@misc{BoeckerZybinNiemaxetal., author = {Boecker, Daniel and Zybin, Alexander and Niemax, Kay and Grunwald, Christian and Mirsky, Vladimir M.}, title = {Noise reduction by multiple referencing in surface plasmon resonance imaging}, series = {Review of Scientific Instruments}, volume = {79}, journal = {Review of Scientific Instruments}, number = {2}, issn = {1527-2400}, doi = {10.1063/1.2888527}, pages = {023110}, abstract = {The analytical performance of surface plasmon resonance imaging with charge coupled device detection can be improved significantly by splitting a macroscopic sensing surface into multiple microscopic neighboring sensing and referencing subareas. It is shown that such a multiple referencing reduces intensity fluctuations across the total sensing area and, therefore, improves the signal/noise (S/N)ratio proportional to the splitting factor. The approach is demonstrated by detection of biotin binding to a monolayer of streptavidin. An effective variation of the reflected intensity of about 10⁻⁴, which corresponds to the refraction index variation of 3 x 10⁻⁶, was detected with a S/N ratio about 10 without any temperature stabilization of the sensing area.}, language = {en} } @misc{ZybinBoeckerMirskyetal., author = {Zybin, Alexander and Boecker, Daniel and Mirsky, Vladimir M. and Niemax, Kay}, title = {Enhancement of the detection power of surface plasmon resonance measurements by optimization of the reflection angle}, series = {Analytical Chemistry}, volume = {79}, journal = {Analytical Chemistry}, number = {11}, issn = {0003-2700}, doi = {10.1021/ac070074u}, pages = {4233 -- 4236}, abstract = {The detection limit of surface plasmon resonance (SPR) measurements has been improved by a factor of approximately 2-3.5 if the angle of incidence was near to the reflection minimum of the SPR resonance curve instead at the position of the steepest slope, the standard alignment in SPR imaging. The enhancement of the detection power, a result of signal-to-noise optimization, is demonstrated by applying a photodiode and a CCD camera for SPR detection. The experimental data are compared with data expected from theory.}, language = {en} }