TY - GEN A1 - Friedrich, Ines A1 - Klassen, Anna A1 - Neubauer, Hannes A1 - Schneider, Dominik A1 - Hertel, Robert A1 - Daniel, Rolf T1 - Living in a Puddle of Mud: Isolation and Characterization of Two Novel Caulobacteraceae Strains Brevundimonas pondensis sp. nov. and Brevundimonas goettingensis sp. nov. T2 - Applied Microbiology N2 - Brevundimonas is a genus of freshwater bacteria belonging to the family Caulobacteraceae. The present study describes two novel species of the genus Brevundimonas (LVF1 T and LVF2 T ). Both were genomically, morphologically, and physiologically characterized. Average nucleotide identity analysis revealed both are unique among known Brevundimonas strains. In silico and additional ProphageSeq analyses resulted in two prophages in the LVF1 T genome and a remnant prophage in the LVF2 T genome. Bacterial LVF1 T cells form an elliptical morphotype, in average 1 µ m in length and 0.46 µ m in width, with a single flagellum. LVF2 T revealed motile cells approximately 1.6 µ m in length and 0.6 µ m in width with a single flagellum, and sessile cell types 1.3 µ m in length and 0.6 µ m in width. Both are Gram-negative, aerobic, have optimal growth at 30 ◦ C (up to 0.5 to 1% NaCl). Both are resistant towards erythromycin, meropenem, streptomycin, tetracycline and vancomycin. Anaerobic growth was observed after 14 days for LVF1 T only. For LVF1 T the name Brevundimonas pondensis sp. nov. and for LVF2 T the name Brevundimonas goettingensis sp. nov. are proposed. Type strains are LVF1 T (=DSM 112304 T = CCUG 74982 T = LMG 32096 T ) and LVF2 T (=DSM 112305 T = CCUG 74983 T = LMG 32097 T ). Y1 - 2021 UR - https://opus4.kobv.de/opus4-UBICO/frontdoor/index/index/docId/27594 UR - https://www.mdpi.com/2673-8007/1/1/5 SN - 2673-8007 VL - 1 IS - 1 SP - 38 EP - 59 ER -