TY - GEN A1 - Künzel, Stephan R. A1 - Rausch, Johanna S. E. A1 - Schäffer, Charlotte A1 - Hoffmann, Maximilian A1 - Künzel, Karolina A1 - Klapproth, Erik A1 - Kant, Theresa A1 - Herzog, Natalie A1 - Küpper, Jan-Heiner A1 - Lorenz, Kristina A1 - Dudek, Svenja A1 - Emig, Ramona A1 - Ravens, Ursula A1 - Rog‐Zielinska, Eva A. A1 - Peyronnet, Rémi A1 - El‐Armouche, Ali T1 - Modeling atrial fibrosis in vitro - Generation and characterization of a novel human atrial fibroblast cell line T2 - FEBS Open Bio N2 - Atrial fibrillation (AF) is regularly accompanied by cardiac fibrosis and concomitant heart failure. Due to the heterogeneous nature and complexity of fibrosis, the knowledge about the underlying mechanisms is limited, which prevents effective pharmacotherapy. A deeper understanding of cardiac fibroblasts is essential to meet this need. We previously described phenotypic and functional differences between atrial fibroblasts from patients in sinus rhythm and with AF. Herein, we established and characterized a novel human atrial fibroblast line, which displays typical fibroblast morphology and function comparable to primary cells but with improved proliferation capacity and low spontaneous myofibroblast differentiation. These traits make our model suitable for the study of fibrosis mechanisms and for drug screening aimed at developing effective antifibrotic pharmacotherapy. KW - cardiovascular disease KW - cell culture KW - cell lines KW - fibroblasts KW - fibrosis KW - heart Y1 - 2020 UR - https://opus4.kobv.de/opus4-UBICO/frontdoor/index/index/docId/26338 SN - 2211-5463 VL - 10 IS - 7 SP - 1210 EP - 1218 ER -