@misc{VidalVerduTorrentIglesiasetal., author = {Vidal-Verd{\´u}, {\`A}ngela and Torrent, Daniel and Iglesias, Alba and Latorre-P{\´e}rez, Adriel and Abendroth, Christian and Corb{\´i}n-Agust{\´i}, Paola and Peret{\´o}, Juli and Porcar, Manuel}, title = {The highly differentiated gut of Pachnoda marginata hosts sequential microbiomes: microbial ecology and potential applications}, series = {npj Biofilms and Microbiomes}, volume = {10}, journal = {npj Biofilms and Microbiomes}, number = {1}, publisher = {Springer Science and Business Media LLC}, issn = {2055-5008}, doi = {10.1038/s41522-024-00531-7}, pages = {14}, abstract = {Insect gut microbiomes play a crucial role in the insect development and are shaped, among other factors, by the specialized insect diet habits as well as the morphological structure of the gut. Rose chafers (Pachnoda spp.; Coleoptera: Scarabaeidae) have a highly differentiated gut characterized by a pronounced hindgut dilation which resembles a miniaturized rumen. Specifically, the species Pachnoda marginata has not been previously studied in detail in terms of microbial ecology. Here, we show a fine scale study of the highly compartmentalized gut of P. marginata by using amplicon and metagenomic sequencing to shed light on the bacterial, archaeal and fungal communities thriving in each section of the gut. We found a microbial gradient along the gut from aerobic (foregut) to strictly anaerobic communities (hindgut). In addition, we have characterized interesting biological activities and metabolic pathways of gut microbial communities related to cellulose degradation, methane production and sulfate reduction. Taken together, our results reveal the highly diverse microbial community and the potential of P. marginata gut as a source of industrially relevant microbial diversity.}, language = {en} } @misc{OttoAlipoursarbaniTorrentetal., author = {Otto, Pascal and Alipoursarbani, Mozhdeh and Torrent, Daniel and Latorre-P{\´e}rez, Adriel and Paust, Thomas and Albert, Alfred and Abendroth, Christian}, title = {Microbiome Characterization after Aerobic Digestate Reactivation of Anaerobically Digested Sewage Sludge}, series = {Fermentation}, volume = {9}, journal = {Fermentation}, number = {5}, issn = {2311-5637}, doi = {10.3390/fermentation9050471}, abstract = {A demonstrator plant of a recently patented process for improved sludge degradation has been implemented on a municipal scale. In a 1500 m3 sewage sludge digester, an intermediary stage with aerobic sewage sludge reactivation was implemented. This oxic activation increased the biogas yield by up to 55\% with a 25\% reduction of the remaining fermentation residue volume. Furthermore, this process allowed an NH4-N removal of over 90\%. Additionally, 16S rRNA gene amplicon high-throughput sequencing of the reactivated digestate showed a reduced number of methane-forming archaea compared to the main digester. Multiple ammonium-oxidizing bacteria were detected. This includes multiple genera belonging to the family Chitinophagaceae (the highest values reached 18.8\% of the DNA sequences) as well as a small amount of the genus Candidatus nitrosoglobus (<0.3\%). In summary, the process described here provides an economically viable method to eliminate nitrogen from sewage sludge while achieving higher biogas yields and fewer potential pathogens in the residuals.}, language = {en} } @misc{OttoWitkabelBarthetal., author = {Otto, Pascal and Witkabel, Philipp and Barth, Maximilian and Ammar, Aziz Ben and Rocktaeschel, Benjamin and Torrent, Daniel and Latorre-Per{\´e}z, Adriel and Krause, Maximilian and Abendroth, Christian}, title = {Adaptation of the anaerobic microbiome for in-situ power-to-CH4 processes through fuzzy logic control of H2 input}, series = {Bioresource technology reports}, volume = {31}, journal = {Bioresource technology reports}, publisher = {Elsevier BV}, address = {Amsterdam}, issn = {2589-014X}, doi = {10.1016/j.biteb.2025.102194}, pages = {1 -- 11}, abstract = {This study presents the first application of fuzzy logic control (FLC) for regulating in-situ biological methanation via H2 injection in a fixed-bed reactor. Given the role of CH4 as a storable and infrastructure-compatible energy carrier, the aim was to improve CH4 production by dynamically adapting the H2 supply to the metabolic capacity of the microbiome. This will pave the way for the biological utilisation and storage of renewable H2. Over a 226- day operational period, H2 input was gradually increased based on real-time headspace gas measurements. Coupling the gradual increase in H2 supply with real-time gas composition data allowed the microbiome to adapt dynamically to changing process conditions. This approach led to a 49 \% increase in CH4 concentration, from 58.4 \% to 87.0 \%, and improved H2 conversion rates from 98.2 \% to 99.0 \%. Chemical analyses of COD, organic acids and nitrogen were performed, as well as taxonomic analyses using 16S rRNA sequencing, and multivariate methods were applied to confirm the adaptation of the microbiome to the FLC H2 injection. The adopted microbial community was dominated by strictly hydrogenotrophic methanogens, Methanoculleus and Methanobacterium, while increased abundances of Petrimonas, Rectinema, Syntrophomonas, and Geobacter indicate interspecies H2 transfer and syntrophic cooperation enhancing methanogenesis. These findings demonstrate that FLC-based H2 control enables dynamic adjustment of in-situ methanation, optimising the interaction between hydrogenogenic, fermentative, syntrophic acetate-oxidising, and hydrogenotrophic microorganisms. This work introduces an adaptive control strategy that supports stable and efficient bio-CH4 production and represents a significant advance in the field of power-to-gas technologies.}, language = {en} }