@misc{DinterJurischkaWeissetal., author = {Dinter, Franziska and Jurischka, Christoph and Weiss, Romano and R{\"o}diger, Stefan}, title = {Multiparametrische Antik{\"o}rperdetektion f{\"u}r die Diagnostik}, series = {BIOspektrum}, volume = {26}, journal = {BIOspektrum}, number = {3}, issn = {1868-6249}, doi = {10.1077/s12268-020-1375-5}, pages = {265 -- 267}, language = {de} } @misc{DinterBurdukiewiczSchieracketal., author = {Dinter, Franziska and Burdukiewicz, Michał and Schierack, Peter and Lehmann, Werner and Nestler, J{\"o}rg and Dame, Gregory and R{\"o}diger, Stefan}, title = {Simultaneous detection and quantification of DNA and protein biomarkers in spectrum of cardiovascular diseases in a microfluidic microbead chip}, series = {Analytical and Bioanalytical Chemistry}, volume = {411}, journal = {Analytical and Bioanalytical Chemistry}, number = {29}, issn = {1618-2650}, doi = {10.1007/s00216-019-02199-x}, pages = {7725 -- 7735}, language = {en} } @misc{JurischkaDinterEfimovaetal., author = {Jurischka, Christoph and Dinter, Franziska and Efimova, Anastasia and Weiss, Romano and Schiebel, Juliane and Schulz, Christian and Fayziev, Bekzodjon and Schierack, Peter and Fischer, Thomas and R{\"o}diger, Stefan}, title = {An explorative study of polymers for 3D printing of bioanalytical test systems}, series = {Clinical Hemorheology and Microcirculation}, volume = {75}, journal = {Clinical Hemorheology and Microcirculation}, number = {1}, issn = {1875-8622}, doi = {10.3233/CH-190713}, pages = {57 -- 84}, abstract = {Background: The 3D printing is relevant as a manufacturing technology of functional models for forensic, pharmaceutical and bioanalytical applications such as drug delivery systems, sample preparation and point-of-care tests. Objective: Melting behavior and autofluorescence of materials are decisive for optimal printing and applicability of the product which are influenced by varying unknown additives. Methods: We have produced devices for bioanalytical applications from commercially available thermoplastic polymers using a melt-layer process. We characterized them by differential scanning calorimetry, fluorescence spectroscopy and functional assays (DNA capture assay, model for cell adhesion, bacterial adhesion and biofilm formation test). Results: From 14 tested colored, transparent and black materials we found only deep black acrylonitrile-butadiene-styrene (ABS) and some black polylactic acid (PLA) useable for fluorescence-based assays, with low autofluorescence only in the short-wave range of 300-400 nm. PLA was suitable for standard bioanalytical purposes due to a glass transition temperature of approximately 60°C, resistance to common laboratory chemicals and easy print processing. For temperature-critical methods, such as hybridization reactions up to 90°C, ABS was better suited. Conclusions: Autofluorescence was not a disadvantage per se but can also be used as a reference signal in assays. The rapid development of individual protocols for sample processing and analysis required the availability of a material with consistent quality over time. For fluorescence-based assays, the use of commercial standard materials did not seem to meet this requirement.}, language = {en} } @misc{DeutschmannDinterRoedigeretal., author = {Deutschmann, Claudia and Dinter, Franziska and R{\"o}diger, Stefan and Roggenbuck, Dirk and Schierack, Peter}, title = {Comparison of Lab and Point of Care (POC) technologies - case study for CHI3L1, Potsdam Days on Bioanalysis}, pages = {1}, language = {en} } @misc{DinterDeutschmannSchieracketal., author = {Dinter, Franziska and Deutschmann, Claudia and Schierack, Peter and Dame, Gregory and R{\"o}diger, Stefan}, title = {Integration of Cardiovascular Disease Biomarkers in a Microfluidic Microbead Chip}, pages = {1}, language = {en} } @misc{AzamRoesslingGeitheetal., author = {Azam, Hafiz Muhammad Husnain and R{\"o}ßling, Rosa Ilse and Geithe, Christiane and Khan, Muhammad Moman and Dinter, Franziska and Hanack, Katja and Pr{\"u}ß, Harald and Husse, Britta and Roggenbuck, Dirk and Schierack, Peter and R{\"o}diger, Stefan}, title = {MicroRNA biomarkers as next-generation diagnostic tools for neurodegenerative diseases: a comprehensive review}, series = {Frontiers in Molecular Neuroscience}, volume = {17}, journal = {Frontiers in Molecular Neuroscience}, publisher = {Frontiers Media S.A.}, issn = {1662-5099}, doi = {10.3389/fnmol.2024.1386735}, pages = {35}, abstract = {Neurodegenerative diseases (NDs) are characterized by abnormalities within neurons of the brain or spinal cord that gradually lose function, eventually leading to cell death. Upon examination of affected tissue, pathological changes reveal a loss of synapses, misfolded proteins, and activation of immune cells—all indicative of disease progression—before severe clinical symptoms become apparent. Early detection of NDs is crucial for potentially administering targeted medications that may delay disease advancement. Given their complex pathophysiological features and diverse clinical symptoms, there is a pressing need for sensitive and effective diagnostic methods for NDs. Biomarkers such as microRNAs (miRNAs) have been identified as potential tools for detecting these diseases. We explore the pivotal role of miRNAs in the context of NDs, focusing on Alzheimer's disease, Parkinson's disease, Multiple sclerosis, Huntington's disease, and Amyotrophic Lateral Sclerosis. The review delves into the intricate relationship between aging and NDs, highlighting structural and functional alterations in the aging brain and their implications for disease development. It elucidates how miRNAs and RNA-binding proteins are implicated in the pathogenesis of NDs and underscores the importance of investigating their expression and function in aging. Significantly, miRNAs exert substantial influence on post-translational modifications (PTMs), impacting not just the nervous system but a wide array of tissues and cell types as well. Specific miRNAs have been found to target proteins involved in ubiquitination or de-ubiquitination processes, which play a significant role in regulating protein function and stability. We discuss the link between miRNA, PTM, and NDs. Additionally, the review discusses the significance of miRNAs as biomarkers for early disease detection, offering insights into diagnostic strategies.}, language = {en} }