Alterations of global histone H4K20 methylation during prostate carcinogenesis

  • Background Global histone modifications have been implicated in the progression of various tumour entities. Our study was designed to assess global methylation levels of histone 4 lysine 20 (H4K20me1-3) at different stages of prostate cancer (PCA) carcinogenesis. Methods Global H4K20 methylation levels were evaluated using a tissue microarray in patients with clinically localized PCA (n = 113), non-malignant prostate disease (n = 27), metastatic hormone-naive PCA (mPCA, n = 30) and castration-resistant PCA (CRPC, n = 34). Immunohistochemistry was performed to assess global levels of H4K20 methylation levels. Results Similar proportions of the normal, PCA, and mPCA prostate tissues showed strBackground Global histone modifications have been implicated in the progression of various tumour entities. Our study was designed to assess global methylation levels of histone 4 lysine 20 (H4K20me1-3) at different stages of prostate cancer (PCA) carcinogenesis. Methods Global H4K20 methylation levels were evaluated using a tissue microarray in patients with clinically localized PCA (n = 113), non-malignant prostate disease (n = 27), metastatic hormone-naive PCA (mPCA, n = 30) and castration-resistant PCA (CRPC, n = 34). Immunohistochemistry was performed to assess global levels of H4K20 methylation levels. Results Similar proportions of the normal, PCA, and mPCA prostate tissues showed strong H4K20me3 staining. CRPC tissue analysis showed the weakest immunostaining levels of H4K20me1 and H4K20me2, compared to other prostate tissues. H4K20me2 methylation levels indicated significant differences in examined tissues except for normal prostate versus PCA tissue. H4K20me1 differentiates CRPC from other prostate tissues. H4K20me1 was significantly correlated with lymph node metastases, and H4K20me2 showed a significant correlation with the Gleason score. However, H4K20 methylation levels failed to predict PSA recurrence after radical prostatectomy. Conclusions H4K20 methylation levels constitute valuable markers for the dynamic process of prostate cancer carcinogenesis.show moreshow less

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Author:Turang E. Behbahani, Philip Kahl, Johannes von der Gathen, Lukas C. Heukamp, Claudia Baumann, Ines Gütgemann, Bernhard Walter, Ferdinand Hofstädter, Patrick J. Bastian, Alexander von Ruecker, Stefan C. Müller, Sebastian Rogenhofer, Jörg Ellinger
URN:urn:nbn:de:bvb:29-opus-36405
Title of the journal / compilation:BMC Urology 12.5 (2012): 24.10.2012 <http://www.biomedcentral.com/1471-2490/12/5>
Document Type:Article
Language:English
Date of Publication (online):2012/10/24
Publishing Institution:Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU)
Release Date:2012/10/24
Tag:Epigenetics; H4K20; Histone; Methylation; Prostate cancer
SWD-Keyword:-
Original publication:BMC Urology 12.5 (2012): 24.10.2012 <http://www.biomedcentral.com/1471-2490/12/5>
Institutes:Medizinische Fakultät -ohne weitere Spezifikation-
Dewey Decimal Classification:610 Medizin und Gesundheit
Collections:Open Access Artikel ohne Förderung 2012

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