Induction and Coexpression of Latent Transforming Growth Factor β-Binding Protein-1 and Fibrillin-1 in Experimental Glomerulonephritis

Language
en
Document Type
Article
Issue Date
2013-03-14
Issue Year
2005
Authors
Porst, Markus
Daniel, Christoph
Plank, Christian
Schocklmann, Harald O.
Reinhardt, Dieter P.
Hartner, Andrea
Editor
Abstract

Background: Latent transforming growth factor-β-binding protein 1 (LTBP-1) and fibrillin-1 were shown to colocalize and interact in the extracellular matrix of the skin and vasculature. This interaction may regulate transforming growth factor-β (TGF-β ) activity. TGF-β is an important progression factor for glomerular diseases. We hypothesized that LTBP-1 and fi brillin-1 are coexpressed in the glomerulus and upregulated during glomerulonephritis. Methods: Acute anti-Thy1.1 glomerulonephritis was induced with a single intravenous injection (1 mg/kg body weight) of a monoclonal anti-Thy1.1 antibody in rats. Real-time RT-PCR and immunohistochemical analyses for LTBP-1 and fi brillin-1 were performed. Results: Induction of glomerular LTBP-1 mRNA was detected on day 2 of disease, while mRNA for fi brillin-1 was already upregulated 1 day after induction of disease. Both LTBP- 1 and fi brillin-1 showed a mesangial distribution. An expansion of the LTBP-1 and fi brillin-1-positive mesangial area was seen on day 6 of disease, when transient matrix accumulation was most prominent. On day 12 of disease, glomerular LTBP-1 and fi brillin-1 immunoreactivities had returned to control levels. In serial sections, some colocalization of LTBP-1 and fi brillin-1 was detected in control as well as in nephritic glomeruli. Conclusion: Mesangial expression of LTBP-1 and fi brillin-1 is induced early in experimental nephritis and LTBP-1 and fi brillin-1 are partially colocalized in the nephritic glomerulus. An interaction of these molecules could stabilize latent TGF-β complexes and thus attenuate the activation of TGF- β during this self-limited glomerular disease.

Journal Title
Nephron Experimental Nephrology 2006; 102: e99-e104. <http://content.karger.com/ProdukteDB/produkte.asp?typ=pdf&doi=89688> © 2006 S. Karger AG, Basel
Citation
Nephron Experimental Nephrology 2006; 102: e99-e104. <http://content.karger.com/ProdukteDB/produkte.asp?typ=pdf&doi=89688> © 2006 S. Karger AG, Basel
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