoimmune disease in males.}, language = {en} } @misc{LiedtkeRoseHiemannetal., author = {Liedtke, Victoria and Rose, Laura and Hiemann, Rico and Nasser, Abdullah and R{\"o}diger, Stefan and Bonaventura, Alena and Winkler, Laura and Sowa, Mandy and St{\"o}ckle, Michael and Schierack, Peter and Junker, Kerstin and Roggenbuck, Dirk}, title = {Over-Expression of LEDGF/p75 in HEp-2 Cells Enhances Autoimmune IgG Response in Patients with Benign Prostatic Hyperplasia—A Novel Diagnostic Approach with Therapeutic Consequence?}, series = {International Journal of Molecular Sciences}, volume = {24}, journal = {International Journal of Molecular Sciences}, number = {7}, issn = {1422-0067}, doi = {10.3390/ijms24076166}, abstract = {Lens epithelium-derived growth factor splice variant of 75 kDa (LEDGF/p75) is an autoantigen over-expressed in solid tumors and acts as a stress-related transcriptional co-activator. Participation of autoimmune responses in the pathophysiology of benign prostatic hyperplasia (PBH) and a corresponding immunosuppressive therapy by TNFalpha antagonists has been recently suggested. Thus, autoAb testing could aid in the diagnosis of BPH patients profiting from such therapy. We generated CRISPR/Cas9 modified HEp-2 LEDGF knock-out (KO) and HEp-2 LEDGF/p75 over-expressing (OE) cells and examined IgG autoantibody reactivity to LEDGF/p75 in patients with prostate cancer (PCa, n = 89), bladder cancer (BCa, n = 116), benign prostatic hyperplasia (BPH, n = 103), and blood donors (BD, n = 60) by indirect immunofluorescence assay (IFA). Surprisingly, we could not detect elevated binding of autoAbs against LEDGF/p75 in cancer patients, but autoAb reactivity to LEDGF/p75 OE cells in about 50\% of patients with BPH was unexpectedly significantly increased. Furthermore, a line immunoassay enabling the detection of 18 different autoAbs revealed a significantly increased occurrence of anti-dsDNA autoAbs in 34\% of BPH patients in contrast to tumor patients and BD. This finding was confirmed by anti-mitochondrial (mDNA) autoAb detection with the Crithidia luciliae immunofluorescence test, which also showed a significantly higher prevalence (34\%) of anti-mDNA autoAbs in BPH. In summary, our study provided further evidence for the occurrence of autoimmune responses in BPH. Furthermore, LEDGF/p75 over-expression renders HEp-2 cells more autoantigenic and an ideal target for autoAb analysis in BPH with a potential therapy consequence.}, language = {en} } @misc{RoeiBorisYehudaetal., author = {Roei, Tulchinsky and Boris, Gilburd and Yehuda, Shovman and Milena, Tocut and Eleanor, Zeruya and Ariel, Binyaminov and Tima, Davidson and B{\"u}ttner, Thomas and Nasser, Abdullah and Michel, Juliane and R{\"o}diger, Stefan and Schierack, Peter and Howard, Amital and Roggenbuck, Dirk and Yehuda, Shoenfeld and Ora, Shovman}, title = {CytoBead ANA 2 assay : a novel method for the detection of antinuclear antibodies}, series = {Scientific reports}, volume = {15}, journal = {Scientific reports}, number = {1}, publisher = {Nature Publishing Group UK}, address = {London}, issn = {2045-2322}, doi = {10.1038/s41598-025-04583-3}, pages = {1 -- 11}, abstract = {Detection of anti-nuclear autoantibodies (ANA) is based on a two-step algorithm including indirect immunofluorescence (IIF) on HEp2 cells and subsequent reflex/confirmatory testing for specific autoantibodies. Simultaneous cell- and microbead-based autoantibody detection by IIF may be utilized for the evaluation of systemic autoimmune rheumatic diseases (SARDs). In the present study, we assessed the performance of CytoBead ANA 2 in the detection of ANA and ANA-specific autoantibodies, compared to ANA IIF and BioPlex™ 2200. We also tested the ability of CytoBead ANA DFS-70 to identify dense-fine speckled (DFS) pattern associated with anti-DFS70 antibodies in non-SARDs patients. Hundred-twelve routine sera samples were assessed by manual CytoBead ANA 2 for the presence of ANA and specific autoantibodies. In parallel, these samples were analyzed by HEp2 ANA IIF test and a subsequent multiplexed assay BioPlex™ 2200 ANA. Twenty-nine ANA-positive samples obtained from non-SARDs patients and exhibiting DFS pattern by ANA IIF were further tested by CytoBead ANA DFS-70. A substantial agreement was observed between classical ANA IIF and manual CytoBead ANA 2 for the detection of ANA (k = 0.74). Discordant results were mainly associated with the presence of anti-SSA/Ro antibodies detected by CytoBead ANA 2 in ANA IIF negative patients. A good to almost perfect agreement was found between CytoBead ANA 2 and BioPlex™ 2200 for detection of specific antibodies with kappa values ranging from 0.70 to 0.90. Twenty samples (68.9\%) obtained from 29 ANA IIF positive without SARDs patients exhibited DFS pattern in CytoBead ANA DFS-70, which confirmed the presence of anti-DFS70 antibodies. The diagnostic performance of manual CytoBead ANA 2 for ANA screening and detection of ANA specific antibodies is comparable to the diagnostic performance of ANA IIF followed by BioPlex™ 2200. This novel one-step assay enables simultaneous ANA screening and confirmation and represents a promising alternative approach to the time-consuming and costly two-tier ANA analysis.}, language = {en} }