@misc{SchierackRoedigerKuhletal., author = {Schierack, Peter and R{\"o}diger, Stefan and Kuhl, Christoph and Hiemann, Rico and Roggenbuck, Dirk and Li, Ganwu and Weinreich, J{\"o}rg and Berger, Enrico and Nolan, Lisa K. and Nicholson, Bryon and R{\"o}mer, Antje and Fr{\"o}mmel, Ulrike and Wieler, Lothar H. and Schr{\"o}der, Christian}, title = {Porcine E. coli: Virulence-Associated Genes, Resistance Genes and Adhesion and Probiotic Activity Tested by a New Screening Method}, series = {PLoS One}, volume = {8}, journal = {PLoS One}, number = {4}, doi = {10.1371/journal.pone.0059242}, pages = {13}, abstract = {We established an automated screening method to characterize adhesion of Escherichia coli to intestinal porcine epithelial cells (IPEC-J2) and their probiotic activity against infection by enteropathogenic E. coli (EPEC). 104 intestinal E. coli isolates from domestic pigs were tested by PCR for the occurrence of virulence-associated genes, genes coding for resistances to antimicrobial agents and metals, and for phylogenetic origin by PCR. Adhesion rates and probiotic activity were examined for correlation with the presence of these genes. Finally, data were compared with those from 93 E. coli isolates from wild boars. Isolates from domestic pigs carried a broad variety of all tested genes and showed great diversity in gene patterns. Adhesions varied with a maximum of 18.3 or 24.2 mean bacteria adherence per epithelial cell after 2 or 6 hours respectively. Most isolates from domestic pigs and wild boars showed low adherence, with no correlation between adhesion/probiotic activity and E. coli genes or gene clusters. The gene sfa/foc, encoding for a subunit of F1C fimbriae did show a positive correlative association with adherence and probiotic activity; however E. coli isolates from wild boars with the sfa/foc gene showed less adhesion and probiotic activity than E. coli with the sfa/foc gene isolated from domestic pigs after 6 hour incubation. In conclusion, screening porcine E. coli for virulence associated genes genes, adhesion to intestinal epithelial cells, and probiotic activity revealed a single important adhesion factor, several probiotic candidates, and showed important differences between E. coli of domestic pigs and wild boars.}, language = {en} } @misc{FroemmelLehmannRoedigeretal., author = {Fr{\"o}mmel, Ulrike and Lehmann, Werner and R{\"o}diger, Stefan and B{\"o}hm, Alexander and Nitschke, J{\"o}rg and Weinreich, J{\"o}rg and Groß, Julia and Roggenbuck, Dirk and Zinke, Olaf and Ansorge, Hermann and Vogel, Steffen and Klemm, Per and Wex, Thomas and Schr{\"o}der, Christian and Wieler, Lothar H. and Schierack, Peter}, title = {Adhesion of human and animal Escherichia coli strains in association with their virulence-associated genes and phylogenetic origins}, series = {Applied and environmental microbiology}, volume = {79}, journal = {Applied and environmental microbiology}, number = {19}, issn = {1098-5336}, doi = {10.1128/AEM.01384-13}, pages = {5814 -- 5829}, abstract = {Intestinal colonization is influenced by the ability of the bacterium to inhabit a niche, which is based on the expression of colonization factors. Escherichia coli carries a broad range of virulence-associated genes (VAGs) which contribute to intestinal (inVAGs) and extraintestinal (exVAGs) infection. Moreover, initial evidence indicates that inVAGs and exVAGs support intestinal colonization. We developed new screening tools to genotypically and phenotypically characterize E. coli isolates originating in humans, domestic pigs, and 17 wild mammal and avian species. We analyzed 317 isolates for the occurrence of 44 VAGs using a novel multiplex PCR microbead assay (MPMA) and for adhesion to four epithelial cell lines using a new adhesion assay. We correlated data for the definition of new adhesion genes. inVAGs were identified only sporadically, particularly in roe deer (Capreolus capreolus) and the European hedgehog ( Erinaceus europaeus). The prevalence of exVAGs depended on isolation from a specific host. Human uropathogenic E. coli isolates carried exVAGs with the highest prevalence, followed by badger (Meles meles) and roe deer isolates. Adhesion was found to be very diverse. Adhesion was specific to cells, host, and tissue, though it was also unspecific. Occurrence of the following VAGs was associated with a higher rate of adhesion to one or more cell lines: afa-dra, daaD, tsh, vat, ibeA, fyuA, mat, sfa-foc, malX, pic, irp2, and papC. In summary, we established new screening methods which enabled us to characterize large numbers of E. coli isolates. We defined reservoirs for potential pathogenic E. coli. We also identified a very broad range of colonization strategies and defined potential new adhesion genes.}, language = {en} } @misc{FroemmelBoehmNitschkeetal., author = {Fr{\"o}mmel, Ulrike and B{\"o}hm, Alexander and Nitschke, J{\"o}rg and Weinreich, J{\"o}rg and Groß, Julia and R{\"o}diger, Stefan and Wex, Thomas and Ansorge, Hermann and Zinke, Olaf and Schr{\"o}der, Christian and Roggenbuck, Dirk and Schierack, Peter}, title = {Adhesion patterns of commensal and pathogenic Escherichia coli from humans and wild animals on human and porcine epithelial cell lines}, series = {Gut Pathogens}, volume = {5}, journal = {Gut Pathogens}, number = {31}, issn = {1757-4749}, doi = {10.1186/1757-4749-5-31}, pages = {8}, abstract = {Abstract BACKGROUND: Different strategies of colonization or infection by E. coli result in formation of certain adhesion patterns which help also in classifying intestinal E. coli into pathotypes. Little is known about adhesion patterns and host- and tissue adaption of commensal E. coli and about E. coli originating in clinically healthy hosts carrying pathotype-specific virulence-associated genes. FINDINGS: Adhesion pattern of E. coli (n = 282) from humans and from 18 animal species were verified on intestinal human Caco-2 and porcine IPEC-J2 cells and, furthermore, for comparison on human urinary bladder 5637, porcine kidney PK-15 epithelial and HEp-2 cells. The analysis was carried out on 150,000 images of adhesion assays.Adhesion patterns were very diverse; 88 isolates were completely non-adherent, whereas 194 adhered to at least one cell line with the dominant adhesion patterns "diffusely distributed" and "microcolony formation". Adhesion patterns "chains" and "clumps" were also visible. Chain formation was mediated by the presence of epithelial cells. Clump formation was very specific on only the 5637 cell line. All enteropathogenic (eae+) E. coli (EPEC; n = 14) were able to form microcolonies which was cell line specific for each isolate. Most EPEC formed microcolonies on intestinal IPEC-J2 and Caco-2 but several also on urinary tract cells. Shigatoxin-producing (stx+) E. coli (n = 10) showed no specific adhesion patterns. CONCLUSIONS: E. coli isolates were highly diverse. Commensal and pathogenic isolates can adhere in various forms, including diffuse distribution, microcolonies, chains and clumps. Microcolony formation seems to be a global adhesion strategy also for commensal E. coli.}, language = {en} } @misc{GrossmannRoggenbuckSchroederetal., author = {Großmann, Kai and Roggenbuck, Dirk and Schr{\"o}der, Christian and Conrad, Karsten and Schierack, Peter and Sack, Ulrich}, title = {Multiplex assessment of non-organ-specific autoantibodies with a novel microbead-based immunoassay}, series = {Cytometry Part A}, volume = {79}, journal = {Cytometry Part A}, number = {2}, issn = {1552-4922}, doi = {10.1002/cyto.a.21009}, pages = {118 -- 125}, language = {en} } @incollection{RoedigerSchierackBoehmetal., author = {R{\"o}diger, Stefan and Schierack, Peter and B{\"o}hm, Alexander and Nitschke, J{\"o}rg and Berger, Ingo and Fr{\"o}mmel, Ulrike and Schmidt, Carsten and Ruhland, Mirko and Schimke, Ingolf and Roggenbuck, Dirk and Lehmann, Werner and Schr{\"o}der, Christian}, title = {A highly versatile microscope imaging technology platform for the multiplex real-time detection of biomolecules and autoimmune antibodies}, series = {Molecular diagnostics}, booktitle = {Molecular diagnostics}, editor = {Seitz, Harald and Berger, Ingo}, publisher = {Springer}, address = {Berlin [u.a.]}, isbn = {978-3-642-37690-0}, pages = {35 -- 74}, language = {en} } @misc{NalliSommaAndreolietal., author = {Nalli, Cecilia and Somma, Valentina and Andreoli, Laura and B{\"u}ttner, Thomas and Schierack, Peter and Mahler, Michael and Roggenbuck, Dirk and Tincani, Angela}, title = {Anti-phospholipid IgG antibodies detected by line immunoassay differentiate patients with anti-phospholipid syndrome and other autoimmune diseases}, series = {Autoimmunity Highlights}, volume = {9}, journal = {Autoimmunity Highlights}, number = {1}, issn = {2038-3274}, doi = {10.1007/s13317-018-0106-0}, pages = {11}, language = {en} } @misc{KolendaBurdukiewiczSchiebeletal., author = {Kolenda, Rafał and Burdukiewicz, Michał and Schiebel, Juliane and R{\"o}diger, Stefan and Sauer, Lysann and Szabo, Istvan and Orlowska, Aleksandra and Weinreich, J{\"o}rg and Nitschke, J{\"o}rg and B{\"o}hm, Alexander and Gerber, Ulrike and Roggenbuck, Dirk and Schierack, Peter}, title = {Adhesion of Salmonella to Pancreatic Secretory Granule Membrane Major Glycoprotein GP2 of Human and Porcine Origin Depends on FimH Sequence Variation}, series = {Frontiers in Microbiology}, volume = {9}, journal = {Frontiers in Microbiology}, number = {1905}, issn = {1664-302X}, doi = {10.3389/fmicb.2018.01905}, pages = {15}, language = {en} } @misc{SowaKolendaBaumgartetal., author = {Sowa, Mandy and Kolenda, Rafał and Baumgart, Daniel C. and Pratschke, Johann and Papp, Maria and Tornai, Tamas and Suchanski, Jaroslaw and Bogdanos, Dimitrios Petrou and Mytilinaiou, Maria G. and Hammermann, Jutta and Laass, Martin W. and Conrad, Karsten and Schramm, Christoph and Franke, Andre and Roggenbuck, Dirk and Schierack, Peter}, title = {Mucosal Autoimmunity to Cell-Bound GP2 Isoforms Is a Sensitive Marker in PSC and Associated With the Clinical Phenotype}, series = {Frontiers in Immunology}, volume = {9}, journal = {Frontiers in Immunology}, number = {1959}, issn = {1664-3224}, doi = {10.3389/fimmu.2018.01959}, language = {en} } @misc{MaslyanskiyLazarevaOlineketal., author = {Maslyanskiy, Aleksey and Lazareva, Natalya and Olinek, Polina and Schierack, Peter and Hentschel, Christian and Cuccato, Juliane and Bogdanos, Dimitrios Petrou and Lapin, Sergey V. and Roggenbuck, Dirk}, title = {Anti-hnRNP B1 (RA33) Autoantibodies Are Associated with the Clinical Phenotype in Russian Patients with Rheumatoid Arthritis and Systemic Sclerosis}, series = {Journal of Immunology Research}, volume = {2014}, journal = {Journal of Immunology Research}, doi = {10.1155/2014/516593}, pages = {7}, language = {en} } @misc{MirskyRoedigerSchieracketal., author = {Mirsky, Vladimir M. and R{\"o}diger, Stefan and Schierack, Peter and Roggenbuck, Dirk}, title = {Preface to the Special Issue on PCR on chip and related technologies}, series = {Microchimica Acta}, volume = {181}, journal = {Microchimica Acta}, number = {13/14}, doi = {10.1007/s00604-014-1246-1}, pages = {1609 -- 1610}, language = {en} } @misc{SowaGrossmannScholzetal., author = {Sowa, Mandy and Großmann, Kai and Scholz, Juliane and R{\"o}ber, Nadja and R{\"o}diger, Stefan and Schierack, Peter and Conrad, Karsten and Roggenbuck, Dirk and Hiemann, Rico}, title = {Der CytoBead-Assay - Eine neue M{\"o}glichkeit der multiparametrischen Autoantik{\"o}rperanalytik bei systemischen Autoimmunerkrankungen}, series = {Journal of Laboratory Medicine}, volume = {38}, journal = {Journal of Laboratory Medicine}, number = {6}, doi = {10.1515/labmed-2014-0041}, pages = {309 -- 317}, language = {de} } @misc{RoggenbuckSommaSchieracketal., author = {Roggenbuck, Dirk and Somma, Valentina and Schierack, Peter and Borghi, Maria Orietta and Meroni, Pier Luigi}, title = {Autoantibody profiling in APS}, series = {Lupus}, volume = {23}, journal = {Lupus}, number = {12}, issn = {0961-2033}, doi = {10.1177/0961203314534305}, pages = {1262 -- 1264}, language = {en} } @misc{RoggenbuckBorghiSommaetal., author = {Roggenbuck, Dirk and Borghi, Maria Orietta and Somma, Valentina and B{\"u}ttner, Thomas and Schierack, Peter and Hanack, Katja and Grossi, Claudia and Bodio, Caterina and Macor, Paolo and Landenberg, Philipp von and Boccellato, Francesco and Mahler, Michael and Meroni, Pier Luigi}, title = {Antiphospholipid antibodies detected by line immunoassay differentiate among patients with antiphospholipid syndrome, with infections and asymptomatic carriers}, series = {Arthritis Research \& Therapy}, volume = {18}, journal = {Arthritis Research \& Therapy}, number = {111}, issn = {1465-9913}, doi = {10.1186/s13075-016-1018-x}, pages = {14}, language = {en} } @misc{SowaTrezziHiemannetal., author = {Sowa, Mandy and Trezzi, Barbara and Hiemann, Rico and Schierack, Peter and Großmann, Kai and Scholz, Juliane and Somma, Valentina and Sinico, Renato Alberto and Roggenbuck, Dirk and Radice, Antonella}, title = {Simultaneous comprehensive multiplex autoantibody analysis for rapidly progressive glomerulonephritis}, series = {Medicine}, volume = {95}, journal = {Medicine}, number = {44}, issn = {0025-7974}, doi = {10.1097/MD.0000000000005225}, pages = {5225}, language = {en} } @misc{RoedigerKramerFroemmeletal., author = {R{\"o}diger, Stefan and Kramer, Toni and Fr{\"o}mmel, Ulrike and Weinreich, J{\"o}rg and Roggenbuck, Dirk and Guenther, Sebastian and Schaufler, Katharina and Schr{\"o}der, Christian and Schierack, Peter}, title = {Intestinal Escherichia coli colonization in a mallard duck population over four consecutive winter season}, series = {Environmental Microbiology}, volume = {17}, journal = {Environmental Microbiology}, number = {9}, issn = {1462-2920}, doi = {10.1111/1462-2920.12807}, pages = {3352 -- 3361}, language = {en} } @misc{VillaltaMytilinaiouElsneretal., author = {Villalta, Danilo and Mytilinaiou, Maria G. and Elsner, Martina and Hentschel, Christian and Cuccato, Juliane and Somma, Valentina and Schierack, Peter and Roggenbuck, Dirk and Bogdanos, Dimitrios Petrou}, title = {Autoantibodies to asialoglycoprotein receptor (ASGPR) in patients with autoimmune liver diseases}, series = {Clinica Chimica Acta}, volume = {450}, journal = {Clinica Chimica Acta}, issn = {0009-8981}, doi = {10.1016/j.cca.2015.07.021}, pages = {5}, language = {en} } @inproceedings{RoedigerBoehmNitschkeetal., author = {R{\"o}diger, Stefan and B{\"o}hm, Alexander and Nitschke, J{\"o}rg and Stavitskaya, Luba and Gruner, Melanie and Kundzer, Alena V. and Volkova, Margarita V. and Generalov, I. and Schmidt, Carsten and Schr{\"o}der, Christian and Roggenbuck, Dirk and Schierack, Peter}, title = {Development of a Method for Multiplex Real-Time Analysis of Enzymatic Activity on a Microbead-Chip}, series = {Infection, tumors and autoimmunity, report on the 11th Dresden Symposium on Autoantibodies held in Dresden on September 1-4, 2013}, booktitle = {Infection, tumors and autoimmunity, report on the 11th Dresden Symposium on Autoantibodies held in Dresden on September 1-4, 2013}, publisher = {Pabst Science Publ.}, address = {Lengerich [u.a.]}, isbn = {978-3-89967-881-9}, pages = {275 -- 280}, language = {en} } @inproceedings{RoedigerBurdukiewiczHeiserichetal., author = {R{\"o}diger, Stefan and Burdukiewicz, Michał and Heiserich, Lisa and Schierack, Peter and Roggenbuck, Dirk}, title = {Digital Enumeration of Double Strand Breaks via γH2AX and Aassociated biomarkers in a Computing Environment for Reproducible Research}, series = {From autoantibody research to standardized diagnostic assays in the management of human diseases, report on the 12th Dresden Symposium on Autoantibodies, September 23-26, 2015}, booktitle = {From autoantibody research to standardized diagnostic assays in the management of human diseases, report on the 12th Dresden Symposium on Autoantibodies, September 23-26, 2015}, publisher = {Pabst Science Publishers}, address = {Lengerich}, isbn = {978-3-95853-104-8}, pages = {S. 413}, language = {en} } @misc{RoedigerSchneiderSaueretal., author = {R{\"o}diger, Stefan and Schneider, Jens and Sauer, Lysann and Reddig, Annika and Schierack, Peter and Roggenbuck, Dirk and Schr{\"o}der, Christian}, title = {A Multiparameter Platform for The Analysis of DNA Double-strand Breaks and Associated Biomarkers after genome Editing Austrian Biomarker Symposium (ASBD) 2016, 10.-11.03.2016, Wien, {\"O}sterreich}, language = {en} } @misc{SauerRoedigerSchneideretal., author = {Sauer, Lysann and R{\"o}diger, Stefan and Schneider, Jens and Schierack, Peter and Roggenbuck, Dirk and Schr{\"o}der, Christian}, title = {Functional Multiparameter Analy sis of Double-Strand Breaks and Associate Biomarkers during Genome Editing, Genome Editing and Gene Modulation Congress 2016, 6.-8.04.2016, Oxford, United Kingdom}, language = {en} } @misc{DeutschmannDinterRoedigeretal., author = {Deutschmann, Claudia and Dinter, Franziska and R{\"o}diger, Stefan and Roggenbuck, Dirk and Schierack, Peter}, title = {Comparison of Lab and Point of Care (POC) technologies - case study for CHI3L1, Potsdam Days on Bioanalysis}, pages = {1}, language = {en} } @inproceedings{RoedigerBoehmNitschkeetal., author = {R{\"o}diger, Stefan and B{\"o}hm, Alexander and Nitschke, J{\"o}rg and Stavitskaya, Luba and Gruner, Melanie and Kundzer, Alena V. and Volkova, Margarita V. and Generalov, I. and Schmidt, Carsten and Schr{\"o}der, Christian and Roggenbuck, Dirk and Schierack, Peter}, title = {Development of a Method for Multiplex Real-Time Analysis of Enzymati c Activity on a Microbead-Chip.}, series = {Infections, Tumors and Autoimmunity, Report on the 11th Dresden Symposium on Autoantibodies held in Dresden on September 01. - 04., 2013}, booktitle = {Infections, Tumors and Autoimmunity, Report on the 11th Dresden Symposium on Autoantibodies held in Dresden on September 01. - 04., 2013}, publisher = {Pabst Science Publ.}, address = {Lengerich}, isbn = {978-3-89967-881-9}, pages = {280}, language = {en} } @misc{SchiebelBoehmNitschkeetal., author = {Schiebel, Juliane and B{\"o}hm, Alexander and Nitschke, J{\"o}rg and Burdukiewicz, Michał and Weinreich, J{\"o}rg and Ali, Aamir and Roggenbuck, Dirk and R{\"o}diger, Stefan and Schierack, Peter}, title = {Genotypic and phenotypic characteristics in association with biofilm formation in different pathotypes of human clinical Escherichia coli isolates}, series = {Applied and Environmental Microbiology}, volume = {83}, journal = {Applied and Environmental Microbiology}, number = {24}, issn = {1098-5336}, doi = {10.1128/AEM.01660-17}, language = {en} } @misc{LiebschRoedigerBoehmetal., author = {Liebsch, Claudia and R{\"o}diger, Stefan and B{\"o}hm, Alexander and Nitschke, J{\"o}rg and Weinreich, J{\"o}rg and Fruth, Angelika and Roggenbuck, Dirk and Lehmann, Werner and Schedler, Uwe and Juretzek, Thomas and Schierack, Peter}, title = {Solid-phase microbead array for multiplex O-serotyping of Escherichia coli}, series = {Microchimica Acta}, volume = {184}, journal = {Microchimica Acta}, number = {5}, issn = {0026-3672}, doi = {10.1007/s00604-017-2088-4}, pages = {1405 -- 1415}, language = {en} }