@misc{OttoAbendrothDornack, author = {Otto, Pascal and Abendroth, Christian and Dornack, Christina}, title = {Designer-Mikrobiome f{\"u}r den Biogassektor}, series = {M{\"u}ll und Abfall}, volume = {54}, journal = {M{\"u}ll und Abfall}, number = {05}, issn = {1863-9763}, doi = {10.37307/j.1863-9763.2022.05.05}, pages = {243 -- 247}, abstract = {Das Institut f{\"u}r Abfall- und Kreislaufwirtschaft (IAK) der TU Dresden ist am von der Europ{\"a}ischen Union im Rahmen des H2020-Programms gef{\"o}rderten Projekts "Micro4Biogas" beteiligt. Ziel des Projektes ist es, zur Unterst{\"u}tzung und Modernisierung des Biogassektors in den EU-L{\"a}ndern beizutragen. Im Mittelpunkt stehen die Untersuchung der anaeroben Verg{\"a}rung und des Mikrobioms, welche trotz jahrzehntelanger Forschung nicht ausreichend entschl{\"u}sselt sind. Obwohl das Wissen {\"u}ber die beteiligten Mikroorganismen zunimmt, wird das Mikrobiom nach wie vor als "Black Box" betrachtet. Im Rahmen von Micro4Biogas erfolgt die Charakterisierung nat{\"u}rlicher mikrobieller Gemeinschaften sowie die Erforschung neuer synthetischer mikrobieller Gemeinschaften. Darauf aufbauend erfolgt die Entwicklung von Strategien zur gezielten Beeinflussung des Mikrobioms (Bioaugmentation), die dazu dienen, den Prozess robuster, schneller, produktiver und standardisierter zu gestalten. Das bestehende und generierte Fachwissen wird in einer frei verf{\"u}gbaren Roadmap zusammengefasst. Die Roadmap soll zudem Leitlinien und Handlungsempfehlungen f{\"u}r Industrie und Politik zur nachhaltigen und optimierten Produktion von Biogas enthalten.}, language = {de} } @misc{OttoAbendroth, author = {Otto, Pascal and Abendroth, Christian}, title = {The European Biogas Landscape: One of the most comprehensive studies on the biogas sector}, address = {Leipzig}, abstract = {At the BIOENERGY DOC2022 (5th doctoral colloquium bioenergy), results from the currently running EU project Micro4Biogas were presented (https://micro4biogas.eu/). The presented poster describes the sampling of more than 70 samples from a large number of biogas plants in Germany and the Netherlands. Samples were analysed chemically and taxonomically. A comprehensive dataset was developed for the technical description of the respective plants. In the next step, a holistic, multivariate analysis of the collected data sets is planned.}, language = {en} } @misc{AbendrothDornackNefigmannetal., author = {Abendroth, Christian and Dornack, Christina and Nefigmann, Sven and Otto, Pascal}, title = {Mikroskopische Helden der Biogasbranche}, series = {Tagungsband zum 20. Dialog Abfallwirtschaft und zum 16. Rostocker Bioenergieforum}, journal = {Tagungsband zum 20. Dialog Abfallwirtschaft und zum 16. Rostocker Bioenergieforum}, editor = {Nelles, Michael}, address = {Rostock}, isbn = {978-3-86009-535-5}, doi = {10.18453/rosdok_id00003615}, pages = {233 -- 241}, abstract = {Das Streben nach umweltfreundlicher Energie und Wertstoffen hat in den letzten Jahren, gest{\"a}rkt durch Wirtschaft und Politik, den Drang nach Innovation erheblich angeregt. Dieser Entwicklungsdrang findet sich in vielen Bereichen wieder, unter anderem in der Bio{\"o}konomie und hier auch bei Biogasanlagen. Das Schlagwort „Mikrobiom" ist im medizinischen Bereich bereits weit verbreitet und wird im popul{\"a}rwissenschaftlichen Bereich in zahlreichen B{\"u}chern diskutiert. Dies unterstreicht das große gesellschaftliche Interesse an diesem Thema. Neben diesen Erkenntnissen gewinnt das Mikrobiom im Bereich der Bioenergien zunehmend an Bedeutung. In den letzten Jahren sind in hochrangigen, internationalen Fachjournalen zahlreichen Artikel erschienen, welche aufzeigen, dass Biogasanlagen wahre Superhelden unter den Mikroorganismen beherbergen. Die hohe Diversit{\"a}t wurde bereits in zahlreichen Publikationen diskutiert. Trotz dessen wird das Mikrobiom als „Blackbox" betrachtet. Wissenschaftler weltweit versuchen diese Blackbox aufzul{\"o}sen und hierbei traten einige {\"U}berraschungen auf. Dieses neue Wissen k{\"o}nnte den Grundstein f{\"u}r neue Konzepte der Prozesssteuerung legen. Ziel der vorliegenden Arbeit ist in diesem Zusammenhang eine Kurz{\"u}bersicht {\"u}ber mikrobielle Innovation im Zusammenhang mit anaeroben Mikrobiomen.}, language = {de} } @misc{Abendroth, author = {Abendroth, Christian}, title = {Zirkul{\"a}re Mikrobiome f{\"u}r Recycling- und R{\"u}ckgewinnungsanwendungen}, series = {Werkstoffe in der Fertigung}, journal = {Werkstoffe in der Fertigung}, number = {1}, issn = {0939-2629}, pages = {27 -- 28}, abstract = {Der Artikel stellt das Fachgebiet der Kreislaufwirtschaft an der BTU vor. Forschungsthemen des Fachgebietes werden kurz erl{\"a}utert. Diese umfassen Biolaugung zur Behandlung von Leiterplatten, anaerobe Fermentation als Multiproduktplattform, Power2Gas Prozesse sowie das Verst{\"a}ndnis mikrobieller Gemeinschaften mit Bedeutung f{\"u}r die Kreislaufwirtschaft.}, language = {de} } @misc{JansenAbendroth, author = {Jansen, Nils and Abendroth, Christian}, title = {Unveiling the Biochar Potential: Lowering Greenhouse Gas Emissions across the Biogas Supply Chain}, series = {Kreislauf und Ressourcenwirtschaft : 13.Wissenschaftskongress : am 15. und 16. Februar 2024 an der Technischen Universit{\"a}t Wien, Tagungsband}, journal = {Kreislauf und Ressourcenwirtschaft : 13.Wissenschaftskongress : am 15. und 16. Februar 2024 an der Technischen Universit{\"a}t Wien, Tagungsband}, editor = {Bockreis, Anke and Faulstich, Martin and Flamme, Sabine and Kranert, Martin and Mocker, Mario and Nelles, Michael and Quicker, Peter and Rettenberger, Gerhard and Rotter, Vera Susanne}, publisher = {Innsbruck university press}, address = {Innsbruck}, isbn = {978-3-99106-120-5}, doi = {10.15203/99106-120-5}, pages = {279 -- 286}, abstract = {The carbon-rich material known as biochar is the product of pyrolysis - a thermochemical conversion of biomass in the absence of oxygen. Given its characteristics, including long-term stability and sorption capacity, biochar has received increasing attention as a promising tool for addressing climate change and enhancing the production of biogas. This work combines both functions by examining, through different application scenarios, the potential role of biochar in mitigating greenhouse gas (GHG) emissions across the biogas supply chain. The wood-derived and high pyrolyzed biochar CarboFerm® was used as an example biochar for application in four different scenarios: (1) Application to soil without fertilizer, (2) application to soil with fertilizer, (3) application to biogas fermenter, (4) application to digestate treatment. The GHG mitigation was calculated by applying the methodology of the Renewable Energy Directive II (RED II). The scenario comparison resulted in GHG mitigation effectiveness of 0.03, 0.01, 0.06, and 0.03 g CO2eq / MJ biogas / t biochar, respectively. Thus, this study recommends using biochar in biogas fermenters and subsequently applying the biochar-enriched digestate to agricultural soil to reduce GHG emissions from biogas production. Furthermore, it is suggested that the mitigation potential of biochar may not be fully recognized in the RED, potentially resulting in an underestimation of its effectiveness.}, language = {en} } @misc{AlipoursarbaniAbendroth, author = {Alipoursarbani, Mozhdeh and Abendroth, Christian}, title = {Aerobic Digestate Reactivation of Anaerobically Digested Sewage Sludge - A Micro Review}, series = {Kreislauf und Ressourcenwirtschaft : 13.Wissenschaftskongress : am 15. und 16. Februar 2024 an der Technischen Universit{\"a}t Wien}, journal = {Kreislauf und Ressourcenwirtschaft : 13.Wissenschaftskongress : am 15. und 16. Februar 2024 an der Technischen Universit{\"a}t Wien}, editor = {Bockreis, Anke and Faulstich, Martin and Flamme, Sabine and Kranert, Martin and Mocker, Mario and Nelles, Michael and Quicker, Peter and Rettenberger, Gerhard and Rotter, Vera Susanne}, publisher = {Innsbruck university press}, address = {Innsbruck}, isbn = {978-3-99106-120-5}, doi = {10.15203/99106-120-5}, pages = {177 -- 180}, abstract = {The authors of the present work summarize their recently published article "Microbiome Characterization after Aerobic Digestate Reactivation of Anaerobically Digested Sewage Sludge" from Otto et al. (2023). This article presents the outcomes of a municipal-scale demonstrator plant implementing a recently patented process for improved sludge degradation within a municipal sewage treatment system. The study focused on a 1500 m³ sewage sludge digester, where an intermediate aerobic sewage sludge reactivation stage was introduced. This reactivation resulted in a 55\% increase in biogas production and a 25\% reduction in residual solids. For the residual NH4 in the liquid phase of the sewage sludge, the process achieved NH4-N removal rates exceeding 90\%. To gain insights into the microbial community dynamics, 16S rRNA gene amplicon high-throughput sequencing was employed on the reactivated digestate. The analysis revealed a reduced population of methane-forming archaea compared to the primary digester, along with the detection of multiple ammonium-oxidizing bacteria. Notably, genera within the Chitinophagaceae family, comprising up to 18.8\% of DNA sequences, were prevalent, alongside a small presence of Candidatus nitrosoglobus (<0.3\%). The study highlights an economically viable approach for efficient nitrogen removal from sewage sludge while simultaneously increasing biogas yields and reducing potential pathogens in the residual material.}, language = {en} } @misc{WitkabelAbendroth, author = {Witkabel, Philipp and Abendroth, Christian}, title = {A systematic literature review of microbial anammox consortia in UASB/ EGSB-reactors}, series = {Chemosphere}, volume = {367}, journal = {Chemosphere}, publisher = {Elsevier BV}, issn = {0045-6535}, doi = {10.1016/j.chemosphere.2024.143630}, pages = {17}, abstract = {Anaerobic ammonium oxidation (anammox) poses an emerging research field as it can outstand previous processes of biological wastewater treatment in terms of efficiency and costs. Anammox bacteria have the ability to metabolise NH4+ and NO2- to produce N2 under anaerobic conditions. Despite numerous studies, there is a lack of research on the co-occurrence and interrelationship of the predominant microbes that inhabit anammox-related processes. This systematic literature review follows the PSALSAR approach to assess metagenomic data on anammox bacteria and functional microbes in upstream reactors. Essential information on the physiology, metabolic pathways and inhibitory effects of anammox bacteria are reviewed and functional bacteria such as ammonia-oxidising bacteria (AOB), nitrite-oxidising bacteria (NOB), ammonia-oxidising Archaea (AOA) and denitrifying bacteria are identified. Candidatus Kuenenia and Candidatus Brocadia were the most frequently sequenced genera in the observed literature. Pseudomonadota, Chloroflexota and Bacteroidota were prevalent regardless of crucial operational parameters and configurations that affect the microbial community. Interrelationship analysis revealed a positive association between the versatility of a phylum's metabolism and its presence in the observed wastewater treatment literature. Several groups, such as Calditrichota, Myxococcota and Deinococcota are highly underrepresented, a finding that should be investigated in more detail. No evidence was found to suggest that high anammox ratios are correlated with high nitrogen removal efficiencies, as some studies found high efficiency despite low anammox abundance (<1\%).}, language = {en} } @misc{OttoPucholRoyoOrtegaLegarretaetal., author = {Otto, Pascal and Puchol-Royo, Roser and Ortega-Legarreta, Asier and Tanner, Kristie and Tideman, Jeroen and De Vries, Sjoerd-Jan and Pascual, Javier and Porcar, Manuel and Latorre-P{\´e}rez, Adriel and Abendroth, Christian}, title = {Multivariate comparison of taxonomic, chemical and operational data from 80 different full-scale anaerobic digester-related systems}, series = {Biotechnology for Biofuels and Bioproducts}, volume = {17}, journal = {Biotechnology for Biofuels and Bioproducts}, doi = {10.1186/s13068-024-02525-1}, pages = {1 -- 17}, abstract = {Background: The holistic characterization of different microbiomes in anaerobic digestion (AD) systems can contribute to a better understanding of these systems and provide starting points for bioengineering. The present study investigates the microbiome of 80 European full-scale AD systems. Operational, chemical and taxonomic data were thoroughly collected, analysed and correlated to identify the main drivers of AD processes. Results: The present study describes chemical and operational parameters for a broad spectrum of different AD systems. With this data, Spearman correlation and differential abundance analyses were applied to narrow down the role of the individual microorganisms detected. The authors succeeded in further limiting the number of microorganisms in the core microbiome for a broad range of AD systems. Based on 16S rRNA gene amplicon sequencing, MBA03, Proteiniphilum, a member of the family Dethiobacteraceae, the genus Caldicoprobacter and the methanogen Methanosarcina were the most prevalent and abundant organisms identified in all digesters analysed. High ratios for Methanoculleus are often described for agricultural co-digesters. Therefore, it is remarkable that Methanosarcina was surprisingly high in several digesters reaching ratios up to 47.2\%. The various statistical analyses revealed that the microorganisms grouped according to different patterns. A purely taxonomic correlation enabled a distinction between an acetoclastic cluster and a hydrogenotrophic one. However, in the multivariate analysis with chemical parameters, the main clusters corresponded to hydrolytic and acidogenic microorganisms, with SAOB bacteria being particularly important in the second group. Including operational parameters resulted in digester-type specific grouping of microbes. Those with separate acidification stood out among the many reactor types due to their unexpected behaviour. Despite maximizing the organic loading rate in the hydrolytic pretreatments, these stages turned into extremely robust methane production units. Conclusions: From 80 different AD systems, one of the most holistic data sets is provided. A very distinct formation of microbial clusters was discovered, depending on whether taxonomic, chemical or operational parameters were combined. The microorganisms in the individual clusters were strongly dependent on the respective reference parameters.}, language = {en} } @misc{OttoAbendroth, author = {Otto, Pascal and Abendroth, Christian}, title = {Neue Bakterienordnung entdeckt}, series = {Biogas Journal}, volume = {2024}, journal = {Biogas Journal}, number = {2}, issn = {1619-8913}, pages = {96 -- 99}, abstract = {Wissenschaftler*innen des europ{\"a}ischen Forschungsprojektes Micro4Biogas haben eine neue taxonomische Ordnung von Bakterien entdeckt und klassifiziert, die auf die Zersetzung von organischem Material spezialisiert sind und der Schl{\"u}ssel zu einer optimierten Biogasproduktion sein k{\"o}nnten. Die von ihnen als Darwinibacteriales bezeichnete Ordnung ist eine der am h{\"a}ufigsten vorkommenden Bakterien-Ordnungen in Biogasanlagen, wurde aber bisher noch nie wissenschaftlich klassifiziert.}, language = {de} } @misc{VidalVerduTorrentIglesiasetal., author = {Vidal-Verd{\´u}, {\`A}ngela and Torrent, Daniel and Iglesias, Alba and Latorre-P{\´e}rez, Adriel and Abendroth, Christian and Corb{\´i}n-Agust{\´i}, Paola and Peret{\´o}, Juli and Porcar, Manuel}, title = {The highly differentiated gut of Pachnoda marginata hosts sequential microbiomes: microbial ecology and potential applications}, series = {npj Biofilms and Microbiomes}, volume = {10}, journal = {npj Biofilms and Microbiomes}, number = {1}, publisher = {Springer Science and Business Media LLC}, issn = {2055-5008}, doi = {10.1038/s41522-024-00531-7}, pages = {14}, abstract = {Insect gut microbiomes play a crucial role in the insect development and are shaped, among other factors, by the specialized insect diet habits as well as the morphological structure of the gut. Rose chafers (Pachnoda spp.; Coleoptera: Scarabaeidae) have a highly differentiated gut characterized by a pronounced hindgut dilation which resembles a miniaturized rumen. Specifically, the species Pachnoda marginata has not been previously studied in detail in terms of microbial ecology. Here, we show a fine scale study of the highly compartmentalized gut of P. marginata by using amplicon and metagenomic sequencing to shed light on the bacterial, archaeal and fungal communities thriving in each section of the gut. We found a microbial gradient along the gut from aerobic (foregut) to strictly anaerobic communities (hindgut). In addition, we have characterized interesting biological activities and metabolic pathways of gut microbial communities related to cellulose degradation, methane production and sulfate reduction. Taken together, our results reveal the highly diverse microbial community and the potential of P. marginata gut as a source of industrially relevant microbial diversity.}, language = {en} }