TY - GEN A1 - Hammami, Asma A1 - Raouafi, Noureddine A1 - Mirsky, Vladimir M. T1 - Electrically controlled Michael addition: Addressing of covalent immobilization of biological receptors T2 - Biosensors and Bioelectronics N2 - Electrically addressed covalent immobilization of biomolecules to the defined electrodes of an electrode array is described. It is based on Michael addition of the thiol group of biomolecules to α,β-unsaturated carbonyl groups of benzoquinone. This “click” reaction was tested by immobilization of a number of thiolated compounds on the simplest array consisting of two gold electrodes coated by a self-assembled monolayer of benzoquinone-terminated hexanethiol. Electrically controlled binding of hexanethiol, ferrocenylhexanethiol, human serum albumin and thiol-terminated single-stranded DNA (ssDNA) was investigated. The binding was studied using cyclic voltammetry, X-ray photoelectron spectroscopy and surface plasmon resonance. The reaction requires the oxidized state of the benzoquinone moiety; this can be reached by applying of a moderate anodic potential to the electrode. Surface plasmon resonance measurements demonstrated that the thiol-modified ssDNA immobilized by this technique binds complementary synthetic oligonucleotides or PCR-amplified DNA fragments. The developed technology of electrical addressing of covalent immobilization can be applied for fabrication of sensor arrays. KW - Self-assembled monolayer Immobilization of biomolecules Addressable immobilization Michael addition Cyclic voltammetry Surface plasmon resonance Y1 - 2018 UR - https://opus4.kobv.de/opus4-UBICO/frontdoor/index/index/docId/22849 SN - 1873-4235 SN - 0956-5663 VL - 121 SP - 72 EP - 79 ER -