@misc{MaussNakovWenzeletal., author = {Mauß, Fabian and Nakov, Galin and Wenzel, Paul and Steiner, R{\"u}diger and Kr{\"u}ger, Christian and Zhang, Yongzeh and Rawat, Rajesh and Borg, Andreas and Perlman, Cathleen and Fr{\"o}jd, Karin and Lehtiniemi, Harry}, title = {Soot Simulation under Diesel Engine Conditions Using a Flamelet Approach}, series = {SAE International Journal of Engines}, volume = {2}, journal = {SAE International Journal of Engines}, number = {2}, issn = {1946-3936}, pages = {89 -- 104}, language = {en} } @incollection{UlrichSchlegelMertensetal., author = {Ulrich, Rainer G. and Schlegel, Matthias and Mertens, Marc and Groschup, Martin H. and Schmidt-Chanasit, Jonas and Plenge-B{\"o}nig, Anita and Jacob, Jens and Pelz, Hans-Joachim and Freise, Jona and Wenk, Matthias and Thiel, J{\"o}rg and Triebenbacher, Cornelia and Schmolz, Eric and Kurth, Andreas and Kr{\"u}ger, Frank and R{\"u}he, Ferdinand and Kiffner, Christian and Ansorge, Hermann and Gerwin, Werner and Wegener, Wolfgang and M{\"u}ller, J{\"o}rg and Bemmann, Margit and Wolf, Ronny and Otto, Lutz-Florian and Oehme, Rainer and Pfeffer, Martin and Heckel, Gerald and Schex, Susanne and Essbauer, Sandra S.}, title = {Netzwerk Nagetier-{\"u}bertragene Pathogene: Monitoring von Hantavirus-Infektionen in Deutschland}, series = {Wildhygiene, Wildtierkrankheiten, Parasiten, Epidemiologie}, booktitle = {Wildhygiene, Wildtierkrankheiten, Parasiten, Epidemiologie}, publisher = {Ges. f{\"u}r Wildtier- und Jagdforschung}, address = {Halle/Saale}, isbn = {978-378-88131-2-3}, issn = {1436-3895}, pages = {229 -- 250}, language = {de} } @incollection{FischerKrautzWenskeetal., author = {Fischer, Ulrich and Krautz, Hans Joachim and Wenske, Michael and Tannert, Daniel and Kr{\"u}ger, Perco and Ziems, Christian and Stolten, Detlef and Emonts, Bernd}, title = {Hydrogen Hybrid Power Plant in Prenzlau, Brandenburg}, series = {Hydrogen Science and Engineering - Materials, Processes, Systems and Technology, Bd. 2}, booktitle = {Hydrogen Science and Engineering - Materials, Processes, Systems and Technology, Bd. 2}, publisher = {Wiley-VCH}, address = {Weinheim}, isbn = {978-3-5273323-8-0}, pages = {1033 -- 1052}, language = {en} } @misc{SchulzKruegerGengeLendleinetal., author = {Schulz, Christian and Kr{\"u}ger-Genge, Anne and Lendlein, Andreas and K{\"u}pper, Jan-Heiner and Jung, Friedrich}, title = {Potential Effects of Nonadherent on Adherent Human Umbilical Venous Endothelial Cells in Cell Culture}, series = {International Journal of Molecular Science}, volume = {22}, journal = {International Journal of Molecular Science}, number = {3}, doi = {10.3390/ijms22031493}, pages = {15}, abstract = {The adherence and shear-resistance of human umbilical venous endothelial cells (HUVEC) on polymers is determined in vitro in order to qualify cardiovascular implant materials. In these tests, variable fractions of HUVEC do not adhere to the material but remain suspended in the culture medium. Nonadherent HUVEC usually stop growing, rapidly lose their viability and can release mediators able to influence the growth and function of the adherent HUVEC. The aim of this study was the investigation of the time dependent behaviour of HUVEC under controlled nonadherent conditions, in order to gain insights into potential influences of these cells on their surrounding environment in particular adherent HUVEC in the context of in vitro biofunctionality assessment of cardiovascular implant materials. Data from adherent or nonadherent HUVEC growing on polystyrene-based cell adhesive tissue culture plates (TCP) or nonadhesive low attachment plates (LAP) allow to calculate the number of mediators released into the culture medium either from adherent or nonadherent cells. Thus, the source of the inflammatory mediators can be identified. For nonadherent HUVEC, a time-dependent aggregation without further proliferation was observed. The rate of apoptotic/dead HUVEC progressively increased over 90\% within two days. Concomitant with distinct blebbing and loss of membrane integrity over time, augmented releases of prostacyclin (PGI2, up to 2.91 ± 0.62 fg/cell) and platelet-derived growth factor BB (PDGF-BB, up to 1.46 ± 0.42 fg/cell) were detected. The study revealed that nonadherent, dying HUVEC released mediators, which can influence the surrounding microenvironment and thereby the results of in vitro biofunctionality assessment of cardiovascular implant materials. Neglecting nonadherent HUVEC bears the risk for under- or overestimation of the materials endothelialization potential, which could lead to the loss of relevant candidates or to uncertainty with regard to their suitability for cardiac applications. One approach to minimize the influence from nonadherent endothelial cells could be their removal shortly after observing initial cell adhesion. However, this would require an individual adaptation of the study design, depending on the properties of the biomaterial used.}, language = {en} } @misc{KruegerGengeJungKuepperetal., author = {Kr{\"u}ger-Genge, Anne and Jung, Friedrich and K{\"u}pper, Jan-Heiner and Lehmann, Christian and Franke, Ralf-Peter}, title = {Actin type and distribution in erythrocytes}, series = {Journal of Cellular Biotechnology}, volume = {3}, journal = {Journal of Cellular Biotechnology}, number = {2}, issn = {2352-3697}, doi = {10.3233/JCB-179014}, pages = {81 -- 83}, abstract = {Erythrocytes transport oxygen from the lungs to the tissues. The excess surface area together with the elasticity of the erythrocyte cell membrane provides the flexibility needed to pass through the microvasculature where the oxygen exchange occurs. Although the architecture of the red cell and its membrane-associated cytoskeletal network is known in general, the factors that control the characteristic shape change during echinocyte formation are poorly understood. In this short report we show that in echinocytes a completely reorganized membrane cytoskeleton with a box-like structure of actin filaments prevailed indicating the importance of the actin cytoskeleton during echinocyte formation.}, language = {en} } @misc{KruegerGengeKoehlerLaubeetal., author = {Kr{\"u}ger-Genge, Anne and K{\"o}hler, Susanne and Laube, Markus and Haileka, Vanessa and Lemm, Sandy and Majchrzak, Karolina and Kammerer, Sarah and Schulz, Christian and Storsberg, Joachim and Pietzsch, Jens and K{\"u}pper, Jan-Heiner and Jung, Friedrich}, title = {Anti-Cancer Prodrug Cyclophosphamide Exerts Thrombogenic Effects on Human Venous Endothelial Cells Independent of CYP450 Activation—Relevance to Thrombosis}, series = {Cells}, volume = {12}, journal = {Cells}, number = {15}, issn = {2073-4409}, doi = {10.3390/cells12151965}, abstract = {Cancer patients are at a very high risk of serious thrombotic events, often fatal. The causes discussed include the detachment of thrombogenic particles from tumor cells or the adverse effects of chemotherapeutic agents. Cytostatic agents can either act directly on their targets or, in the case of a prodrug approach, require metabolization for their action. Cyclophosphamide (CPA) is a widely used cytostatic drug that requires prodrug activation by cytochrome P450 enzymes (CYP) in the liver. We hypothesize that CPA could induce thrombosis in one of the following ways: (1) damage to endothelial cells (EC) after intra-endothelial metabolization; or (2) direct damage to EC without prior metabolization. In order to investigate this hypothesis, endothelial cells (HUVEC) were treated with CPA in clinically relevant concentrations for up to 8 days. HUVECs were chosen as a model representing the first place of action after intravenous CPA administration. No expression of CYP2B6, CYP3A4, CYP2C9 and CYP2C19 was found in HUVEC, but a weak expression of CYP2C18 was observed. CPA treatment of HUVEC induced DNA damage and a reduced formation of an EC monolayer and caused an increased release of prostacyclin (PGI2) and thromboxane (TXA) associated with a shift of the PGI2/TXA balance to a prothrombotic state. In an in vivo scenario, such processes would promote the risk of thrombus formation.}, language = {en} } @misc{JungMoranEngeletal., author = {Jung, Ernst Michael and Moran, Valentina Oca{\~n}aa and Engel, Martin and Kr{\"u}ger-Genge, Anne and Stroszczynski, Christian and Jung, Friedrich}, title = {Modified contrast-enhanced ultrasonography with the new high-resolution examination technique of high frame rate contrast-enhanced ultrasound (HiFR-CEUS) for characterization of liver lesions: First results}, series = {Clinical Hemorheology and Microcirculation}, journal = {Clinical Hemorheology and Microcirculation}, issn = {1875-8622}, doi = {10.3233/CH-221449}, pages = {1 -- 16}, language = {en} } @misc{SchulzKruegerGengeJungetal., author = {Schulz, Christian and Kr{\"u}ger-Genge, Anne and Jung, Friedrich and Lendlein, Andreas}, title = {Aptamer supported in vitro endothelialization of poly(ether imide) films}, series = {Clinical Hemorheology and Microcirculation}, volume = {75}, journal = {Clinical Hemorheology and Microcirculation}, number = {2}, issn = {1875-8622}, doi = {10.3233/CH-190775}, pages = {201 -- 217}, abstract = {Implantation of synthetic small-diameter vascular bypass grafts is often associated with an increased risk of failure, due to thrombotic events or late intimal hyperplasia. As one of the causes an insufficient hemocompatibility of the artificial surface is discussed. Endothelialization of synthetic grafts is reported to be a promising strategy for creating a self-renewing and regulative anti-thrombotic graft surface. However, the establishment of a shear resistant cell monolayer is still challenging. In our study, cyto- and immuno-compatible poly(ether imide) (PEI) films were explored as potential biomaterial for cardiovascular applications. Recently, we reported that the initial adherence of primary human umbilical vein endothelial cells (HUVEC) was delayed on PEI-films and about 9 days were needed to establish a confluent and almost shear resistant HUVEC monolayer. To accelerate the initial adherence of HUVEC, the PEI-film surface was functionalized with an aptamer-cRGD peptide based endothelialization supporting system. With this functionalization the initial adherence as well as the shear resistance of HUVEC on PEI-films was considerable improved compared to the unmodified polymer surface. The in vitro results confirm the general applicability of aptamers for an efficient functionalization of substrate surfaces.}, language = {en} } @misc{WenzelGezginSteineretal., author = {Wenzel, Paul and Gezgin, A. and Steiner, R{\"u}diger and Kr{\"u}ger, Christian and Netzell, Karl and Lehtiniemi, Harry and Mauß, Fabian}, title = {Modeling of the soot particle size distribution in diesel engines}, series = {Conference proceedings, Conference on Thermo- and Fluid Dynamic Processes in Diesel Engines, September 12th - 15th 2006, Valencia, Spain}, journal = {Conference proceedings, Conference on Thermo- and Fluid Dynamic Processes in Diesel Engines, September 12th - 15th 2006, Valencia, Spain}, publisher = {Univ. Polit{\´e}cnica}, address = {Valencia}, isbn = {84-9705-982-4}, pages = {397 -- 410}, language = {en} } @inproceedings{ZiemsTannertTillmannetal., author = {Ziems, Christian and Tannert, Daniel and Tillmann, Christine and Kr{\"u}ger, Perco and Krause, S. and Krautz, Hans Joachim}, title = {Untersuchungen zur fortschrittlichen alkalischen Druckelektrolyse am Wasserstoff-Forschungszentrum Cottbus}, language = {de} }