@misc{KotlarskiBlockBoehmetal., author = {Kotlarski, Sven and Block, Alexander and B{\"o}hm, Ursula and Jacob, Daniela and Keuler, Klaus and Knoche, Richard and Rechid, Diana and Walter, Artur}, title = {Regional Climate Model Simulations as Input for Hydrological Applications: Evaluation of Uncertainties}, series = {Advances in Geoscience}, journal = {Advances in Geoscience}, number = {5}, doi = {10.5194/adgeo-5-119-2005}, pages = {119 -- 125}, language = {en} } @techreport{BoehmKueckenAhrensetal., author = {B{\"o}hm, Uwe and K{\"u}cken, Martin and Ahrens, Wilfried and Block, Alexander and Hauffe, Detlef and Keuler, Klaus and Rockel, Burkhardt and Will, Andreas}, title = {CLM - The Climate Version of LM: Brief Description and Long-Term Applications}, publisher = {COSMO Newsletter}, address = {Offenbach, Germany}, pages = {11}, language = {en} } @misc{OctorinaBoehmMartinCreuzburg, author = {Octorina, Pelita and B{\"o}hm, Alexander and Martin-Creuzburg, Dominik}, title = {Morphological defences and defence-cost trade-offs in Daphnia in response to two co-occurring invertebrate predators}, series = {Freshwater Biology}, volume = {67}, journal = {Freshwater Biology}, number = {5}, issn = {1365-2427}, doi = {10.1111/fwb.13888}, pages = {883 -- 892}, language = {en} } @misc{HiemannHilgerMicheletal., author = {Hiemann, Rico and Hilger, Nadja and Michel, J{\"o}rg and Nitschke, J{\"o}rg and B{\"o}hm, Alexander and Anderer, Ursula and Weigert, Martin and Sack, Ulrich}, title = {Automatic Analysis of Immunofluorescence Patterns of HEp-2 Cells}, series = {Annals of the New York Academy of Sciences}, volume = {1109}, journal = {Annals of the New York Academy of Sciences}, number = {1}, issn = {0077-8923}, doi = {10.1196/annals.1398.042}, pages = {358 -- 371}, abstract = {The standard screening test for the recognition of autoimmune diseases is the proof of autoantibodies in serum of patients by indirect immunofluorescence (IIF) based on HEp-2 cells. Manual evaluation of this test is very subjective, slow, and there are no objective parameters as guidelines available. Interlaboratory tests showed occasionally large deviations in the test evaluation resulting in a high variance of results. The aim of this project is fast, objective, safe, and economical automatic analysis of HEp-2 IIF patterns. Images of IIF patterns were completely and automatically captured using an inverse motorized fluorescence microscope. Thereby, device-specific parameters were controlled automatically, too. For fast analysis of IIF patterns new algorithms of image processing were developed. Artifacts were recognized and excluded from analysis by the developed software. Analysis of more than 80,000 images clearly demonstrated full automatization and fast processing of IIF patterns. Additionally serum-specific fluorescence could be easily distinguished from background. Even very weak but positive patterns can be recognized and used for diagnosis. A detailed separation into different basic patterns is possible. Objective, fast, and disease-related economical analysis of HEp-2 immunofluorescence patterns is feasible. The implemented software algorithms allowed a mathematical way of describing IIF patterns and can therefore be a useful tool for the needed standardization process.}, language = {en} } @misc{BoehmKueckenHauffeetal., author = {B{\"o}hm, Uwe and K{\"u}cken, Martin and Hauffe, Detlef and Gerstengarbe, F.-W. and Werner, P. C. and Flechsig, Michael and Keuler, Klaus and Block, Alexander and Ahrens, Wilfried and Nocke, Th.}, title = {Reliability of regional climate model simulations of extremes and of long-term climate}, series = {Natural Hazards and Earth System Sciences}, volume = {4}, journal = {Natural Hazards and Earth System Sciences}, number = {3}, doi = {10.5194/nhess-4-417-2004}, pages = {417 -- 431}, abstract = {We present two case studies that demonstrate how a common evaluation methodology can be used to assess the reliability of regional climate model simulations from different fields of research. In Case I, we focused on the agricultural yield loss risk for maize in Northeastern Brazil during a drought linked to an El-Ni{\~n}o event. In Case II, the present-day regional climatic conditions in Europe for a 10-year period are simulated. To comprehensively evaluate the model results for both kinds of investigations, we developed a general methodology. On its basis, we elaborated and implemented modules to assess the quality of model results using both advanced visualization techniques and statistical algorithms. Besides univariate approaches for individual near-surface parameters, we used multivariate statistics to investigate multiple near-surface parameters of interest together. For the latter case, we defined generalized quality measures to quantify the model's accuracy. Furthermore, we elaborated a diagnosis tool applicable for atmospheric variables to assess the model's accuracy in representing the physical processes above the surface under various aspects. By means of this evaluation approach, it could be demonstrated in Case Study I that the accuracy of the applied regional climate model resides at the same level as that we found for another regional model and a global model. Excessive precipitation during the rainy season in coastal regions could be identified as a major contribution leading to this result. In Case Study II, we also identified the accuracy of the investigated mean characteristics for near-surface temperature and precipitation to be comparable to another regional model. In this case, an artificial modulation of the used initial and boundary data during preprocessing could be identified as the major source of error in the simulation. Altogether, the achieved results for the presented investigations indicate the potential of our methodology to be applied as a common test bed to different fields of research in regional climate modeling.}, language = {en} } @misc{BoehmKueckenAhrensetal., author = {B{\"o}hm, Uwe and K{\"u}cken, Martin and Ahrens, Wilfried and Block, Alexander and Hauffe, Detlef and Keuler, Klaus and Rockel, Burkhardt and Will, Andreas}, title = {The Climate Version of LM: Brief Description and Long-Term Applications}, series = {COSMO Newsletter}, journal = {COSMO Newsletter}, number = {6}, pages = {225 -- 235}, language = {en} } @inproceedings{KolendaBurdukiewiczSzaboetal., author = {Kolenda, Rafał and Burdukiewicz, Michał and Szabo, M. and Orlowska, Aleksandra and Nitschke, J{\"o}rg and B{\"o}hm, Alexander and Schierack, Peter}, title = {Influence of Salmonella FimH sequence variation on binding to pancreatic secretory granule membrane major glycoprotein GP2 of human and porcine origin}, series = {5th ASM Conference on Salmonella, Potsdam, 29.08-01.09.2016}, booktitle = {5th ASM Conference on Salmonella, Potsdam, 29.08-01.09.2016}, publisher = {American Society for Microbiology}, address = {Washington, DC}, pages = {S. 125}, language = {en} } @misc{AwanSchiebelBoehmetal., author = {Awan, Asad Bashir and Schiebel, Juliane and B{\"o}hm, Alexander and Nitschke, J{\"o}rg and Sarwar, Yasra and Schierack, Peter and Ali, Aamir}, title = {Association of biofilm formation and cytotoxic potential with multidrug resistance in clinical isolates of Pseudomonas aeruginosa}, series = {EXCLI journal}, volume = {18}, journal = {EXCLI journal}, issn = {1611-2156}, pages = {79 -- 90}, language = {en} } @misc{FroemmelLehmannRoedigeretal., author = {Fr{\"o}mmel, Ulrike and Lehmann, Werner and R{\"o}diger, Stefan and B{\"o}hm, Alexander and Nitschke, J{\"o}rg and Weinreich, J{\"o}rg and Groß, Julia and Roggenbuck, Dirk and Zinke, Olaf and Ansorge, Hermann and Vogel, Steffen and Klemm, Per and Wex, Thomas and Schr{\"o}der, Christian and Wieler, Lothar H. and Schierack, Peter}, title = {Adhesion of human and animal Escherichia coli strains in association with their virulence-associated genes and phylogenetic origins}, series = {Applied and environmental microbiology}, volume = {79}, journal = {Applied and environmental microbiology}, number = {19}, issn = {1098-5336}, doi = {10.1128/AEM.01384-13}, pages = {5814 -- 5829}, abstract = {Intestinal colonization is influenced by the ability of the bacterium to inhabit a niche, which is based on the expression of colonization factors. Escherichia coli carries a broad range of virulence-associated genes (VAGs) which contribute to intestinal (inVAGs) and extraintestinal (exVAGs) infection. Moreover, initial evidence indicates that inVAGs and exVAGs support intestinal colonization. We developed new screening tools to genotypically and phenotypically characterize E. coli isolates originating in humans, domestic pigs, and 17 wild mammal and avian species. We analyzed 317 isolates for the occurrence of 44 VAGs using a novel multiplex PCR microbead assay (MPMA) and for adhesion to four epithelial cell lines using a new adhesion assay. We correlated data for the definition of new adhesion genes. inVAGs were identified only sporadically, particularly in roe deer (Capreolus capreolus) and the European hedgehog ( Erinaceus europaeus). The prevalence of exVAGs depended on isolation from a specific host. Human uropathogenic E. coli isolates carried exVAGs with the highest prevalence, followed by badger (Meles meles) and roe deer isolates. Adhesion was found to be very diverse. Adhesion was specific to cells, host, and tissue, though it was also unspecific. Occurrence of the following VAGs was associated with a higher rate of adhesion to one or more cell lines: afa-dra, daaD, tsh, vat, ibeA, fyuA, mat, sfa-foc, malX, pic, irp2, and papC. In summary, we established new screening methods which enabled us to characterize large numbers of E. coli isolates. We defined reservoirs for potential pathogenic E. coli. We also identified a very broad range of colonization strategies and defined potential new adhesion genes.}, language = {en} } @misc{RoedigerLehmannFroemmeletal., author = {R{\"o}diger, Stefan and Lehmann, Werner and Fr{\"o}mmel, Ulrike and B{\"o}hm, Alexander and Nitschke, J{\"o}rg and Burdukiewicz, Michał and Schr{\"o}der, Christian and Dangla, R{\´e}mi and Droniou, Magali and Schierack, Peter}, title = {The Modi Operandi of the VideoScan Platform for the Detection and Analysis of Nucleic Acids. Life Science Day 2013 \& 17. Leibniz Conference of Advanced Science, Freie Universit{\"a}t Berlin, 24.10.2013}, pages = {1}, language = {en} } @misc{LiebschRoedigerBoehmetal., author = {Liebsch, Claudia and R{\"o}diger, Stefan and B{\"o}hm, Alexander and Nitschke, J{\"o}rg and Weinreich, J{\"o}rg and Fruth, Angelika and Roggenbuck, Dirk and Lehmann, Werner and Schedler, Uwe and Juretzek, Thomas and Schierack, Peter}, title = {Solid-phase microbead array for multiplex O-serotyping of Escherichia coli}, series = {Microchimica Acta}, volume = {184}, journal = {Microchimica Acta}, number = {5}, issn = {0026-3672}, doi = {10.1007/s00604-017-2088-4}, pages = {1405 -- 1415}, language = {en} } @inproceedings{RoedigerBoehmNitschkeetal., author = {R{\"o}diger, Stefan and B{\"o}hm, Alexander and Nitschke, J{\"o}rg and Stavitskaya, Luba and Gruner, Melanie and Kundzer, Alena V. and Volkova, Margarita V. and Generalov, I. and Schmidt, Carsten and Schr{\"o}der, Christian and Roggenbuck, Dirk and Schierack, Peter}, title = {Development of a Method for Multiplex Real-Time Analysis of Enzymatic Activity on a Microbead-Chip}, series = {Infection, tumors and autoimmunity, report on the 11th Dresden Symposium on Autoantibodies held in Dresden on September 1-4, 2013}, booktitle = {Infection, tumors and autoimmunity, report on the 11th Dresden Symposium on Autoantibodies held in Dresden on September 1-4, 2013}, publisher = {Pabst Science Publ.}, address = {Lengerich [u.a.]}, isbn = {978-3-89967-881-9}, pages = {275 -- 280}, language = {en} } @inproceedings{KolendaBurdukiewiczSchiebeletal., author = {Kolenda, Rafał and Burdukiewicz, Michał and Schiebel, Juliane and Szabo, Istvan and Orlowska, Aleksandra and Nitschke, J{\"o}rg and B{\"o}hm, Alexander and Schierack, Peter}, title = {Adhesion of Salmonella to pancreatic secretory granule membrane major glycoprotein GP2 depends on FimH sequence variation}, series = {Abstractbook, Microbiology and Infection, 5th Joint Conference of the DGHM \& VAAM, VAAM Annual Meeting 2017, 69th Annual Meeting of the DGHM}, booktitle = {Abstractbook, Microbiology and Infection, 5th Joint Conference of the DGHM \& VAAM, VAAM Annual Meeting 2017, 69th Annual Meeting of the DGHM}, publisher = {Spektrumverlag}, address = {Heidelberg}, pages = {S. 118}, language = {en} } @misc{KolendaBurdukiewiczSchiebeletal., author = {Kolenda, Rafał and Burdukiewicz, Michał and Schiebel, Juliane and R{\"o}diger, Stefan and Sauer, Lysann and Szabo, Istvan and Orlowska, Aleksandra and Weinreich, J{\"o}rg and Nitschke, J{\"o}rg and B{\"o}hm, Alexander and Gerber, Ulrike and Roggenbuck, Dirk and Schierack, Peter}, title = {Adhesion of Salmonella to Pancreatic Secretory Granule Membrane Major Glycoprotein GP2 of Human and Porcine Origin Depends on FimH Sequence Variation}, series = {Frontiers in Microbiology}, volume = {9}, journal = {Frontiers in Microbiology}, number = {1905}, issn = {1664-302X}, doi = {10.3389/fmicb.2018.01905}, pages = {15}, language = {en} } @inproceedings{BurdukiewiczMikolajczykSzaboetal., author = {Burdukiewicz, Michał and Mikolajczyk, A. and Szabo, Istvan and Nitschke, J{\"o}rg and B{\"o}hm, Alexander and Schierack, Peter and Kolenda, Rafał}, title = {Automated enumeration of Salmonella spp. in cell line infection assays and screening of cell line infectivity among various Salmonella isolates}, series = {5th ASM Conference on Salmonella, Potsdam, 29.08-01.09.2016}, booktitle = {5th ASM Conference on Salmonella, Potsdam, 29.08-01.09.2016}, publisher = {American Society for Microbiology}, address = {Washington, DC}, pages = {S. 127}, language = {en} } @incollection{RoedigerSchierackBoehmetal., author = {R{\"o}diger, Stefan and Schierack, Peter and B{\"o}hm, Alexander and Nitschke, J{\"o}rg and Berger, Ingo and Fr{\"o}mmel, Ulrike and Schmidt, Carsten and Ruhland, Mirko and Schimke, Ingolf and Roggenbuck, Dirk and Lehmann, Werner and Schr{\"o}der, Christian}, title = {A highly versatile microscope imaging technology platform for the multiplex real-time detection of biomolecules and autoimmune antibodies}, series = {Molecular diagnostics}, booktitle = {Molecular diagnostics}, editor = {Seitz, Harald and Berger, Ingo}, publisher = {Springer}, address = {Berlin [u.a.]}, isbn = {978-3-642-37690-0}, pages = {35 -- 74}, language = {en} } @misc{SchiebelNoackRoedigeretal., author = {Schiebel, Juliane and Noack, Jonas and R{\"o}diger, Stefan and Kammel, Anne and Menzel, Friederike and Schwibbert, Karin and Weise, Matthias and Weiss, Romano and B{\"o}hm, Alexander and Nitschke, J{\"o}rg and Elimport, Alexey and Roggenbuck, Dirk and Schierack, Peter}, title = {Analysis of three-dimensional biofilms on different material surfaces}, series = {Biomaterials Science}, volume = {8}, journal = {Biomaterials Science}, number = {12}, issn = {2047-4849}, doi = {10.1039/D0BM00455C}, pages = {3500 -- 3510}, language = {en} } @misc{SchiebelBoehmNitschkeetal., author = {Schiebel, Juliane and B{\"o}hm, Alexander and Nitschke, J{\"o}rg and Burdukiewicz, Michał and Weinreich, J{\"o}rg and Ali, Aamir and Roggenbuck, Dirk and R{\"o}diger, Stefan and Schierack, Peter}, title = {Genotypic and phenotypic characteristics in association with biofilm formation in different pathotypes of human clinical Escherichia coli isolates}, series = {Applied and Environmental Microbiology}, volume = {83}, journal = {Applied and Environmental Microbiology}, number = {24}, issn = {1098-5336}, doi = {10.1128/AEM.01660-17}, language = {en} } @misc{RoedigerRuhlandSchroederetal., author = {R{\"o}diger, Stefan and Ruhland, Mirko and Schr{\"o}der, Christian and Großmann, Kai and B{\"o}hm, Alexander and Nitschke, J{\"o}rg and Berger, Ingo and Schimke, Ingolf and Schierack, Peter}, title = {Fluorescence dye adsorption assay to quantify carboxyl groups on the surface of poly(methyl methacrylate) microbeads}, series = {Analytical Chemistry}, volume = {83}, journal = {Analytical Chemistry}, number = {9}, issn = {0003-2700}, doi = {10.1021/ac103277s}, pages = {3379 -- 3385}, language = {en} } @misc{FroemmelBoehmNitschkeetal., author = {Fr{\"o}mmel, Ulrike and B{\"o}hm, Alexander and Nitschke, J{\"o}rg and Weinreich, J{\"o}rg and Groß, Julia and R{\"o}diger, Stefan and Wex, Thomas and Ansorge, Hermann and Zinke, Olaf and Schr{\"o}der, Christian and Roggenbuck, Dirk and Schierack, Peter}, title = {Adhesion patterns of commensal and pathogenic Escherichia coli from humans and wild animals on human and porcine epithelial cell lines}, series = {Gut Pathogens}, volume = {5}, journal = {Gut Pathogens}, number = {31}, issn = {1757-4749}, doi = {10.1186/1757-4749-5-31}, pages = {8}, abstract = {Abstract BACKGROUND: Different strategies of colonization or infection by E. coli result in formation of certain adhesion patterns which help also in classifying intestinal E. coli into pathotypes. Little is known about adhesion patterns and host- and tissue adaption of commensal E. coli and about E. coli originating in clinically healthy hosts carrying pathotype-specific virulence-associated genes. FINDINGS: Adhesion pattern of E. coli (n = 282) from humans and from 18 animal species were verified on intestinal human Caco-2 and porcine IPEC-J2 cells and, furthermore, for comparison on human urinary bladder 5637, porcine kidney PK-15 epithelial and HEp-2 cells. The analysis was carried out on 150,000 images of adhesion assays.Adhesion patterns were very diverse; 88 isolates were completely non-adherent, whereas 194 adhered to at least one cell line with the dominant adhesion patterns "diffusely distributed" and "microcolony formation". Adhesion patterns "chains" and "clumps" were also visible. Chain formation was mediated by the presence of epithelial cells. Clump formation was very specific on only the 5637 cell line. All enteropathogenic (eae+) E. coli (EPEC; n = 14) were able to form microcolonies which was cell line specific for each isolate. Most EPEC formed microcolonies on intestinal IPEC-J2 and Caco-2 but several also on urinary tract cells. Shigatoxin-producing (stx+) E. coli (n = 10) showed no specific adhesion patterns. CONCLUSIONS: E. coli isolates were highly diverse. Commensal and pathogenic isolates can adhere in various forms, including diffuse distribution, microcolonies, chains and clumps. Microcolony formation seems to be a global adhesion strategy also for commensal E. coli.}, language = {en} } @inproceedings{RoedigerBoehmNitschkeetal., author = {R{\"o}diger, Stefan and B{\"o}hm, Alexander and Nitschke, J{\"o}rg and Stavitskaya, Luba and Gruner, Melanie and Kundzer, Alena V. and Volkova, Margarita V. and Generalov, I. and Schmidt, Carsten and Schr{\"o}der, Christian and Roggenbuck, Dirk and Schierack, Peter}, title = {Development of a Method for Multiplex Real-Time Analysis of Enzymati c Activity on a Microbead-Chip.}, series = {Infections, Tumors and Autoimmunity, Report on the 11th Dresden Symposium on Autoantibodies held in Dresden on September 01. - 04., 2013}, booktitle = {Infections, Tumors and Autoimmunity, Report on the 11th Dresden Symposium on Autoantibodies held in Dresden on September 01. - 04., 2013}, publisher = {Pabst Science Publ.}, address = {Lengerich}, isbn = {978-3-89967-881-9}, pages = {280}, language = {en} }