De novo Generation of an Axially Vascularized Processed Bovine Cancellous-Bone Substitute in the Sheep Arteriovenous-Loop Model

Language
en
Document Type
Article
Issue Date
2013-02-25
Issue Year
2011
Authors
Beier, Justus P.
Hess, Andreas
Löw, Johanna
Heinrich, J.
Boos, Anja Miriam
Arkudas, Andreas
Polykandriotis, Elias
Bleiziffer, Oliver
Horch, Raymund E.
Kneser, Ulrich
Editor
Abstract

Abstract Background/Aims: The aim of this study was to generate an axially vascularized bone substitute. The arteriovenous (AV)- loop approach in a large-animal model was applied in order to induce axial vascularization in a clinically approved processed bovine cancellous bone (PBCB) matrix of significant volume with primary mechanical stability and to assess the course of increasing axial vascularization. Methods: PBCB constructs were implanted into 13 merino sheep together with a microsurgically created AV loop in an isolation chamber. The vascularization process was monitored by sequential magnetic resonance imaging (MRI) scans. Explants were subjected to micro-computed tomography (micro-CT) analysis, histomorphometry and immunohistochemistry for CD31 and CD45. Results: Increasing axial vascularization in PBCB constructs was quantified by histomorphometry and visualized by micro-CT scans. Intravital sequential MRI scans demonstrated a significant progressive increase in perfused volume within the matrices. Immunohistochemistry confirmed endothelial lining of newly formed vessels. Conclusion: This study demonstrates successful axial vascularization of a clinically approved, mechanically stable bone substitute with a significant volume by a microsurgical AV loop in a large-animal model. Thus microsurgical transplantation of a tissue-engineered, axially vascularized and mechanically stable bone substitute with clinically relevant dimensions may become clinically feasible in the future.

Journal Title
European Surgical Research 2011; 46: 148-155. <http://content.karger.com/ProdukteDB/produkte.asp?typ=pdf&doi=324408> © 2011 S. Karger AG, Basel
Citation
European Surgical Research 2011; 46: 148-155. <http://content.karger.com/ProdukteDB/produkte.asp?typ=pdf&doi=324408> © 2011 S. Karger AG, Basel
DOI
Document's Licence
Zugehörige ORCIDs