@misc{GeorgeNoackVaneketal., author = {George, Sandra and Noack, M. and Vanek, Monika and Rentzsch, J. and R{\"o}ber, Nadja and Roggenbuck, Dirk and Conrad, Karsten and K{\"u}pper, Jan-Heiner}, title = {Expression of nicotinic acetylcholine receptor subunits in HEp-2 cells for immunodetection of autoantibody specificities in sera fro Myasthenia gravis patients}, series = {Clinical Hemorheology and Microcirculation}, volume = {61}, journal = {Clinical Hemorheology and Microcirculation}, number = {2}, issn = {1875-8622}, doi = {10.3233/CH-151999}, pages = {385 -- 396}, language = {en} } @misc{GeorgePaulickKnuetteretal., author = {George, Sandra and Paulick, Silvia and Kn{\"u}tter, Ilka and R{\"o}ber, Nadja and Hiemann, Rico and Roggenbuck, Dirk and Conrad, Karsten and K{\"u}pper, Jan-Heiner}, title = {Stable Expression of Human Muscle-Specific Kinase in HEp-2 M4 Cells for Automatic Immunofluorescence Diagnostics of Myasthenia Gravis}, series = {PLOS one}, volume = {9}, journal = {PLOS one}, number = {1}, issn = {1932-6203}, doi = {10.1371/journal.pone.0083924}, pages = {e83924}, language = {en} } @misc{GeorgeGeorgiRoggenbucketal., author = {George, Sandra and Georgi, M. and Roggenbuck, Dirk and Conrad, Karsten and K{\"u}pper, Jan-Heiner}, title = {A strategy for cell-based multiplex diagnostics of Myasthenia gravis and autoimmune encephalitis by modifying the subcellular localization of cell membrane autoantigensgravis}, series = {Clinical Hemorheology and Microcirculation}, volume = {58}, journal = {Clinical Hemorheology and Microcirculation}, number = {1}, issn = {1386-0291}, doi = {10.3233/CH-141897}, pages = {211 -- 228}, language = {en} } @incollection{ConradKuepper, author = {Conrad, Karsten and K{\"u}pper, Jan-Heiner}, title = {Tumor-associated autoantibodies}, series = {Autoantibodies}, booktitle = {Autoantibodies}, editor = {Shoenfeld, Yehuda and Meroni, Pier Luigi and Gershwin, M. Eric}, edition = {3rd edition}, publisher = {Elsevier}, address = {Amsterdam [u.a.]}, isbn = {978-0-444-56378-1}, pages = {275 -- 287}, language = {en} } @misc{SowaGrossmannKnuetteretal., author = {Sowa, Mandy and Großmann, Kai and Kn{\"u}tter, Ilka and Hiemann, Rico and R{\"o}ber, Nadja and Anderer, Ursula and Csernok, Elena and Bogdanos, Dimitrios Petrou and Borghi, Maria Orietta and Meroni, Pier Luigi and Schierack, Peter and Reinhold, Dirk and Conrad, Karsten and Roggenbuck, Dirk}, title = {Simultaneous automated screening and confirmatory testing for vasculitis-specific ANCA}, series = {PLoS ONE}, volume = {9}, journal = {PLoS ONE}, number = {9}, issn = {1932-6203}, doi = {10.1371/journal.pone.0107743}, pages = {11}, language = {en} } @misc{KnuetterHiemannBrummaetal., author = {Kn{\"u}tter, Ilka and Hiemann, Rico and Brumma, Therese and B{\"u}ttner, Thomas and Großmann, Kai and Cusini, Marco and Pregnolato, Francesca and Borghi, Maria Orietta and Anderer, Ursula and Conrad, Karsten and Reinhold, Dirk and Roggenbuck, Dirk and Csernok, Elena}, title = {Automated interpretation of ANCA patterns - a new approach in the serology of ANCA-associated vasculitis}, series = {Arthritis Research \& Therapy}, volume = {14}, journal = {Arthritis Research \& Therapy}, issn = {1465-9913}, doi = {10.1186/ar4119}, pages = {10}, language = {en} } @misc{WillitzkiHiemannPetersetal., author = {Willitzki, Annika and Hiemann, Rico and Peters, Vanessa and Sack, Ulrich and Schierack, Peter and R{\"o}diger, Stefan and Anderer, Ursula and Conrad, Karsten and Bogdanos, Dimitrios Petrou and Reinhold, Dirk and Roggenbuck, Dirk}, title = {New PlatformTechnology for Comprehensive Serological Diagnostics of Autoimmune Diseases}, series = {Clinical and Developmental Immunology}, journal = {Clinical and Developmental Immunology}, issn = {1740-2530}, doi = {10.1155/2012/284740}, pages = {1 -- 8}, language = {en} } @misc{NowakMartinGeorgeetal., author = {Nowak, Elisabeth and Martin, F. and George, Sandra and R{\"o}ber, Nadja and Conrad, Karsten and K{\"u}pper, Jan-Heiner}, title = {Bacterial production and purification of immunoreactive paraneoplastic neurological syndrome autoantigen Ma2}, series = {Journal of Cellular Biotechnology}, volume = {2}, journal = {Journal of Cellular Biotechnology}, number = {2}, issn = {2352-3697}, doi = {10.3233/JCB-15029}, pages = {85 -- 91}, abstract = {Paraneoplastic neurological syndromes (PNS) are caused by an immune response against neuronal proteins upon their ectopical expression in tumor cells. Ma2 belongs to the protein family of paraneoplastic Ma antigens (PNMA). Detection of Ma2-specific autoantibodies is relevant for diagnostics of anti-Ma2 PNS and an underlying tumor such as germ testicular cancer, small cell lung cancer or breast cancer. Thus, early tumor treatment should improve the outcome for PNS therapy either. Dot blot immunoassay based on recombinantly expressed and purified autoantigens could offer a sensitive method for identification of paraneoplastic autoantibodies from sera of PNS patients. Here we present purification with IMAC and FPLC of human Ma2 autoantigen upon its recombinant expression in E.coli. Furthermore, we provide evidence that dot blot immunoassays with purified Ma2 autoantigen can be used for detection of Ma2-specific autoantibodies from sera of PNS patients.}, language = {en} } @inproceedings{GeorgeNoackVaneketal., author = {George, Sandra and Noack, Monika and Vanek, Monika and Rentzsch, Juliane and R{\"o}ber, Nadja and Conrad, Karsten and Roggenbuck, Dirk and K{\"u}pper, Jan-Heiner}, title = {Expression of nicotinic acetylcholine receptor subunits in HEp-2 cells for immunodetection of autoantibody specificities in sera from Myasthenia gravis patients}, series = {From autoantibody research to standardized diagnostic assays in the management of human diseases, report on the 12th Dresden Symposium on Autoantibodies, September 23-26, 2015}, booktitle = {From autoantibody research to standardized diagnostic assays in the management of human diseases, report on the 12th Dresden Symposium on Autoantibodies, September 23-26, 2015}, publisher = {Pabst Science Publishers}, address = {Lengerich}, isbn = {978-3-95853-104-8}, pages = {195 -- 196}, language = {en} } @misc{RoggenbuckReinholdWerneretal., author = {Roggenbuck, Dirk and Reinhold, Dirk and Werner, Leal and Schierack, Peter and Bogdanos, Dimitrios Petrou and Conrad, Karsten}, title = {Glycoprotein 2 antibodies in Crohn's disease.}, series = {Advances in Clinical Chemistry}, volume = {60}, journal = {Advances in Clinical Chemistry}, issn = {0065-2423}, pages = {187 -- 208}, language = {en} } @misc{RoggenbuckHiemannSchieracketal., author = {Roggenbuck, Dirk and Hiemann, Rico and Schierack, Peter and Reinhold, Dirk and Conrad, Karsten}, title = {Digital immunofluorescence enables automated detection of antinuclear antibody endpoint titers avoiding serial dilution}, series = {Clinical Chemistry and Laboratory Medicine}, volume = {52}, journal = {Clinical Chemistry and Laboratory Medicine}, number = {2}, issn = {1437-4331}, doi = {10.1515/cclm-2013-0543}, pages = {e9 -- e11}, language = {en} } @misc{WillitzkiLorenzHiemannetal., author = {Willitzki, Annika and Lorenz, Sebastian and Hiemann, Rico and Guttek, Karina and Goihl, Alexander and Hartig, Roland and Conrad, Karsten and Feist, Eugen and Sack, Ulrich and Schierack, Peter and Heiserich, Lisa and Eberle, Caroline and Peters, Vanessa and Roggenbuck, Dirk and Reinhold, Dirk}, title = {Fully automated analysis of chemically induced γH2AX foci in human peripheral blood mononuclear cells by indirect immunofluorescence}, series = {Cytometry Part A}, volume = {83}, journal = {Cytometry Part A}, number = {11}, issn = {1552-4930}, doi = {10.1002/cyto.a.22350}, pages = {1017 -- 1026}, language = {en} } @misc{GrossmannRoggenbuckSchroederetal., author = {Großmann, Kai and Roggenbuck, Dirk and Schr{\"o}der, Christian and Conrad, Karsten and Schierack, Peter and Sack, Ulrich}, title = {Multiplex assessment of non-organ-specific autoantibodies with a novel microbead-based immunoassay}, series = {Cytometry Part A}, volume = {79}, journal = {Cytometry Part A}, number = {2}, issn = {1552-4922}, doi = {10.1002/cyto.a.21009}, pages = {118 -- 125}, language = {en} } @misc{SowaKolendaBaumgartetal., author = {Sowa, Mandy and Kolenda, Rafał and Baumgart, Daniel C. and Pratschke, Johann and Papp, Maria and Tornai, Tamas and Suchanski, Jaroslaw and Bogdanos, Dimitrios Petrou and Mytilinaiou, Maria G. and Hammermann, Jutta and Laass, Martin W. and Conrad, Karsten and Schramm, Christoph and Franke, Andre and Roggenbuck, Dirk and Schierack, Peter}, title = {Mucosal Autoimmunity to Cell-Bound GP2 Isoforms Is a Sensitive Marker in PSC and Associated With the Clinical Phenotype}, series = {Frontiers in Immunology}, volume = {9}, journal = {Frontiers in Immunology}, number = {1959}, issn = {1664-3224}, doi = {10.3389/fimmu.2018.01959}, language = {en} } @misc{RoggenbuckReinholdSchieracketal., author = {Roggenbuck, Dirk and Reinhold, Dirk and Schierack, Peter and Bogdanos, Dimitrios Petrou and Conrad, Karsten and Laass, Martin W.}, title = {Crohn's disease specific pancreatic antibodies: clinical and pathophysiological challenges}, series = {Clinical Chemistry and Laboratory Medicine}, volume = {52}, journal = {Clinical Chemistry and Laboratory Medicine}, number = {4}, issn = {1437-4331}, doi = {10.1515/cclm-2013-0801}, pages = {494}, language = {en} } @misc{SowaGrossmannScholzetal., author = {Sowa, Mandy and Großmann, Kai and Scholz, Juliane and R{\"o}ber, Nadja and R{\"o}diger, Stefan and Schierack, Peter and Conrad, Karsten and Roggenbuck, Dirk and Hiemann, Rico}, title = {Der CytoBead-Assay - Eine neue M{\"o}glichkeit der multiparametrischen Autoantik{\"o}rperanalytik bei systemischen Autoimmunerkrankungen}, series = {Journal of Laboratory Medicine}, volume = {38}, journal = {Journal of Laboratory Medicine}, number = {6}, doi = {10.1515/labmed-2014-0041}, pages = {309 -- 317}, language = {de} } @misc{ScholzGrossmannKnuetteretal., author = {Scholz, Juliane and Großmann, Kai and Kn{\"u}tter, Ilka and Hiemann, Rico and Sowa, Mandy and R{\"o}ber, Nadja and R{\"o}diger, Stefan and Schierack, Peter and Reinhold, Dirk and Bogdanos, Dimitrios Petrou and Meroni, Pier Luigi and Radice, Antonella and Conrad, Karsten and Roggenbuck, Dirk}, title = {Second generation analysis of antinuclear antibody (ANA) by combination of screening and confirmatory testing}, series = {Clinical Chemistry and Laboratory Medicine}, volume = {53}, journal = {Clinical Chemistry and Laboratory Medicine}, number = {12}, issn = {1437-4331}, doi = {10.1515/cclm-2015-0083}, pages = {1991 -- 2002}, language = {en} } @misc{GrossmannRoeberHiemannetal., author = {Großmann, Kai and R{\"o}ber, Nadja and Hiemann, Rico and R{\"o}diger, Stefan and Schierack, Peter and Reinhold, Dirk and Laass, Martin W. and Conrad, Karsten and Roggenbuck, Dirk}, title = {Simultaneous detection of celiac disease-specific IgA antibodies and total IgA}, series = {Autoimmunity Highlights}, volume = {7}, journal = {Autoimmunity Highlights}, number = {2}, issn = {2038-0305}, doi = {10.1007/s13317-016-0073-2}, pages = {10}, language = {en} } @misc{RoggenbuckReinholdBaumgartetal., author = {Roggenbuck, Dirk and Reinhold, Dirk and Baumgart, Daniel C. and Schierack, Peter and Conrad, Karsten and Laass, Martin W.}, title = {Autoimmunity in Crohn's Disease-A Putative Stratification Factor of the Clinical Phenotype}, series = {Advances in Clinical Chemistry}, volume = {77}, journal = {Advances in Clinical Chemistry}, issn = {0065-2423}, doi = {10.1016/bs.acc.2016.06.002}, pages = {77 -- 101}, language = {en} } @misc{SowaHiemannSchieracketal., author = {Sowa, Mandy and Hiemann, Rico and Schierack, Peter and Reinhold, Dirk and Conrad, Karsten and Roggenbuck, Dirk}, title = {Next-Generation Autoantibody Testing by Combination of Screening and Confirmation-the CytoBead® Technology}, series = {Clinical Reviews in Allergy \& Immunology}, volume = {53}, journal = {Clinical Reviews in Allergy \& Immunology}, number = {1}, issn = {1559-0267}, doi = {10.1007/s12016-016-8574-3}, pages = {87 -- 104}, language = {en} } @misc{DeutschmannSowaMurugaiyanetal., author = {Deutschmann, Claudia and Sowa, Mandy and Murugaiyan, Jayaseelan and Roessler, Uwe and R{\"o}ber, Nadja and Conrad, Karsten and Laass, Martin W. and Bogdanos, Dimitrios Petrou and Sipeki, Nora and Papp, Maria and R{\"o}diger, Stefan and Roggenbuck, Dirk and Schierack, Peter}, title = {Identification of Chitinase-3-Like Protein 1 as a Novel Neutrophil Antigenic Target in Crohn's Disease}, series = {Journal of Crohn's and Colitis}, volume = {13}, journal = {Journal of Crohn's and Colitis}, number = {7}, issn = {1876-4479}, doi = {10.1093/ecco-jcc/jjz012}, pages = {894 -- 904}, abstract = {Background and Aims There is an increasing incidence of inflammatory bowel disease [IBD]. Autoimmune responses are involved in the pathophysiology of IBD, but their underlying pathways and target antigens have not yet been fully elucidated. Methods Autoantigenic targets in IBD were identified after separation of whole cell proteins isolated from neutrophils using two-dimensional electrophoresis and matrix assisted laser desorption ionization - time of flight mass spectrometry-based protein identification of the spots that displayed Western blotting signals with anti-neutrophil cytoplasmic antibody-positive sera. The prevalence of IgG, IgA and secretory IgA [sIgA] to chitinase 3-like protein 1 [CHI3L1] was analysed by enzyme-linked immunosorbent assays using recombinant CHI3L1 in 110 patients with Crohn's disease [CD], 95 with ulcerative colitis [UC], 126 with coeliac disease [CeD] and 86 healthy controls [HCs]. Results The 18-glycosylhydrolase family member CHI3L1 was identified as a neutrophil autoantigenic target. CD patients displayed significantly higher levels of IgG to CHI3L1 than patients with UC and CeD (p < 0.0001, respectively). IgA and sIgA to CHI3L1 was significantly higher in CD than in UC, CeD and HCs [p < 0.0001, respectively]. IgA and sIgA to CHI3L1 demonstrated the highest prevalence in CD [25.5\%, 28/110; and 41.8\%\%, 46/110] compared to HCs [2.3\%, 2/86; and 4.7\%\%, 4/86; p = 0.0015 and p < 0.0001] and are associated with a more complicated progression of CD. Conclusion CHI3L1 is a novel neutrophil autoantigenic target in CD. IgA and sIgA to CHI3L1 may serve as novel markers for CD and may facilitate the serological diagnosis of IBD.}, language = {en} }