TY - GEN A1 - Wang, Mengyi A1 - Wamp, Sabrina A1 - Gibhardt, Johannes A1 - Holland, Gudrun A1 - Schwedt, Inge A1 - Schmidtke, Kai-Uwe A1 - Scheibner, Katrin A1 - Halbedel, Sven A1 - Commichau, Fabian M. T1 - Adaptation of Listeria monocytogenes to perturbation of c-di-AMP metabolism underpins its role in osmoadaptation and identifies a fosfomycin uptake system T2 - Environmental microbiology N2 - The human pathogen Listeria monocytogenes synthesizes and degrades c-di-AMP using the diadenylate cyclase CdaA and the phosphodiesterases PdeA and PgpH respectively. c-di-AMP is essential because it prevents the uncontrolled uptake of osmolytes. Here, we studied the phenotypes of cdaA, pdeA, pgpH and pdeA pgpH mutants with defects in c-di-AMP metabolism and characterized suppressor mutants restoring their growth defects. The characterization of the pdeA pgpH mutant revealed that the bacteria show growth defects in defined medium, a phenotype that is invariably suppressed by mutations in cdaA. The previously reported growth defect of the cdaA mutant in rich medium is suppressed by mutations that osmotically stabilize the c-di-AMP-free strain. We also found that the cdaA mutant has an increased sensitivity against isoleucine. The isoleucine-dependent growth inhibition of the cdaA mutant is suppressed by codY mutations that likely reduce the DNA-binding activity of encoded CodY variants. Moreover, the characterization of the cdaA suppressor mutants revealed that the Opp oligopeptide transport system is involved in the uptake of the antibiotic fosfomycin. In conclusion, the suppressor analysis corroborates a key function of c-di-AMP in controlling osmolyte homeostasis in L. monocytogenes. KW - Listeria monocytogenes KW - c-di-AMP metabolism Y1 - 2022 UR - https://opus4.kobv.de/opus4-UBICO/frontdoor/index/index/docId/29243 UR - https://sfamjournals.onlinelibrary.wiley.com/doi/10.1111/1462-2920.16084?af=R SN - 1462-2920 VL - 24 IS - 9 SP - 4466 EP - 4488 ER -