@misc{KnuetterHiemannBrummaetal., author = {Kn{\"u}tter, Ilka and Hiemann, Rico and Brumma, Therese and B{\"u}ttner, Thomas and Großmann, Kai and Cusini, Marco and Pregnolato, Francesca and Borghi, Maria Orietta and Anderer, Ursula and Conrad, Karsten and Reinhold, Dirk and Roggenbuck, Dirk and Csernok, Elena}, title = {Automated interpretation of ANCA patterns - a new approach in the serology of ANCA-associated vasculitis}, series = {Arthritis Research \& Therapy}, volume = {14}, journal = {Arthritis Research \& Therapy}, issn = {1465-9913}, doi = {10.1186/ar4119}, pages = {10}, language = {en} } @misc{NalliSommaAndreolietal., author = {Nalli, Cecilia and Somma, Valentina and Andreoli, Laura and B{\"u}ttner, Thomas and Schierack, Peter and Mahler, Michael and Roggenbuck, Dirk and Tincani, Angela}, title = {Anti-phospholipid IgG antibodies detected by line immunoassay differentiate patients with anti-phospholipid syndrome and other autoimmune diseases}, series = {Autoimmunity Highlights}, volume = {9}, journal = {Autoimmunity Highlights}, number = {1}, issn = {2038-3274}, doi = {10.1007/s13317-018-0106-0}, pages = {11}, language = {en} } @misc{RoggenbuckBorghiSommaetal., author = {Roggenbuck, Dirk and Borghi, Maria Orietta and Somma, Valentina and B{\"u}ttner, Thomas and Schierack, Peter and Hanack, Katja and Grossi, Claudia and Bodio, Caterina and Macor, Paolo and Landenberg, Philipp von and Boccellato, Francesco and Mahler, Michael and Meroni, Pier Luigi}, title = {Antiphospholipid antibodies detected by line immunoassay differentiate among patients with antiphospholipid syndrome, with infections and asymptomatic carriers}, series = {Arthritis Research \& Therapy}, volume = {18}, journal = {Arthritis Research \& Therapy}, number = {111}, issn = {1465-9913}, doi = {10.1186/s13075-016-1018-x}, pages = {14}, language = {en} } @misc{ThalerBietenbeckSteigerwaldetal., author = {Thaler, Markus A. and Bietenbeck, Andreas and Steigerwald, Udo and B{\"u}ttner, Thomas and Schierack, Peter and Lindhoff-Last, Edelgard and Roggenbuck, Dirk and Luppa, Peter B.}, title = {Evaluation of the sensitivityficity of a novel line immunoassay for the detection of criteria and non-criteria antiphospholipid antibodies in comparison to establishedELISAs}, series = {PLoS One}, volume = {14}, journal = {PLoS One}, number = {7}, issn = {1932-6203}, doi = {10.1371/journal.pone.0220033}, pages = {17}, language = {en} } @misc{EmmeneggerKumarEmmeneggeretal., author = {Emmenegger, Marc and Kumar, Sreedhar Saseendran and Emmenegger, Vishalini and Malinauskas, Tomas and B{\"u}ttner, Thomas and Rose, Laura and Schierack, Peter and Sprinzl, Martin F. and Sommer, Clemens J. and Lackner, Karl J. and Aguzzi, Adriano and Roggenbuck, Dirk and Frauenknecht, Katrin B. M.}, title = {Anti-prothrombin autoantibodies enriched after infection with SARS-CoV-2 and influenced by strength of antibody response against SARS-CoV-2 proteins}, series = {PLoS pathogens}, volume = {17}, journal = {PLoS pathogens}, number = {12}, issn = {1553-7374}, doi = {10.1371/journal.ppat.1010118}, language = {en} } @misc{LyuFuWilczoketal., author = {Lyu, Yu-Xuan and Fu, Qiang and Wilczok, Dominika and Ying, Kejun and King, Aaron and Antebi, Adam and Vojta, Aleksandar and Stolzing, Alexandra and Moskalev, Alexey and Georgievskaya, Anastasia and Maier, Andrea B. and Olsen, Andrea and Groth, Anja and Simon, Anna Katharina and Brunet, Anne and Jamil, Aisyah and Kulaga, Anton and Bhatti, Asif and Yaden, Benjamin and Pedersen, Bente Klarlund and Schumacher, Bj{\"o}rn and Djordjevic, Boris and Kennedy, Brian and Chen, Chieh and Huang, Christine Yuan and Correll, Christoph U. and Murphy, Coleen T. and Ewald, Collin Y. and Chen, Danica and Valenzano, Dario Riccardo and Sołdacki, Dariusz and Erritzoe, David and Meyer, David and Sinclair, David A. and Chini, Eduardo Nunes and Teeling, Emma C. and Morgen, Eric and Verdin, Eric and Vernet, Erik and Pinilla, Estefano and Fang, Evandro F. and Bischof, Evelyne and Mercken, Evi M. and Finger, Fabian and Kuipers, Folkert and Pun, Frank W. and Gy{\"u}lveszi, Gabor and Civiletto, Gabriele and Zmudze, Garri and Blander, Gil and Pincus, Harold A. and McClure, Joshua and Kirkland, James L. and Peyer, James and Justice, Jamie N. and Vijg, Jan and Gruhn, Jennifer R. and McLaughlin, Jerry and Mannick, Joan and Passos, Jo{\~a}o and Baur, Joseph A. and Betts-LaCroix, Joe and Sedivy, John M. and Speakman, John R. and Shlain, Jordan and Maltzahn, Julia von and Andreasson, Katrin I. and Moody, Kelsey and Palikaras, Konstantinos and Fortney, Kristen and Niedernhofer, Laura J. and Rasmussen, Lene Juel and Veenhoff, Liesbeth M. and Melton, Lisa and Ferrucci, Luigi and Quarta, Marco and Koval, Maria and Marinova, Maria and Hamalainen, Mark and Unfried, Maximilian and Ringel, Michael S. and Filipovic, Milos and Topors, Mourad and Mitin, Natalia and Roy, Nawal and Pintar, Nika and Barzilai, Nir and Binetti, Paolo and Singh, Parminder and Kohlhaas, Paul and Robbins, Paul D. and Rubin, Paul and Fedichev, Peter O. and Kamya, Petrina and Mu{\~n}oz-Canoves, Pura and de Cabo, Rafael and Faragher, Richard G. A. and Konrad, Rob and Ripa, Roberto and Mansukhani, Robin and B{\"u}ttner, Sabrina and Wickstr{\"o}m, Sara A. and Brunemeier, Sebastian and Jakimov, Sergey and Luo, Shan and Rosenzweig-Lipson, Sharon and Tsai, Shih-Yin and Dimmeler, Stefanie and Rando, Thomas A. and Peterson, Tim R. and Woods, Tina and Wyss-Coray, Tony and Finkel, Toren and Strauss, Tzipora and Gladyshev, Vadim N. and Longo, Valter D. and Dwaraka, Varun B. and Gorbunova, Vera and Acosta-Rodr{\´i}guez, Victoria A. and Sorrentino, Vincenzo and Sebastiano, Vittorio and Li, Wenbin and Suh, Yousin and Zhavoronkov, Alex and Scheibye-Knudsen, Morten and Bakula, Daniela}, title = {Longevity biotechnology: bridging AI, biomarkers, geroscience and clinical applications for healthy longevity}, series = {Aging}, volume = {16}, journal = {Aging}, number = {20}, publisher = {Impact Journals, LLC}, issn = {1945-4589}, doi = {10.18632/aging.206135}, pages = {12955 -- 12976}, language = {en} } @misc{RoeiBorisYehudaetal., author = {Roei, Tulchinsky and Boris, Gilburd and Yehuda, Shovman and Milena, Tocut and Eleanor, Zeruya and Ariel, Binyaminov and Tima, Davidson and B{\"u}ttner, Thomas and Nasser, Abdullah and Michel, Juliane and R{\"o}diger, Stefan and Schierack, Peter and Howard, Amital and Roggenbuck, Dirk and Yehuda, Shoenfeld and Ora, Shovman}, title = {CytoBead ANA 2 assay : a novel method for the detection of antinuclear antibodies}, series = {Scientific reports}, volume = {15}, journal = {Scientific reports}, number = {1}, publisher = {Nature Publishing Group UK}, address = {London}, issn = {2045-2322}, doi = {10.1038/s41598-025-04583-3}, pages = {1 -- 11}, abstract = {Detection of anti-nuclear autoantibodies (ANA) is based on a two-step algorithm including indirect immunofluorescence (IIF) on HEp2 cells and subsequent reflex/confirmatory testing for specific autoantibodies. Simultaneous cell- and microbead-based autoantibody detection by IIF may be utilized for the evaluation of systemic autoimmune rheumatic diseases (SARDs). In the present study, we assessed the performance of CytoBead ANA 2 in the detection of ANA and ANA-specific autoantibodies, compared to ANA IIF and BioPlex™ 2200. We also tested the ability of CytoBead ANA DFS-70 to identify dense-fine speckled (DFS) pattern associated with anti-DFS70 antibodies in non-SARDs patients. Hundred-twelve routine sera samples were assessed by manual CytoBead ANA 2 for the presence of ANA and specific autoantibodies. In parallel, these samples were analyzed by HEp2 ANA IIF test and a subsequent multiplexed assay BioPlex™ 2200 ANA. Twenty-nine ANA-positive samples obtained from non-SARDs patients and exhibiting DFS pattern by ANA IIF were further tested by CytoBead ANA DFS-70. A substantial agreement was observed between classical ANA IIF and manual CytoBead ANA 2 for the detection of ANA (k = 0.74). Discordant results were mainly associated with the presence of anti-SSA/Ro antibodies detected by CytoBead ANA 2 in ANA IIF negative patients. A good to almost perfect agreement was found between CytoBead ANA 2 and BioPlex™ 2200 for detection of specific antibodies with kappa values ranging from 0.70 to 0.90. Twenty samples (68.9\%) obtained from 29 ANA IIF positive without SARDs patients exhibited DFS pattern in CytoBead ANA DFS-70, which confirmed the presence of anti-DFS70 antibodies. The diagnostic performance of manual CytoBead ANA 2 for ANA screening and detection of ANA specific antibodies is comparable to the diagnostic performance of ANA IIF followed by BioPlex™ 2200. This novel one-step assay enables simultaneous ANA screening and confirmation and represents a promising alternative approach to the time-consuming and costly two-tier ANA analysis.}, language = {en} }