@misc{LukosiusLippertDabrowskietal., author = {Lukosius, Mindaugas and Lippert, Gunther and Dabrowski, Jarek Marek and Kitzmann, Julia and Lisker, Marco and Kulse, Philipp and Kr{\"u}ger, Andreas and Fursenko, Oksana and Costina, Ioan and Trusch, Andreas and Yamamoto, Yuji and Wolff, Andre and Mai, Andreas and Schr{\"o}der, Thomas}, title = {Graphene Synthesis and Processing on Ge Substrates}, series = {ECS transactions}, volume = {75}, journal = {ECS transactions}, number = {8}, issn = {1938-6737}, doi = {10.1149/07508.0533ecst}, pages = {533 -- 540}, language = {en} } @misc{MaussNakovWenzeletal., author = {Mauß, Fabian and Nakov, Galin and Wenzel, Paul and Steiner, R{\"u}diger and Kr{\"u}ger, Christian and Zhang, Yongzeh and Rawat, Rajesh and Borg, Andreas and Perlman, Cathleen and Fr{\"o}jd, Karin and Lehtiniemi, Harry}, title = {Soot Simulation under Diesel Engine Conditions Using a Flamelet Approach}, series = {SAE International Journal of Engines}, volume = {2}, journal = {SAE International Journal of Engines}, number = {2}, issn = {1946-3936}, pages = {89 -- 104}, language = {en} } @incollection{UlrichSchlegelMertensetal., author = {Ulrich, Rainer G. and Schlegel, Matthias and Mertens, Marc and Groschup, Martin H. and Schmidt-Chanasit, Jonas and Plenge-B{\"o}nig, Anita and Jacob, Jens and Pelz, Hans-Joachim and Freise, Jona and Wenk, Matthias and Thiel, J{\"o}rg and Triebenbacher, Cornelia and Schmolz, Eric and Kurth, Andreas and Kr{\"u}ger, Frank and R{\"u}he, Ferdinand and Kiffner, Christian and Ansorge, Hermann and Gerwin, Werner and Wegener, Wolfgang and M{\"u}ller, J{\"o}rg and Bemmann, Margit and Wolf, Ronny and Otto, Lutz-Florian and Oehme, Rainer and Pfeffer, Martin and Heckel, Gerald and Schex, Susanne and Essbauer, Sandra S.}, title = {Netzwerk Nagetier-{\"u}bertragene Pathogene: Monitoring von Hantavirus-Infektionen in Deutschland}, series = {Wildhygiene, Wildtierkrankheiten, Parasiten, Epidemiologie}, booktitle = {Wildhygiene, Wildtierkrankheiten, Parasiten, Epidemiologie}, publisher = {Ges. f{\"u}r Wildtier- und Jagdforschung}, address = {Halle/Saale}, isbn = {978-378-88131-2-3}, issn = {1436-3895}, pages = {229 -- 250}, language = {de} } @misc{LauRangarajanKruegerGengeetal., author = {Lau, S. and Rangarajan, R. and Kr{\"u}ger-Genge, Anne and Braune, Steffen and K{\"u}pper, Jan-Heiner and Lendlein, Andreas and Jung, Friedrich}, title = {Age-related morphology and function of human arterial endothelial cells}, series = {Clinical Hemorheology and Microcirculation}, volume = {74}, journal = {Clinical Hemorheology and Microcirculation}, number = {1}, issn = {1875-8622}, doi = {10.3233/CH-199238}, pages = {93 -- 107}, abstract = {Endothelialization of cardiovascular implants is regarded as a promising strategy for long-term compatibility. While umbilical vein endothelial cells are typically applied in research, human arterial endothelial cells (HAEC) from elderly donors would be the obvious source for autologous cellularization strategies. In our approach, HAEC from 16 donors of varying age (16-63 years) were divided into two groups (<30 years and >30 years) and analyzed regarding morphology, viability, proliferation, function and senescence status. No age-related differences were found regarding morphology, viability, density, prostacyclin and nitrite secretion or collagen and laminin production. However, the metabolic activity was slightly decreased (p = 0.0374) and the membrane integrity marginally impaired (p = 0.0404) in cells from older donors. Two out of three senescence assays detected more senescence markers in cells from older donors. According to the assays applied here, HAEC from young and elderly donors up to the age of 63 years could be judged equally suitable for autologous cellularization strategies. However, this finding should be regarded with caution due to the extremely large variability between individual donors. Further studies comprising a larger sample size are necessary to investigate this issue more thoroughly.}, language = {en} } @misc{SchulzKruegerGengeLendleinetal., author = {Schulz, Christian and Kr{\"u}ger-Genge, Anne and Lendlein, Andreas and K{\"u}pper, Jan-Heiner and Jung, Friedrich}, title = {Potential Effects of Nonadherent on Adherent Human Umbilical Venous Endothelial Cells in Cell Culture}, series = {International Journal of Molecular Science}, volume = {22}, journal = {International Journal of Molecular Science}, number = {3}, doi = {10.3390/ijms22031493}, pages = {15}, abstract = {The adherence and shear-resistance of human umbilical venous endothelial cells (HUVEC) on polymers is determined in vitro in order to qualify cardiovascular implant materials. In these tests, variable fractions of HUVEC do not adhere to the material but remain suspended in the culture medium. Nonadherent HUVEC usually stop growing, rapidly lose their viability and can release mediators able to influence the growth and function of the adherent HUVEC. The aim of this study was the investigation of the time dependent behaviour of HUVEC under controlled nonadherent conditions, in order to gain insights into potential influences of these cells on their surrounding environment in particular adherent HUVEC in the context of in vitro biofunctionality assessment of cardiovascular implant materials. Data from adherent or nonadherent HUVEC growing on polystyrene-based cell adhesive tissue culture plates (TCP) or nonadhesive low attachment plates (LAP) allow to calculate the number of mediators released into the culture medium either from adherent or nonadherent cells. Thus, the source of the inflammatory mediators can be identified. For nonadherent HUVEC, a time-dependent aggregation without further proliferation was observed. The rate of apoptotic/dead HUVEC progressively increased over 90\% within two days. Concomitant with distinct blebbing and loss of membrane integrity over time, augmented releases of prostacyclin (PGI2, up to 2.91 ± 0.62 fg/cell) and platelet-derived growth factor BB (PDGF-BB, up to 1.46 ± 0.42 fg/cell) were detected. The study revealed that nonadherent, dying HUVEC released mediators, which can influence the surrounding microenvironment and thereby the results of in vitro biofunctionality assessment of cardiovascular implant materials. Neglecting nonadherent HUVEC bears the risk for under- or overestimation of the materials endothelialization potential, which could lead to the loss of relevant candidates or to uncertainty with regard to their suitability for cardiac applications. One approach to minimize the influence from nonadherent endothelial cells could be their removal shortly after observing initial cell adhesion. However, this would require an individual adaptation of the study design, depending on the properties of the biomaterial used.}, language = {en} } @misc{KruegerGengeSteinbrechtKuepperetal., author = {Kr{\"u}ger-Genge, Anne and Steinbrecht, Susanne and K{\"u}pper, Jan-Heiner and Lendlein, Andreas and Jung, Friedrich}, title = {Evidence for cytostatic effect of cyclophosphamide on human vein endothelial cells in cancer therapy: preliminary in vitro results.}, series = {Clinical Hemorheology and Microcirculation}, volume = {69}, journal = {Clinical Hemorheology and Microcirculation}, number = {1-2}, issn = {1875-8622}, doi = {10.3233/CH-189125}, pages = {267 -- 276}, language = {en} } @misc{KruegerGengeBrauneWalteretal., author = {Kr{\"u}ger-Genge, Anne and Braune, Steffen and Walter, Maria and Kratz, Karl and K{\"u}pper, Jan-Heiner and Krengel, M. and Lendlein, Andreas and Jung, Friedrich}, title = {Influence of surface treatments of poly(n-butyl acrylate) networks on fibroblasts adhesion, morphology and viability}, series = {Clinical Hemorheology and Microcirculation}, volume = {69}, journal = {Clinical Hemorheology and Microcirculation}, number = {1-2}, issn = {1875-8622}, doi = {10.3233/CH-189130}, pages = {305 -- 316}, language = {en} } @misc{KruegerAldarawishKirstenetal., author = {Kr{\"u}ger, David and Aldarawish, Nazeeh and Kirsten, Andreas and Winkelmann, Ralf}, title = {Spritzen von Metall-Hartstoff-Verbundschichten}, series = {Tagungsband zum 21. Werkstofftechnischen Kolloquium, 06. und 07.03.2019 in Chemnitz}, journal = {Tagungsband zum 21. Werkstofftechnischen Kolloquium, 06. und 07.03.2019 in Chemnitz}, editor = {Lampke, Thomas and Wagner, Guntram and Wagner, Martin Franz-Xaver}, publisher = {TU Chemnitz, Fakult{\"a}t f{\"u}r Maschinenbau, Eigenverlag}, address = {Chemnitz}, isbn = {978-3-00-062158-1}, issn = {1439-1597}, pages = {235 -- 243}, abstract = {Die technischen M{\"o}glichkeiten der Herstellung von Metall-Hartstoff-Verbundschichten mit den Verfahren des thermischen Spritzens sind hinsichtlich der Hartstoffkorngr{\"o}ße begrenzt. Diese Einschr{\"a}nkung bedeutet eine geringe Verschleißbest{\"a}ndigkeit der konventionell hergestellten Schichten bei grob-abrasiver Beanspruchung. Untersuchungen mit dem Ziel gr{\"o}bere Hartstoffe in Spritzschichten zu positionieren, waren bisher wenig erfolgreich. Es kommt zu einer Zerst{\"o}rung der separat zugef{\"u}hrten Hartstoffe und einer meist sehr deutlichen Reduzierung der Auftragsrate. Eine Analyse der vorhandenen {\"U}berlegungen und Untersuchungsergebnisse f{\"u}hrte zu der Erkenntnis, dass eine die Hartstoffe und die Matrix anziehende Energiewirkung notwendig ist, um den Verbund zu erzielen. Um eine anziehende Komponente zu erzielen, wurde bei den durchgef{\"u}hrten Untersuchungen eine elektrostatische Aufladung der Hartstoffe benutzt. In den hergestellten Beschichtungen konnten dadurch circa 20 Vol.-\% separat zugef{\"u}hrte Hartstoffe unaufgeschmolzen registriert werden. Eine Beeintr{\"a}chtigung der Auftragsrate war nicht zu registrieren. Werden die Parameter Druck, Temperatur und das chemische Potenzial optimiert, ist auch eine sichere stoffschl{\"u}ssige Einbindung keramischer Hartstoffe in einer Eisenbasis-Matrix nachweisbar. Die Alternative der Integration der Hartstoffe w{\"a}re eine mechanische Verklammerung. Daraus resultierende Risse um den Hartstoff wurden nicht nachgewiesen. Der Hartstoff wirkt in den hergestellten Beschichtungen eindeutig als Keim und nicht als fremdes Element bei der Entstehung des Gef{\"u}ges. Die Ergebnisse der Verschleißversuche an derart hergestellten Beschichtungen verdeutlichen eine nahezu Halbierung der Verschleißrate gegen{\"u}ber der rein metallischen Beschichtung infolge der zugef{\"u}hrten Hartstoffe und ihrer metallischen Einbindung in die Schicht.}, language = {de} } @misc{SchulzKruegerGengeJungetal., author = {Schulz, Christian and Kr{\"u}ger-Genge, Anne and Jung, Friedrich and Lendlein, Andreas}, title = {Aptamer supported in vitro endothelialization of poly(ether imide) films}, series = {Clinical Hemorheology and Microcirculation}, volume = {75}, journal = {Clinical Hemorheology and Microcirculation}, number = {2}, issn = {1875-8622}, doi = {10.3233/CH-190775}, pages = {201 -- 217}, abstract = {Implantation of synthetic small-diameter vascular bypass grafts is often associated with an increased risk of failure, due to thrombotic events or late intimal hyperplasia. As one of the causes an insufficient hemocompatibility of the artificial surface is discussed. Endothelialization of synthetic grafts is reported to be a promising strategy for creating a self-renewing and regulative anti-thrombotic graft surface. However, the establishment of a shear resistant cell monolayer is still challenging. In our study, cyto- and immuno-compatible poly(ether imide) (PEI) films were explored as potential biomaterial for cardiovascular applications. Recently, we reported that the initial adherence of primary human umbilical vein endothelial cells (HUVEC) was delayed on PEI-films and about 9 days were needed to establish a confluent and almost shear resistant HUVEC monolayer. To accelerate the initial adherence of HUVEC, the PEI-film surface was functionalized with an aptamer-cRGD peptide based endothelialization supporting system. With this functionalization the initial adherence as well as the shear resistance of HUVEC on PEI-films was considerable improved compared to the unmodified polymer surface. The in vitro results confirm the general applicability of aptamers for an efficient functionalization of substrate surfaces.}, language = {en} } @misc{LauRangarajanKruegerGengeetal., author = {Lau, S. and Rangarajan, R. and Kr{\"u}ger-Genge, Anne and Braune, Steffen and Kammerer, Sarah and K{\"u}pper, Jan-Heiner and Lendlein, Andreas and Jung, Friedrich}, title = {Effects of acrolein in comparison to its prodrug cyclophosphamide on human primary endothelial cells in vitro}, series = {Toxicology in Vitro}, volume = {62}, journal = {Toxicology in Vitro}, issn = {0887-2333}, doi = {10.1016/j.tiv.2019.104685}, pages = {8}, abstract = {Cyclophosphamide (CPA) is one of the most successful anticancer prodrugs that becomes effective after biotransformation in the liver resulting in the toxic metabolite acrolein. Cancer is often accompanied by thromboembolic events, which might be a result of dysfunctional endothelial cells due to CPA treatment. Here, the effect of 1 mM CPA or acrolein (10/50/100/500 μM) on human umbilical vein endothelial cells (HUVECs) was analyzed after two days of treatment. The addition of CPA or 10 μM acrolein did not affect HUVECs. However, concentrations of 100 μM and 500 μM acrolein significantly reduced the number of adherent cells by 86 ± 13\% and 99 ± 1\% and cell viability by 51 ± 29\% and 93 ± 8\% compared to the control. Moreover, pronounced stress fibers as well as multiple nuclei were observed and von Willebrand factor (vWF) was completely released. Lactate dehydrogenase was 8.5 ± 7.0-fold and 252.9 ± 42.9-fold increased showing a loss of cell membrane integrity. The prostacyclin and thromboxane secretion was significantly increased by the addition of 500 μM acrolein (43.1 ± 17.6-fold and 246.4 ± 106.3-fold) indicating cell activation/pertubation. High doses of acrolein led to HUVEC death and loss of vWF production. This effect might be associated with the increased incidence of thromboembolic events in cancer patients treated with high doses of CPA.}, language = {en} }